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Trichoderma viride strain and application of metabolites thereof

A technology of Trichoderma viride and strain, applied in the field of microorganisms and agriculture, can solve the problems of unsatisfactory antagonistic effect and high cost, and achieve the effect of reducing aflatoxin pollution

Inactive Publication Date: 2011-08-31
CROP RES INST GUANGDONG ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Although there are many anti-Aspergillus flavus compounds that have been screened, very few of them can be put into practice. The antagonism effect of the screened microorganisms on Aspergillus flavus is not ideal, and the cost is high. It is urgent to find new ones. Antagonistic bacteria to inhibit the growth of Aspergillus flavus

Method used

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  • Trichoderma viride strain and application of metabolites thereof
  • Trichoderma viride strain and application of metabolites thereof
  • Trichoderma viride strain and application of metabolites thereof

Examples

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Effect test

Embodiment 1

[0022] (1) Isolation and identification of Trichoderma viride strains

[0023] ①Separated soil samples were taken from the soil of peanut planting fields in Shantou City, Guangdong Province. Weigh 10g of the soil sample, put it into a conical flask with glass beads filled with 100ml of sterile water, shake it well for 10min to fully mix the soil and water. Under sterile operating conditions, use a pipette gun to draw 1ml from the triangular flask, add it to another test tube filled with 9ml sterile water, mix well, and so on to make 10ml -2 、10 -3 、10 -4 Soil solutions of different dilutions.

[0024] ②Spread culture and streak separation: take 10 -2 and 10 -3 Two concentrations of soil dilutions are used as the object of coating plate culture, and they are coated on PDA (Potato Dextrose Agar) solid medium (200g of potatoes, 20g of glucose, 15g of agar, pH6.8, distilled water to 1000ml) , each sample was replicated 3 times, and after culturing at 28°C for 3 days, observe...

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Abstract

The invention discloses a trichoderma viride strain and application of metabolites thereof and belongs to the field of microorganisms and agriculture. The trichoderma viride strain has the effective antagonistic action on growth of aspergillus flavus, the name of trichoderma viride strain is the trichoderma viride GZ101 which was collected in China Center for Type Culture Collection on Nov. 4th, 2010, and the collection number is CCTCC NO: M 2010291. When the supernatant obtained by fermenting the trichoderma viride strain in the potato-dextrose agar (PDA) liquid culture medium is sprayed in the soil where peanuts are planted, the aflatoxin pollution before the peanut harvest can be effectively reduced, and the reducing rate reaches 97 percent, so that the metabolites of the trichoderma viride strain can be used for preparing a preparation for reducing the aflatoxin pollution before the peanut harvest.

Description

technical field [0001] The invention particularly relates to the application of a Trichoderma viridans strain and its metabolites, and belongs to the field of microorganisms and agriculture. Background technique [0002] Peanut (Arachis hypogaea L.) is an important oilseed and economic crop in my country. Its planting area and total production rank first in the world, and it has strong international competitiveness. For a long time, peanuts are particularly susceptible to aflatoxin contamination. The contaminated peanuts not only greatly reduce the yield and quality, but also seriously threaten human life and health. Therefore, how to reasonably and effectively prevent peanuts from aflatoxin contamination is an important way to improve peanut export trade. important question. [0003] Aspergillus flavus exists in moldy peanuts. In the later stage of growth, Aspergillus flavus produces secondary metabolites, aflatoxins, which can cause toxic damage to tissues and organs of h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 刘付香梁炫强李玲李少雄周桂元洪彦彬温世杰
Owner CROP RES INST GUANGDONG ACAD OF AGRI SCI
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