Hlavobacterium breve biocontrol strain for effectively degrading aflatoxin and application of flavobacterium breve biocontrol strain

A technology of aflatoxin and Flavobacterium brevis, applied in the field of microorganisms

Inactive Publication Date: 2018-05-04
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing research, there is no report on the use of Flavobacterium brevis to degrade aflatoxin

Method used

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  • Hlavobacterium breve biocontrol strain for effectively degrading aflatoxin and application of flavobacterium breve biocontrol strain
  • Hlavobacterium breve biocontrol strain for effectively degrading aflatoxin and application of flavobacterium breve biocontrol strain
  • Hlavobacterium breve biocontrol strain for effectively degrading aflatoxin and application of flavobacterium breve biocontrol strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1) Activate Flavobacterium brevis 3J2MO on the LB plate, culture it in the incubator at 28°C for 24 hours, pick out a single colony of the activated Flavobacterium brevis with a pick, and transfer it to a Erlenmeyer flask containing 15 mL of LB liquid medium , Shaking culture at 28°C, 200r·min-1 for 12h. Aspirate 1% of the culture solution and transfer it to a Erlenmeyer flask filled with 15mL of LB liquid medium, shake and culture at 28°C and 200r·min-1 for 12h to obtain the fermentation solution of the antagonistic strain.

[0019] 2) In the fermentation broth of Flavobacterium brevis (final concentration is 1×10 7 CFU / mL) was added to aflatoxin B1 standard solution and cultured in LB medium, 28°C, 200rpm for 5 days, and 3 replicates were set for each treatment.

[0020] 3) Measure the content of aflatoxin B1 in its culture solution (Table 2).

[0021] Table 2 Degradation effect of biocontrol bacteria on aflatoxin

[0022]

[0023] It can be seen from the experi...

Embodiment 2

[0025] Peanut grains were ground into powder from the peanut field in Hubei, weighed 0.5g of peanut powder in a petri dish, and added 500 μL of Aspergillus flavus spore liquid (5×10 5 cells / mL), cultured in a 28°C incubator for 7 days.

[0026] Add 500 μL of CCTCC No.M 20177329 strain fermentation broth to the spore-covered peanut powder, and culture in 28°C medium for 5 days with an equal amount of LB medium as the control, with 3 replicates in the experiment.

[0027] Add 15mL of 70% methanol water, vortex and place on a shaker for 30min. Take 3mL supernatant, add 8mL ultrapure water and vortex centrifuge;

[0028] 4) Take 8 mL of the supernatant and use the immunoaffinity column-HPLC method to determine the content of aflatoxin B1 (Table 3), and there are 3 repetitions in the experiment.

[0029] Table 3 Control effect of biocontrol bacteria on peanuts contaminated by Aspergillus flavus

[0030]

[0031] It can be seen from the experimental results that the degradatio...

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PUM

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Abstract

The invention belongs to the microorganism field and particularly relates to a flavobacterium breve biocontrol strain for effectively degrading aflatoxin and application of the flavobacterium breve biocontrol strain. The flavobacterium breve biocontrol strain 3J2MO is preserved in China Center for Type Culture Collection on June 13th, 2017 with a preservation number of CCTCC No.M2017329. The adopted flavobacterium breve biocontrol strain is capable of remarkably degrading aflatoxin and has an excellent effect on the degradation of peanuts polluted by aflatoxin. The flavobacterium breve biocontrol strain can be used for degrading aflatoxin and treating the food crops polluted by aflatoxin.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a biocontrol strain of Flavobacterium brevis that can efficiently degrade aflatoxin and its application. Background technique [0002] Aspergillus flavus is a pathogenic fungus that can produce a class of strong carcinogenic and highly toxic mycotoxins - aflatoxins, including B, G and M groups, of which B1 is the most common and the most toxic. It can widely pollute peanuts, corn and other food crops, seriously threaten the health of people and livestock, and cause great economic losses. Therefore, it is urgent to strengthen the control of Aspergillus flavus and toxin pollution. [0003] The traditional methods for removing AFB1 mainly include adsorption, extraction, heat treatment, radiation treatment, acid-base treatment, redox treatment, but sometimes it is time-consuming and laborious, the detoxification rate is not high, and it is easy to cause the loss of nutrient...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L5/20C12R1/01
CPCA23L5/28C12N1/20C12R2001/01C12N1/205A01N63/20
Inventor 李培武张奇王同丁小霞
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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