Aspergillus flavus PEASH-12 not producing aflatoxin and application of aspergillus flavus

A technology of aflatoxin and aflatoxin, applied in the field of microorganisms, can solve the problems of poor field application effect, low survival rate, and poor adaptability, and achieve significant pollution effects, disease reduction, and rapid growth

Active Publication Date: 2019-08-23
SHANDONG PEANUT RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the problems of poor field adaptability, low survival rate and poor field application effect of the existing toxigenic Aspergillus flavus antagonistic bacteria, the purpose of the present invention is to provide a non-toxin-producing strain that efficiently inhibits toxigenic Aspergillus aflatoxus

Method used

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  • Aspergillus flavus PEASH-12 not producing aflatoxin and application of aspergillus flavus
  • Aspergillus flavus PEASH-12 not producing aflatoxin and application of aspergillus flavus
  • Aspergillus flavus PEASH-12 not producing aflatoxin and application of aspergillus flavus

Examples

Experimental program
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Effect test

Embodiment 1

[0028] 1. Isolation, purification and identification of strains

[0029] 1. Sample collection: Samples were collected at the peanut planting site (collected in the Hongqi branch of the Jiangxi Provincial Fine Breed Breeding Farm in 2018.05), and each sample (100g) took 5 sub-samples (2cm wide) within the range of 10×10m by the diagonal method , 5cm deep soil), mixed as a sample. Put the collected samples into plastic bags, prick some pinholes to facilitate gas exchange, transport them to the laboratory, and store them at 4°C for screening of Aspergillus flavus.

[0030] 2. Isolation and purification of bacterial strains.

[0031] (1) Preparation of soil sample bacterial suspension

[0032] Take 10g soil sample, add 90mL 0.1% peptone sterile water (w / v), shake at room temperature for 30min, and make 10 -1 bacterial suspension; then take 0.5mL 10 -1 Add 4.5mL 0.1% peptone sterile water to the bacterial suspension to prepare 10 -2 Bacteria suspension of dilution; prepare 10 ...

Embodiment 2

[0058] The inhibitory effect of embodiment 2 bacterial strain PEASH-12 to toxin-producing Aspergillus flavus

[0059] 1. Laboratory inhibition test

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Abstract

The invention discloses aspergillus flavus PEASH-12 not producing aflatoxin and application of the aspergillus flavu, and belongs to the technical field of microorganisms. The aspergillus flavus PEASH-12 not producing the aflatoxin is stored in China General Microbiological Culture Collection Center on August 1st, 2018, the preservation number is CGMCC NO:15998, the address is No.3, No.1 Beichen West Road, Chaoyang District, Beijing, and the unit requesting preservation is Shandong Peanut Research Institute. Accordingly, the strain can rapidly grow and reproduce in a field, and the growth, reproduction and toxin production of the aspergillus flavus for producing toxicity can be efficiently inhibited, and the effect of field prevention and control over aflatoxin pollution is remarkable; anaspergillus flavus PEASH-12 spore suspension not producing toxicity can not only reduce the occurrence of peanut diseases, but also increase the utilization rate of soil organic matter.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Aspergillus flavus PEASH-12 which does not produce aflatoxin and its application. Background technique [0002] Aflatoxins are metabolites of Aspergillus flavus and Aspergillus parasiticus. A large number of experimental data show that aflatoxin can induce experimental liver cancer in humans and various animals. It is the strongest chemical carcinogen discovered so far, and its ability to induce liver cancer is 75 times greater than that of dimethylnitrosamine. Aflatoxin is also a highly toxic substance. Its acute toxicity is 68 times that of arsenic and 10 times that of potassium cyanide. It can seriously damage the liver and cause death in a short period of time. [0003] Peanuts are the most susceptible crops to be infected with Aspergillus flavus. Peanuts are likely to be infected with Aspergillus flavus during the whole growth process, especi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G22/40A01G7/06A01G13/00C12R1/67
CPCA01G7/06A01G13/00A01G22/40C12N1/14C12N1/145C12R2001/67
Inventor 孙杰张初署赵红军杨伟强毕洁石程仁于丽娜王明清谢宏峰
Owner SHANDONG PEANUT RES INST
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