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Application of mycorrhizal fungi in dendrobe cultivation

A technology of mycorrhizal fungi and dendrobium is applied in the application field of mycorrhizal fungi in the cultivation of dendrobium, and can solve the problems of low content of active ingredients, long cycle, low reproduction and survival rate of dendrobium, etc.

Inactive Publication Date: 2003-08-06
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a kind of application technology of mycorrhizal fungi in the cultivation of Dendrobium, which can solve the problems of low reproduction and survival rate of Dendrobium, slow and long cultivation period, low content of active ingredients and poor drug efficacy.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0025] 1. In this example, Mycena osmundicola is used to activate once from the slant test tube strains preserved at low temperature, and then transferred to PDA plates respectively. When cultivating at a constant temperature of 25°C for 12 days, use a φ8mm hole puncher to place the bacteria on the plates respectively. The edges of the colonies in the medium were punched into bacterial sheets, and the following medium were inserted into the following medium in small pieces.

[0026] 2. Culture of mycorrhizal fungi

[0027] Liquid culture of mycorrhizal fungi: preparation of medium: glucose 2.5%; KH 2 PO 4 0.15%;K 2 HPO 4 0.1%; MgSO 4 0.15%; natural products: wheat bran 3% (cooking juice). After autoclaving, the above-mentioned fungi were inoculated.

[0028] Culture conditions: medium pH 5.6; Erlenmeyer flask shaker culture bottle volume 50%; rotation speed 120 rpm; temperature: 23°C; harvest after 30 days of culture. The inoculum size of the fermenter was 10%, and t...

Embodiment 2

[0042] The basic operating conditions of this embodiment are the same as embodiment one, the difference is:

[0043] 1. The mycorrhizal fungi used in the present embodiment are small mushrooms of Dendrobium dendrobii, small mushrooms of Osmanthus, small mushrooms of orchids and small mushrooms of the lip.

[0044] 2, adopt solid culture medium, the preparation of this culture medium: broad-leaved tree sawdust: wheat bran: corn grain=1: 1: 0.5, the amount of water added is 150-250% (weight ratio) of raw material. After autoclaving, the above-mentioned fungi were inoculated respectively. When mycelium grows from the top of the culture medium to the bottom (about 2 months), take it out for later use.

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Abstract

The present invention relates to an application technique of the mycorrhizal fungi in cultivatino of dendrobium. It is characterized by that the used mycena-generic four fungi respectively are mycenadendrobii, mycena osmundicola, mycena orchidicola and mycena anoectochila. Firstly, the mycorrhizal fungi and dendrobium seedling are respectively cultured, then they are symbiotically cultivated. The adoption of said invented technique can greatly shorten the growth period of dendrobium, and can raise yield of dendrobium and its effective component content.

Description

technical field [0001] The present invention relates to a kind of dendrobium mycorrhizal cultivation technology, specifically relate to a kind of application technology of mycorrhizal fungus in dendrobium cultivation, it comprises the mycorrhizal fungus used in the present invention, the cultivation of mycorrhizal fungus, the development of dendrobium from protocorm to Seedlings and their subculture, the application technology of mycorrhizal fungi in the cultivation of Dendrobium, the growth-promoting effect on Dendrobium and the effect on the content of active ingredients. Background technique [0002] Dendrobium candidum (Dendrobium candidum) and Dendrobium nobile (D. nobile) are recorded in the Pharmacopoeia of the People's Republic of China, and the current commodity is commonly called as Dendrobium. Dendrobium is a traditional precious medicinal plant of Orchidacea in my country. It has the functions of nourishing stomach and promoting body fluid, nourishing yin and cle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04A01G7/00A01H4/00A01H17/00
Inventor 郭顺星陈晓梅宋经元肖培根
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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