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Strain producing aflatoxin B1 degrading enzyme and application thereof

A kind of aflatoxin, degrading enzyme technology, applied in the field of microorganisms, can solve problems such as aflatoxin pollution

Inactive Publication Date: 2015-04-08
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The technical problem to be solved by the present invention is to provide a bacterial strain with strong aflatoxin degrading enzyme ability and its application, the enzyme can effectively solve the problem of aflatoxin pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1: Fermentation culture at the shake flask level

[0052] (1) Strain

[0053] bacterial Sinomonas sp.HSD8, CCTCC NO: M2014109.

[0054] (2) Medium

[0055] Each 1 L of the slant medium contains: beef extract 3g, peptone 10g, NaCl 5g, agar 20g, natural pH;

[0056] Each 1 L of the seed medium contains: beef extract 3g, peptone 10g, NaCl 5g, natural pH;

[0057] The fermentation medium at the level of each 1 L of the shake flask contains: 13g corn steep liquor, 2g glucose, 8.5g NaCl, KH 2 PO 4 2.5g, K 2 HPO 4 ·3H 2 O 1g, MgSO 4 1g, 1g coumarin, pH 4.5-5.0 of the fermentation medium.

[0058] (3) Seed cultivation

[0059] After the slant seeds are activated for 24-36 hours, they are inserted into a 250 mL Erlenmeyer flask filled with 50 mL of seed culture medium and cultivated for 12-14 hours at a rotation speed of 160 rpm to obtain first-level seeds.

[0060] (4) Fermentation culture

[0061] The first-level seeds were put into a 50mL / 250mL shake flask and cultured for 7...

Embodiment 2

[0070] Example 2: Fermentation and cultivation on a 50 L fermentor

[0071] (1) Strain

[0072] bacterial Sinomonas sp.HSD8, CCTCC NO: M2014109.

[0073] (2) Medium

[0074] Each 1 L of the slant medium contains: beef extract 3g, peptone 10g, NaCl 5g, agar 20g, natural pH;

[0075] Each 1 L of the seed medium contains: beef extract 3g, peptone 10g, NaCl 5g, natural pH;

[0076] The fermentation medium at the level of 1 L of the fermentor contains: 13g corn steep liquor, 2g glucose, 8.5g NaCl, KH 2 PO 4 2.5g, K 2 HPO 4 ·3H 2 O 1g, MgSO 4 1g, 1g coumarin, the pH of the fermentation medium is 4.5-5.0;

[0077] (3) Seed cultivation

[0078] After the slant seeds are activated for 24-36 hours, they are connected to a 250 mL Erlenmeyer flask filled with 50 mL seed culture medium and cultivated for 12-14 hours to obtain first-level seeds; then, the first-level seeds will be obtained at 10% (V / V) inoculum. The second-level seeds are connected to the same fermentation medium and cultivated f...

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Abstract

The invention discloses a bacterium Sinomonas sp.HSD8 and application thereof. The preservation number of the strain is CCTCC NO:M2014109. After fermentation culture, the activity of aflatoxin B1 degrading enzyme in the fermentation liquid is improved, the aflatoxin pollution problem in feed and food can be effectively solved, and the animal feed and food safety can be guaranteed.

Description

Technical field [0001] The present invention belongs to the field of microbial technology, and specifically relates to a strain of bacteria with strong aflatoxin-detoxifying enzyme capacity Sinomonas sp.HSD8 and its applications. technical background [0002] Aflatoxin is a compound with strong biological toxicity. It is often produced by Aspergillus flavus and other molds in moldy grains, such as rice, beans, peanuts, etc. It is by far the strongest carcinogen , And its toxicity is much higher than that of cyanide, arsenic and organic pesticides. When the human intake is large, acute poisoning can occur, including acute hepatitis, hemorrhagic necrosis, hepatocyte steatosis and bile duct hyperplasia. When ingested continuously in small amounts, it can cause chronic poisoning, growth disorders, fibrotic lesions, and fibrous tissue proliferation. Aflatoxins are mainly B 1 , B 2 , G 1 With G 2 Wait for 4 kinds, and then use B 1 The most toxic. It is generally stable in neutral ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/00A23L1/015C12R1/01A23L5/20
CPCA23L5/25C12N9/00C12N1/205C12R2001/01
Inventor 蔡俊戴军王常高杜馨林建国周安盛
Owner HUBEI UNIV OF TECH
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