Aspergillus flavus PEAS-10 which does not produce aflatoxin and application of Aspergillus flavus PEAS-10

A technology of aflatoxin and aflatoxin, applied in the field of microorganisms, can solve the problems of poor field application effect, poor adaptability, and low survival rate, and achieve the effect of significant pollution, rapid growth, and disease reduction

Active Publication Date: 2019-08-16
SHANDONG PEANUT RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Aiming at the problems of poor field adaptability, low survival rate and poor field application effect of the existing toxigenic Aspergillus flavus an

Method used

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  • Aspergillus flavus PEAS-10 which does not produce aflatoxin and application of Aspergillus flavus PEAS-10
  • Aspergillus flavus PEAS-10 which does not produce aflatoxin and application of Aspergillus flavus PEAS-10
  • Aspergillus flavus PEAS-10 which does not produce aflatoxin and application of Aspergillus flavus PEAS-10

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Isolation, purification and identification of strains

[0029] 1. Sample collection: Samples were collected at the peanut planting site (collected in Tangcunba Village, Tumen Township, Jialing District, Nanchong City, Sichuan Province on May 2018). Sub-samples (2cm wide, 5cm deep soil), mixed as one sample. Put the collected samples into plastic bags, prick some pinholes to facilitate gas exchange, transport them to the laboratory, and store them at 4°C for screening of Aspergillus flavus.

[0030] 2. Isolation and purification of bacterial strains.

[0031] (1) Preparation of soil sample bacterial suspension

[0032] Take 10g soil sample, add 90mL 0.1% peptone sterile water (w / v), shake at room temperature for 30min, and make 10 -1 bacterial suspension; then take 0.5mL 10 -1 Add 4.5mL 0.1% peptone sterile water to the bacterial suspension to prepare 10 -2 Bacteria suspension of dilution; prepare 10 as above -3 diluted bacterial suspension.

[0033] (2) Isolat...

Embodiment 2

[0058] The inhibitory effect of embodiment 2 bacterial strain PEAS-10 to toxin-producing Aspergillus flavus

[0059] 1. Laboratory inhibition test

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Abstract

The invention discloses Aspergillus flavus PEAS-10 which does not produce aflatoxin and an application of the Aspergillus flavus PEAS-10 and belongs to the technical field of microorganisms. The Aspergillus flavus PEAS-10 which does not produce aflatoxin is preserved in the General Microbial Center of the Commision for the Protection and Management of Microbial Species in China on August, 1st, 2018, the preservation number is CGMCC NO.:15997, the address is No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, and the unit requiring preservation is Shandong Peanut Research Institute. TheAspergillus flavus PEAS-10 can grow and reproduce rapidly in the field and can effectively inhibit the growth, propagation and toxin production of the Aspergillus flavus, and has remarkable effect onpreventing and controlling aflatoxin pollution in the field; the use of the Aspergillus flavus PEAS-10 can not only reduce the occurrence of peanut diseases, but also increase the utilization rate ofsoil organic matter.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Aspergillus flavus PEAS-10 which does not produce aflatoxin and its application. Background technique [0002] Aflatoxins are metabolites of Aspergillus flavus and Aspergillus parasiticus. A large number of experimental data show that aflatoxin can induce experimental liver cancer in humans and various animals. It is the strongest chemical carcinogen discovered so far, and its ability to induce liver cancer is 75 times greater than that of dimethylnitrosamine. Aflatoxin is also a highly toxic substance. Its acute toxicity is 68 times that of arsenic and 10 times that of potassium cyanide. It can seriously damage the liver and cause death in a short period of time. [0003] Peanuts are the most susceptible crops to be infected with Aspergillus flavus. Peanuts are likely to be infected with Aspergillus flavus during the whole growth process, especia...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C05F11/08C05G3/00C12R1/67
CPCC12N1/14C05F11/08C05G3/00C12R2001/67C12N1/145A01N63/30
Inventor 张初署龚魁杰孙杰于丽娜毕洁王明清栾云霞
Owner SHANDONG PEANUT RES INST
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