Deep-sea Bacillus aryabhattai capable of inhibiting aflatoxin

A technology of Bacillus aristolochia, deep-sea strains, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of feed and grain nutrient loss, affecting crop growth, and unsatisfactory effects.

Inactive Publication Date: 2015-11-11
HARBIN INST OF TECH AT WEIHAI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] There have been a small number of units at home and abroad that have carried out research on deep-sea microorganisms in the field of biological control, but they mainly focus on the biological control of non-toxigenic fungi, and very little research has been carried out on biological control of toxin-producing fungi, except for this patent application Except for the unit and the applicant, there is no research report on the prevention and control of toxin-producing fungi by deep-sea microorganisms from other units and authors at home and abroad. The harm of toxin-producing fungi is more serious than that of non-toxin-producing fungi, which not only affects the growth of crops, but also reduces the yield. , more importantly, produce mycotoxins that can seriously endanger human

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: Liquid fermentation of deep-sea Bacillus Azeri ZL4 strain

[0014] Fresh liquid seeds of ZL4 strain were inoculated into M medium according to 1% of the inoculum. The medium contains 0.5% sodium acetate, 0.05% peptone, 0.05% yeast extract, 0.05% beef extract, 0.05% glucose, and 0.05% Sucrose, 0.05% soluble starch, 0.005% trisodium citrate, 0.005% malic acid, 0.005% potassium sodium tartrate, 0.1% ammonium nitrate, 0.02% ammonium chloride, cultured at 25-30 degrees, 100-160 revolutions per minute After 4-8 days, the fermentation is finished, and the fermentation broth is centrifuged. The cell-free supernatant obtained is the fermentation broth containing the active substance that effectively inhibits aflatoxin, which can inhibit the production of aflatoxin by 100% and can be used directly Prevention and control of aflatoxin pollution, or processed into dry powder to prevent and control aflatoxin pollution.

Embodiment 2

[0015] Example 2: Fermentation broth of deep sea Bacillus Azeri strain ZL4 inhibits the activity of intestinal flora

[0016] Prepare a nutrient plate containing Escherichia coli or aerobacteria in advance, insert Oxford cups in the center and the sides of the plate, add 150 microliters of the cell-free fermentation supernatant obtained in Example 1 into the Oxford cup, and keep it at a low temperature of 4 degrees. After being placed for 24 hours, the plate was placed in a 37°C constant temperature incubator for 2-3 days to detect whether there was a bacteriostatic zone around the Oxford cup. The results showed that there was no bacteriostatic zone around the Oxford cup, which indicated that the deep sea The fermentation broth of Bacillus strain ZL4 has no inhibitory effect on the intestinal flora and has good biological safety.

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Abstract

The invention provides a deep-sea bacterium capable of inhibiting synthesis of aflatoxin, in particular to Bacillus aryabhattai ZL4, isolated from light yellow calcareous ooze sediment 2796 meters deep in South Atlantic and preserved in China General Microbiological Culture Collection Center under CGMCC No. 10661. The Bacillus aryabhattai ZL4 is fermented in a screened culture medium, acquired fermented supernate is fully capable of inhibiting synthesis of aflatoxin, no toxic intermediates are generated, and the Bacillus aryabhattai ZL4 is able to inhibit the aflatoxin from the toxin synthetic means but has no inhibitory action on intestinal flora and unicellular eumycetes, saccharomyces cerevisiae. The Bacillus aryabhattai ZL4 and its fermentation products are applicable to effective control of aflatoxin contamination of fodders, grains, field crops and the like.

Description

technical field [0001] The invention relates to the technical field of deep-sea microbial technology and mycotoxin biological control, in particular to a strain of deep-sea Bacillus arborii that inhibits aflatoxin. Background technique [0002] In recent years, microbial resources in extreme environments such as oceans and deep seas and their development and utilization have become the focus of world attention and competition. Due to the extreme diversity of living environments, deep-sea microorganisms have strong self-defense capabilities and environmental adaptability, and have extremely important theoretical and It has wide application prospects in agriculture, medicine, food, industry, environmental protection, national defense and other fields. [0003] There have been a small number of units at home and abroad that have carried out research on deep-sea microorganisms in the field of biological control, but they mainly focus on the biological control of non-toxigenic fu...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/07
Inventor 闫培生孙晓磊王凯邵宗泽苏聪史翠娟
Owner HARBIN INST OF TECH AT WEIHAI
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