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Method for degrading aflatoxin in corn raw material

A technology of aflatoxin and corn, applied in the direction of biofuel, fermentation, etc., to achieve the effect of high moisture content and long reaction time

Inactive Publication Date: 2016-06-08
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are very few applications of biological methods to degrade aflatoxins during corn fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A corn sample seriously polluted by aflatoxin was fermented to produce ethanol according to the existing production process steps (solid-liquid ratio 1:3; liquefaction at 86°C for 90 minutes; simultaneous saccharification and fermentation time at 30°C for 72 hours), the by-product The contents of aflatoxin B1 and B2 in the product DDGS were 434.21±3.02 μg / kg and 25.71±0.30 μg / kg, respectively; in the step of pulping before liquefaction, aflatoxin accounting for 1% of corn flour was added, and the aflatoxin in DDGS The contents of aspergillus toxin B1 and B2 decreased to 9.39±0.26μg / kg and 19.49±0.52μg / kg respectively; the degradation rates of aflatoxin B1 and B2 were 97.8% and 24.2% respectively.

Embodiment 2

[0024]A corn sample seriously polluted by aflatoxin was fermented to produce ethanol according to the existing production process steps (solid-liquid ratio 1:3; liquefaction at 86°C for 90 minutes; simultaneous saccharification and fermentation time at 30°C for 72 hours), the by-product The contents of aflatoxin B1 and B2 in the product DDGS were 434.21±3.02μg / kg and 25.71±0.30μg / kg respectively; 1% of the mass of corn flour was added to the mash after liquefaction and before simultaneous saccharification and fermentation , the content of aflatoxin B1 and B2 in DDGS decreased to 10.25±0.46μg / kg and 20.04±0.15μg / kg respectively; the degradation rates of aflatoxin B1 and B2 were 97.6% and 22.0% respectively.

Embodiment 3

[0026] A corn sample seriously polluted by aflatoxin was fermented to produce ethanol according to the existing production process steps (solid-liquid ratio 1:3; liquefaction at 86°C for 90 minutes; simultaneous saccharification and fermentation time at 30°C for 72 hours), the by-product The contents of aflatoxin B1 and B2 in the product DDGS were 434.21±3.02 μg / kg and 25.71±0.30 μg / kg, respectively; 1% of the mass of corn flour was added to the mash after simultaneous saccharification and fermentation for 36 hours, and DDGS The contents of aflatoxin B1 and B2 in the medium decreased to 15.70±0.87μg / kg and 20.85±0.02μg / kg respectively; the degradation rates of aflatoxin B1 and B2 were 96.4% and 18.9% respectively.

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Abstract

The invention discloses a method for degrading aflatoxin in corn raw material. The method has the advantages that on the basis of the researches on the aflatoxin distribution and conversion rule in the whole process (from corn flour raw material and fermenting mash to mature mash and vinasse after fermentation) of ethanol production through fermentation, mycotoxin degrading agent is used for efficiently degrading the aflatoxin, the aflatoxin content in the vinasse can be lowered to satisfy the aflatoxin limit requirement (not more than 50 microgram / kg) required by national feed hygienic standard (GB 13078), the degrading process and a corn flour fermenting process are synchronous, an existing ethanol production process needs not to be changed, and the ethanol conversion efficiency is unaffected; the method is mild, safe, efficient, and suitable for degrading the aflatoxin in the ethanol production process which uses corn fermentation and is polluted by the aflatoxin.

Description

technical field [0001] The invention discloses a method for degrading aflatoxin, and in particular relates to a method for degrading aflatoxin by adding mildew in the process of fermenting corn raw materials to produce ethanol. Background technique [0002] Aflatoxin is a class of secondary metabolites mainly produced by Aspergillus flavus and Aspergillus parasiticus fungi, and is the most toxic mycotoxin found so far. It can contaminate food and feed through various ways, directly or indirectly enter the human food chain, threaten human health and life safety, and seriously damage human and animal internal organs (especially the liver). The US Centers for Disease Control and Prevention estimates that 4.5 billion people in developing countries may be chronically exposed to aflatoxins through diet. According to one analysis, chronic exposure to aflatoxins causes 25,200 to 155,000 cases of liver cancer each year, especially in Asia and sub-Saharan Africa. According to a roug...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06
CPCC12P7/06Y02E50/10
Inventor 赵仁勇唐怀建岳清华王新伟田双起
Owner HENAN UNIVERSITY OF TECHNOLOGY
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