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Seed bed for culturing ectotrophic mycorrhiza type edible fungus and herbal fungus root

An ectomycorrhizal and edible fungus technology is applied in the fields of application, symbiosis/parasitic combination, botanical equipment and methods, etc. It can solve the problems of time-consuming and laborious transplanting of large trees, and it is difficult to sterilize competing bacteria, and achieves convenient preparation. , low cost, large scale effect

Inactive Publication Date: 2004-11-03
HUNAN ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that since adult trees are often infected by a variety of mycorrhizal fungi, it is difficult to thoroughly disinfect the competing bacteria before infecting the target fungus
Moreover, transplanting large trees is also time-consuming and laborious.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] With the pure culture mycelium of the pine milk mushroom that obtains, inoculate on the solid culture medium that the artificial substrate of high temperature sterilization 35%, perlite 35%, peat soil 28% and nutrient solution 2% is made, in 10 Under the condition of -28 ℃, cultivated for 35 days, inoculated the above-mentioned bacteria-carrying substrate on the seedbed substrate (vermiculite 35%, perlite 35%, peat soil 28% and compound fertilizer 2%) upper layer of non-competitive mycorrhizal miscellaneous bacteria, and well mixed. Then transplant the masson pine seedlings of non-competitive mycorrhizal bacteria that have been cultivated for 25 days in advance to the fungus bed, and cut the roots after 120 days of cultivation under natural conditions. For pine mycorrhizal seedlings, the yield of mycorrhizal seedlings per square meter reaches 80-100 plants. Transplant 11,000 of the above-mentioned qualified mycorrhizal seedlings to 4.5hm 2 On the red loam soil, after ...

Embodiment 2

[0032] Use the isolated lactobacillus strains, under the condition of a solid medium prepared by 30% vermiculite, 40% perlite, 28% peat soil and 2% nutrient solution, culture at controlled temperature and humidity, and then inoculate in aseptic The upper layer of the seedbed substrate (vermiculite 30%, perlite 40%, peat soil 28%) of rhizobacteria, and add 2% of compound fertilizer, mix well, and transplant the masson pine seedlings of aseptic rhizomes that have been cultivated for 25 days in advance Planted on the fungus bed, cultivated under natural conditions for 180 days, then transplanted to 1 hectare of sandy loam soil with purple sandstone without mycorrhizal bacteria at a row spacing of 2×1.5 meters, and obtained the first batch of red juice after 33 months. Milk mushroom fruiting body, obtain the second batch of red milk milk mushroom fruiting body after 39 months, obtain the third batch of fruiting body after 44 months. All the fruiting bodies are completely consisten...

Embodiment 3

[0034] With the isolated milk boletus strain, under the condition of solid medium mixed with 40% vermiculite, 30% perlite, 28% peat soil and 2% nutrient solution, after culturing for 40 days at 23°C, inoculate in Add 2% of compound fertilizer to the upper layer of the seedbed matrix (40% of vermiculite, 30% of perlite and 28% of humus) of competitive mycorrhizal fungi, and mix evenly. The aseptic masson pine seedlings that have been cultivated for 20 days are transplanted to the seedbed, and after being cultivated for 200 days under natural conditions, they are transplanted on the purple sandy soil. After 45 months, the fruiting bodies of milk boletus are obtained, and the number of fruiting bodies per square meter is 10, fresh fruiting body weight 0.52 kg.

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PUM

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Abstract

The present invention is seed bed for culturing mycorrhizic seedling and its obtaining method and culture method. The seed bed for culture exogenous mycorrhizic edible fungi and medicinal fungi has medium of soil and / or artificial medium without competitive foreign fungi. The seed bed has competitive foreign fungi eliminated via sterilization. The present invention provides condition for large scale culture of mycorrhizic fungi in low cost.

Description

technical field [0001] The invention relates to a seedbed for cultivating mycorrhizal seedlings and a method for obtaining the seedbed. The invention also relates to a method for cultivating mycorrhizal shoots. Background technique [0002] Mycorrhizal edible and medicinal fungi refer to a type of fungi that can form a symbiotic relationship with the roots of Pinaceae, Fagaceae, Corylaceae, Benzoinaceae, Betulaceae, Salicaceae and other plants, and have ectomycorrhizal characteristics. These fungi include Tuber melanosporum Vitt., Tuber magnatum Pora., Tuber sinense K. Tao et Liu, Tuber indicum Cooke et Massee, Summer truffle ( Tuber aestivum Vittad.) and other Truffles (Tuberales), Cantharellus cibarius Fr., Thelephora ganbajun Zang, Clavulinopsis miyabeana (Ito.) Ito., etc. Aphyllophorales, Tricholoma matsutake (Ito et Imai)sing.), Boletus edulis bull: Fr., Boletus speciosus Frost, Suillus luteus (L: Fr.) Gray), Suillus greviller (KL.) sing.), Lactarius deliciosus (Fr.)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04A01G7/00A01H17/00
Inventor 谭著明傅绍春陈宏喜张灿明蒋丽娟
Owner HUNAN ACAD OF FORESTRY
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