Deep-sea Bacillus aryabhattai capable of inhibiting aflatoxin
A technology of Bacillus aristolochia, deep-sea strains, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of feed and grain nutrient loss, affecting crop growth, and unsatisfactory effects.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0013] Example 1: Liquid fermentation of deep-sea Bacillus Azeri ZL4 strain
[0014] Fresh liquid seeds of ZL4 strain were inoculated into M medium according to 1% of the inoculum. The medium contains 0.5% sodium acetate, 0.05% peptone, 0.05% yeast extract, 0.05% beef extract, 0.05% glucose, and 0.05% Sucrose, 0.05% soluble starch, 0.005% trisodium citrate, 0.005% malic acid, 0.005% potassium sodium tartrate, 0.1% ammonium nitrate, 0.02% ammonium chloride, cultured at 25-30 degrees, 100-160 revolutions per minute After 4-8 days, the fermentation is finished, and the fermentation broth is centrifuged. The cell-free supernatant obtained is the fermentation broth containing the active substance that effectively inhibits aflatoxin, which can inhibit the production of aflatoxin by 100% and can be used directly Prevention and control of aflatoxin pollution, or processed into dry powder to prevent and control aflatoxin pollution.
Embodiment 2
[0015] Example 2: Fermentation broth of deep sea Bacillus Azeri strain ZL4 inhibits the activity of intestinal flora
[0016] Prepare a nutrient plate containing Escherichia coli or aerobacteria in advance, insert Oxford cups in the center and the sides of the plate, add 150 microliters of the cell-free fermentation supernatant obtained in Example 1 into the Oxford cup, and keep it at a low temperature of 4 degrees. After being placed for 24 hours, the plate was placed in a 37°C constant temperature incubator for 2-3 days to detect whether there was a bacteriostatic zone around the Oxford cup. The results showed that there was no bacteriostatic zone around the Oxford cup, which indicated that the deep sea The fermentation broth of Bacillus strain ZL4 has no inhibitory effect on the intestinal flora and has good biological safety.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com