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Aspergillus niger and application thereof in biological prevention and control of aflatoxin

An aflatoxin and biological prevention and control technology, applied in the biological field, can solve the problems of high cost and long reaction time of feed factories, and achieve the effects of no pollution to feed and environment, simple operation and high efficiency.

Active Publication Date: 2015-06-03
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the problem is that the reaction time is too long, which causes too much cost for the feed mill
So far, there is no research on the compatibility of Aspergillus niger and tea extracts to synergistically prevent and control aflatoxin

Method used

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  • Aspergillus niger and application thereof in biological prevention and control of aflatoxin

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Experimental program
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Effect test

Embodiment 1

[0037]Aspergillus niger (Aspergillus niger) RAF106 strain, the preservation date is August 27, 2014, the preservation number is CGMCC NO.9608, and the preservation unit is the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microorganisms; the preservation unit address is Beijing, China No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing (100101).

[0038] The formula of the seed medium of Aspergillus niger RAF106 strain is: 200g of potatoes, 20g of sucrose, 20g of agar, 1000mL of distilled water, natural pH, and sterilized at 121°C for 15min. Commercially available potato sucrose agar (PDA) medium can also be used.

[0039] The seed culture conditions of Aspergillus niger RAF106 strain were as follows: eggplant bottles were cultured statically, the culture temperature was 28°C, and the culture time was 4 days.

[0040] Add 0.5% tea extract to the PDA medium and press the addition medium to make a PAD plate, spot RAF106 in th...

Embodiment 2

[0043] Aspergillus niger (Aspergillus niger) RAF106 strain, the preservation date is August 27, 2014, the preservation number is CGMCC NO.9608, and the preservation unit is the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microorganisms; the preservation unit address is Beijing, China No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing (100101).

[0044] The formulation of the seed culture medium of Aspergillus niger RAF106 strain was: 200g potato, 20g sucrose, 20g agar, 1000mL distilled water, natural pH, sterilized at 121°C for 20min. Commercially available potato sucrose agar (PDA) medium can also be used.

[0045] The seed culture conditions of Aspergillus niger RAF106 strain were: static culture in eggplant bottles, culture temperature 30°C, culture time 3 days.

[0046] The method of obtaining conidia from the seeds of Aspergillus niger RAF106 strain was as follows: rinse the eggplant bottle full of conidia with ster...

Embodiment 4

[0050] Aspergillus niger (Aspergillus niger) RAF106 strain, the preservation date is August 27, 2014, the preservation number is CGMCC NO.9608, and the preservation unit is the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microorganisms; the preservation unit address is Beijing, China No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing (100101).

[0051] The formula of seed medium for Aspergillus niger RAF106 and Aspergillus flavus strains was: 200g potato, 20g sucrose, 20g agar, 1000mL distilled water, natural pH, sterilized at 121°C for 20min.

[0052] The seed culture conditions of Aspergillus niger RAF106 and Aspergillus flavus strains were as follows: eggplant bottles were cultured statically, the culture temperature was 30°C, and the culture time was 3 days.

[0053] The method for obtaining conidia from the seeds of Aspergillus niger RAF106 and Aspergillus flavus strains is as follows: rinse the eggplant bottle full ...

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Abstract

The invention discloses Aspergillus niger and application thereof in biological prevention and control of aflatoxin. The strain is Aspergillus niger RAF106 collected in China General Microbiological Culture Collection Center (CGMCC) on August 27, 2014 with a collection number of CGMCC NO.9608 at Yard NO.1, Building NO.3, Beichen West Road, Chaoyang District, Beijing. Under the synergistic effect of plant polyphenol, the competition inhibiting effect of the Aspergillus niger strain screened in the invention is increased greatly. The strain is capable of remarkably inhibiting growth of Aspergillus flavus and generation of aflatoxin, and is also capable of efficiently degrading aflatoxin with high specificity, thereby having very high industrial application values. For agricultural products or feed polluted by aflatoxin, aflatoxin can be decomposed through fermentation of the strain, and the content of aflatoxin is reduced by 80.7% after corn containing aflatoxin is fermented for 48h.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an aspergillus niger and its application in biological control of aflatoxins. Background technique [0002] Aflatoxin is a class of secondary metabolites produced by fungi such as Aspergillus flavus, Aspergillus parasiticus and Aspergillus nomius. Studies have shown that aflatoxins can cause toxic hepatitis, liver cell necrosis, immunosuppression and liver cancer, among which aflatoxin B 1 It has the strongest toxicity and carcinogenicity, and has been designated as a class I carcinogen by the International Agency for Research on Cancer. Food and other crops are highly susceptible to aflatoxin contamination during planting, harvesting, storage and processing. Therefore, aflatoxins have serious negative effects on human and animal health and economic development. [0003] Traditional aflatoxin detoxification methods mainly include physical detoxification and chemical tre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00A23L3/3463A23F3/10A23K1/16C12R1/685
CPCA23B9/26A23B9/28A23F3/10A23L3/3544A23L3/3571A23V2002/00A01N63/30C12N1/145C12R2001/685A23V2250/2132
Inventor 方祥李浩廖振林
Owner SOUTH CHINA AGRI UNIV
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