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Aflatoxin pollution risk early warning molecule and application thereof

An aflatoxin and risk early warning technology, applied in the field of analysis and detection, can solve problems such as lack of early warning methods, and achieve the effects of detection analysis, high sensitivity detection analysis, and high classification accuracy

Active Publication Date: 2021-03-19
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide aflatoxin pollution risk early warning molecules and their application in view of the lack of early warning methods before the occurrence of existing aflatoxin pollution

Method used

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  • Aflatoxin pollution risk early warning molecule and application thereof
  • Aflatoxin pollution risk early warning molecule and application thereof
  • Aflatoxin pollution risk early warning molecule and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] In the following examples, during the establishment of the standard curve: Weigh 200 mg of Aspergillus flavus hyphae into a mortar, grind with liquid nitrogen, and then add 5 mL of PBS buffer solution. It was serially diluted to 0.01, 0.05, 0.1, 0.5, 1, 2, 5, 10, 100 μg / mg mycelia liquid to construct a standard curve. Example 1 Pre-warning molecular screening of aflatoxin-producing strains

[0052] By systematically investigating the metabolic diversity attributes of Aspergillus flavus populations and combining machine learning technology to screen early warning molecules that can effectively distinguish high and low virulence-producing strains, the main steps are as follows:

[0053] Selection of representative samples: Prepared according to standard operating procedures, and select strains from the strain library according to the information of the strain library of the Aspergillus flavus population and according to the geographical origin.

[0054] Sample preparatio...

Embodiment 2

[0082] Example 2 Study on early warning of toxin-producing Aspergillus flavus in agricultural products

[0083] There is a neglected problem in the current risk assessment process of actual peanut samples, that is, usually we only detect whether the aflatoxin in the sample exceeds the standard, and we still know little about the potential risk of the sample that does not exceed the standard. We assume that if the peanut sample is infected by Aspergillus flavus, the humidity and other conditions are not suitable for the growth of Aspergillus flavus, and it is temporarily in a dormant state. At this time, the aflatoxin in the peanut sample does not exceed the standard, and even cannot detect aflatoxin. With suitable humidity conditions, such samples will face a high risk of aflatoxin contamination.

[0084] In order to solve the above existing problems, we use the above-mentioned developed toxin-producing Aspergillus flavus early warning molecules, by adding the peanut samples t...

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Abstract

The invention relates to an aflatoxin pollution risk early warning molecule and application thereof. The method comprises the following steps: weighing a quantitative sample, extracting aflatoxin pollution risk early warning molecules to obtain a sample extracting solution, and detecting and analyzing the sample extracting solution to obtain a quantitative result of the aflatoxin pollution risk early warning molecules; taking the content of one or more aflatoxin pollution risk early warning molecules as a variable, modeling by a chemometrics method to obtain a classification prediction model,and performing risk assessment based on the aflatoxin pollution risk of the classification prediction model sample, wherein the aflatoxin toxin-producing strain early warning molecule is one or a combination of more than one of versiconol (VOH), sterigmatocystin B and 5-MST. The aflatoxin pollution risk early warning molecule discovered by the method has originality, and the early warning method established according to the aflatoxin pollution risk early warning molecule can be used for early warning before aflatoxin pollution occurs.

Description

technical field [0001] The invention relates to a risk assessment method for aflatoxin pollution, belonging to the field of analysis and detection. Background technique [0002] Mycotoxins are secondary metabolites produced by filamentous fungi that can contaminate crops such as peanuts, corn, cotton, and nuts throughout the entire industrial chain. They not only have a high incidence worldwide, causing 25% of the world's annual crops to be polluted by mycotoxins, causing huge economic losses, and seriously endangering people's lives and health. For example, they have carcinogenic, immunosuppressive, hepatotoxic, nephrotoxic, and neurotoxic properties. Aflatoxin is mainly produced by Aspergillus flavus, which is considered to be one of the ten most terrifying fungi in the world. This fungus is widely distributed in the world, including China, and is an important cause of liver cancer in China. At present, many countries have set strict aflatoxin limit standards to ensure ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16C20/20G16C20/70G01N30/88
CPCG16C20/20G16C20/70G01N30/88G01N33/02G01N33/24G01N2030/8872G01N30/06G01N30/72G01N2030/027G01N2030/062
Inventor 李培武张奇谢华里王秀嫔岳小凤白艺珍
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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