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Bacillus siamensis WF2019 strain for degrading aflatoxin B1 and application of bacillus siamensis WF2019 strain

A WF2019, aflatoxin technology, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve the problem that the microbial strains of aflatoxin are not resistant to high temperature and the degradation effect is not significant, and achieve significant degradation performance and high temperature resistance Performance, efficient degradation effect

Active Publication Date: 2019-12-13
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The technical problem to be solved in the present invention is to overcome the defects and deficiencies of the existing microbial strains for degrading aflatoxin that are not resistant to high temperature and the degradation effect is not significant, and provide a Bacillus siamese WF2019 strain for degrading aflatoxin B1 and its application

Method used

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  • Bacillus siamensis WF2019 strain for degrading aflatoxin B1 and application of bacillus siamensis WF2019 strain
  • Bacillus siamensis WF2019 strain for degrading aflatoxin B1 and application of bacillus siamensis WF2019 strain
  • Bacillus siamensis WF2019 strain for degrading aflatoxin B1 and application of bacillus siamensis WF2019 strain

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Effect test

Embodiment 1

[0058] Example 1 Isolation and Identification of Bacillus siamensis WF2019

[0059] 1. Strain isolation and screening

[0060] First, carry out the primary screening of the strain, dilute 1 g of fermented food with 90 mL of sterile normal saline, and make gradient dilutions, spread the diluted solution on a solid medium with coumarin as the only carbon source, culture at 37 ° C, separate and purify the plate Colonies were cultured in a liquid medium with coumarin as the sole carbon source to obtain suspected strains capable of degrading AFB1.

[0061] Then, carry out the re-screening of the bacterial strain, put the suspected bacterial strain into a 10mL centrifuge tube, add 2mL of nutrient broth, and cultivate it at 37°C and 160r / min for 24h to obtain a fermentation broth; ) at 37° C. and 160 r / min for 72 h, and the nutrient broth without the strain plus AFB1 was used as a blank control; after the cultivation, the change of AFB1 concentration was detected by HPLC. It was fo...

Embodiment 2

[0074] Example 2 Degradation performance experiment of Bacillus siamese WF2019 to AFB1

[0075] 1. Experimental method

[0076] Take Bacillus siamese WF2019 in a 10mL test tube, add 5mL of medium, and culture at 30°C, 200r / min for 20h; take 100-300μL of bacterial liquid into a new 5mL medium, so that the OD 600 =0.02~0.05, add AFB1 so that the final concentration of AFB1 is 2 μg / mL, continue to cultivate at 30 °C and 200 r / min for 24 h, 48 h, 72 h, 96 h, and the blank control is sterile medium plus AFB1, the final concentration is 2 μg / mL mL; After the culture, the change of AFB1 concentration was detected by HPLC.

[0077] 2. Experimental results

[0078] The results of the degradation performance of Bacillus siamese WF2019 on AFB1 are as follows: Figure 4 As shown, it can be seen that when cultured for 96 hours, Bacillus siamese WF2019 has the best degradation performance on AFB1, and the degradation rate is as high as 97.7%.

Embodiment 3

[0079] Example 3 Degradation performance experiment of Bacillus siamese WF2019 to different concentrations of AFB1

[0080] 1. Experimental method

[0081] Take Bacillus siamese WF2019 in a 10mL test tube, add 5mL of medium, and culture at 30°C, 200r / min for 20h; take 100-300μL of bacterial liquid into a new 5mL medium, so that the OD 600 =0.02~0.05; add different concentrations of AFB1, so that the final concentration is 1μg / mL, 2μg / mL, 4μg / mL, 8μg / mL, continue to culture at 30℃, 200r / min for 24h, 48h, 72h, 96h, blank The control was sterile medium plus AFB1, and the final concentrations were 1 μg / mL, 2 μg / mL, 4 μg / mL, and 8 μg / mL; after the culture, the change of AFB1 concentration was detected by HPLC.

[0082] 2. Experimental results

[0083] The degradation performance results of Bacillus siamese WF2019 to different concentrations of AFB1 are as follows: Figure 5 As shown, it can be seen that when cultured for 96 hours, Bacillus siamese WF2019 has the best degradation...

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Abstract

The invention discloses a bacillus siamensis WF2019 strain for degrading aflatoxin B1 and an application of the bacillus siamensis WF2019 strain. The bacillus siamensis WF2019 strain capable of efficiently degrading the aflatoxin B1 is successfully separated and obtained, is preserved in Guangdong Microbial Culture Collection Center on June 18th, 2019, and has preservation number of GDMCC NO:60700. The strain can efficiently degrade the aflatoxin B1, and the degradation rate is as high as 98.9%; the environment adaptability is good, the high-temperature resistance is remarkable, and the degradation performance is still remarkable at the temperature of 60 DEG C; meanwhile, fermentation liquor or intracellular substances of the strain can also degrade the aflatoxin B1 and can be applied to detoxification of agricultural products polluted by the aflatoxin B1; in addition, the strain is pollution-free and nuisanceless in the using process. Therefore, the strain is an ideal agricultural product detoxification microorganism, and has good promotion and application prospects in the detoxification aspect of the aflatoxin B1 in the field of feed processing or food processing.

Description

technical field [0001] The invention belongs to the technical field of aflatoxin detoxification. More specifically, it relates to a Bacillus siamese WF2019 strain for degrading aflatoxin B1 and its application. Background technique [0002] Aflatoxin B1 (AFB1) is mainly a mycotoxin produced by Aspergillus parasiticus and Aspergillus flavus, and is currently the most toxic, teratogenic and carcinogenic mycotoxin. At present, AFB1 pollution widely exists in various agricultural products, such as grain crops, food, feed, etc., causing huge economic losses and seriously threatening the health of humans and livestock. In my country, AFB1 pollution is very serious. From January 2015 to April 2019, there were 646 incidents of food mycotoxins exceeding the standard reported in my country, of which AFB1 exceeded the standard incidents accounted for 71%; Toxins accounted for 29.04%, of which AFB1 exceeded the standard events accounted for 97.53%. It can be seen that it is urgent to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L5/20C12R1/07
CPCA23L5/28C12N1/20C12N1/205C12R2001/07
Inventor 王洁房倩安方祥廖振林钟青萍
Owner SOUTH CHINA AGRI UNIV
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