Synchronous detection RT-PCR (Reverse Transcription-Polymerase Chain Reaction) kit for yield of aflatoxin and quantity of Nor-1 genetic transcription and detection method of kit

A technology of RT-PCR and aflatoxin, which is applied in the field of simultaneous detection of RT-PCR kits, can solve problems such as false positives, and achieve reliable results, good repeatability, and accurate identification and evaluation
CN109468367AActive Publication Date: 2019-03-15INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
Publication Date
2019-03-15

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Abstract

The invention belongs to the field of biologics and in particular relates to a synchronous detection RT-PCR (Reverse Transcription-Polymerase Chain Reaction) kit for the yield of aflatoxin and the quantity of Nor-1 genetic transcription and a detection method of the kit. The invention establishes a synchronous RT-PCR detection method for synchronously detecting the yield of aflatoxin and the quantity of Nor-1 genetic transcription, and by using the method, the yield of the aflatoxin and the quantity of Nor-1 genetic transcription are in good linear relationship with results of HPLC (High Performance Liquid Chromatography) quantitative aflatoxin and Nanodrop quantitative Nor-1 genetic transcription, therefore, AFT-Nor-1 obtained by using the synchronous detection RT-PCR method has reliableand accurate ratio results which can be used as identification indexes for judging the toxin yields of aspergillus flavus strains, rapid and accurate identification evaluation on the toxin yields of the aspergillus flavus strains is achieved, and the kit has great significances for early warning and control on aflatoxin pollution.
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Description

technical field

[0001] The invention belongs to the field of biology, and in particular relates to a RT-PCR kit for simultaneous detection of aflatoxin output and Nor-1 gene transcription and a detection method thereof. Background technique

[0002] Aflatoxin is the most toxic type of mycotoxin found so far. Taking aflatoxin B1 as an example, its toxicity is 10 times that of potassium cyanide and 68 times that of arsenic. It is listed as a class I carcinogen by the International Cancer Organization. Aflatoxins can easily contaminate grain and oil crops such as peanuts, corn, and rice, and also contaminate many plant products such as walnuts, pistachios, and Chinese herbal medicines. There have been many cases of human and livestock poisoning incidents caused by aflatoxin at home and abroad. According to the latest report from the International Agency for Research on Cancer (IARC), about 500 million people in developing countries alone are at risk of exposure to aflatoxins. ...

Claims

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