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Pseudomonas stutzeri and metabolite thereof as well as application of pseudomonas stutzeri YM6 to prevention and treatment of asperigillus flavus and toxins

A technology of Pseudomonas stutzeri and metabolites, applied in application, microorganism-based methods, bacteria, etc., can solve the problems of less research on the prevention and control of Aspergillus flavus and toxins during storage, and achieve the goal of inhibiting the incidence of Aspergillus flavus and toxins. Synthetic, significant bacteriostatic effect, growth-inhibiting effect

Active Publication Date: 2016-11-16
XINYANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been few studies on the storage period control of Aspergillus flavus and toxins. In order to screen efficient storage period biocontrol strains, we screened antibacterial microbial resources from nature, studied their gas production characteristics and antibacterial effects, and carried out storage period research. Study on Prevention and Control of Aflatoxins

Method used

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  • Pseudomonas stutzeri and metabolite thereof as well as application of pseudomonas stutzeri YM6 to prevention and treatment of asperigillus flavus and toxins
  • Pseudomonas stutzeri and metabolite thereof as well as application of pseudomonas stutzeri YM6 to prevention and treatment of asperigillus flavus and toxins
  • Pseudomonas stutzeri and metabolite thereof as well as application of pseudomonas stutzeri YM6 to prevention and treatment of asperigillus flavus and toxins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Isolation and bacteriological identification of candidate strain YM6

[0039] (1) Isolation of candidate strain YM6

[0040] The present invention collects marine flotsam from the Bohai sea area of ​​Yantai City, Shandong Province, China, and isolates a Pseudomonas stutzeri strain with high antibacterial effect on Aspergillus flavus from it through a microbiological screening method. The candidate strain is coded as YM6.

[0041] The NA purification culture method is used for the isolation of microorganisms. The collected floating substances in the ocean are cut into small pieces with scissors, placed on the surface of the NA medium, and cultured at 28°C for 2-3 days. Single colonies of strains with different shapes are picked and analyzed after purification. For the antibacterial effect of a single colony, the strain with better antibacterial effect was selected for preservation for subsequent research.

[0042] (2) Identification of morphological characterist...

Embodiment 2

[0051] Example 2 The antibacterial effect of Pseudomonas stutzeri YM6 on Aspergillus flavus

[0052] Experiment of Inhibition of Mycelia Growth of Aspergillus flavus by Pseudomonas stutzeri YM6

[0053] Method: Petri dishes (diameter 9 cm) were buckled together. Aspergillus flavus spores were inoculated in 50 mL of PDB culture solution (200.0 g of peeled potatoes, boiled in boiling water for 20 min, the filtrate was collected by gauze filtration, 20.0 g of glucose was added, the volume was adjusted to 1 L with distilled water, and the pH was not adjusted), at 28°C and 200 rpm Cultivate under shaking for 3 days, pick the white Aspergillus flavus hyphae mass, and inoculate it in the center of the petri dish containing PDA. Strain YM6 (100 μL, 10 8 cfu / mL) spread on the surface of the culture dish containing NA medium. Buckle the culture dish inoculated with the Aspergillus flavus mycelium block on the culture dish coated with YM6, and seal it with scotch tape for preservatio...

Embodiment 3

[0062] Example 3 Pseudomonas stutzeri YM6 control effect experiment on peanut, corn flavus and toxin:

[0063] Sample Preparation:

[0064] 1) Weigh 100 g of corn and peanut seeds respectively and store them in 250 mL Erlenmeyer flasks. Weigh 3 Erlenmeyer flasks for each crop, sterilize at 121°C and 1.01MPa for 20 min, and let it cool down at room temperature; 2) All Erlenmeyer flasks were inoculated with 1 mL of freshly collected Aspergillus flavus spores (5×10 5 cfu / mL), shake with both hands for 10 min; 3) After adding sterilized water to the three triangle flasks for peanut seeds, adjust the water activity to 0.785, 0.866 and 0.934, respectively, and adjust the water activity to 0.740 for the three triangle flasks for corn kernels , 0.859 and 0.923 spare.

[0065] Approach:

[0066] The antibacterial test was carried out on two crops, peanut and corn, and each crop was treated with three water activities. The three water activities of peanut kernels were 0.785, 0.866 a...

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Abstract

The invention discloses pseudomonas stutzeri YM6 with the preservation number of CCTCC (China Center for Type Culture Collection) M2016386. A metabolite of the pseudomonas stutzeri comprises dimethyl trisulfide. The invention further discloses application of the pseudomonas stutzeri and the metabolite to inhibition of asperigillus flavus and toxins at a storage period. The pseudomonas stutzeri and the metabolite, disclosed by the invention, can be used for effectively inhibiting aspergillus flavus diseases of peanuts, corns and the like and pollution caused by asperigillus flavus toxins under sealed environments with different water activities.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to a strain of Pseudomonas stutzeri, its metabolites and applications thereof. Background technique [0002] Aspergillus flavus ( Aspergillus flavus ) is the most important plant pathogenic fungus producing aflatoxins (Aflatoxins, AFT). It can infect many important grain and oil crops such as peanut, corn, sorghum, soybean and cottonseed. The most heavily polluted toxigenic fungi during storage. AFT produced by Aspergillus flavus is currently the most toxic and carcinogenic substance among known mycotoxins. Among the common types of AFT, aflatoxin B1 (AFB1) is the most toxic, up to 10 times that of potassium cyanide and 68 times that of arsenic. It is easy to induce liver cancer, kidney cancer and rectal cancer after ingestion. In severe cases, it can cause acute death . [0003] The hazards of Aspergillus flavus and AFT are widespread in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23B9/28A23B9/26C12R1/38
CPCA23B9/26A23B9/28C12N1/205C12R2001/38
Inventor 宫安东廖玉才程琳孔宪巍
Owner XINYANG NORMAL UNIVERSITY
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