Orchid germchit propagating method

A technology for seedlings and spring orchid, which is applied in horticultural methods, botanical equipment and methods, fertilizer mixtures, etc., can solve problems such as the inability of spring orchid to meet the protection of orchid plant resources, shorten the breeding cycle, improve the breeding speed, and overcome slow growth. Effect

Inactive Publication Date: 2010-12-29
SOUTHWEST FORESTRY UNIVERSITY
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Problems solved by technology

[0005] What the present invention aims to solve is the problem that only relying on ramets and tissu

Method used

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Examples

Experimental program
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Example Embodiment

[0016] Example 1: The Chunlan seeds harvested from excellent individual plants in Baoshan, Yunnan Province in 2003 were selected, and seedlings were refined in the greenhouse of the Southwest Forestry College in June 2004. The survival rate of Chunlan seedlings reached 95 % Above, the specific seedling propagation process is as follows:

[0017] (1) Seed germination: The capsules matured in the wild for 180 days are collected, planted in a refrigerator at 4 degrees and refrigerated for 2 weeks, washed with running water for 20 minutes, and the unsplit capsules of Chunlan are taken as explants. Sterilize the surface in 70-75% alcohol for 30-50 seconds, rinse once with sterile water, disinfect with 2% sodium hypochlorite for 20 minutes, rinse with sterile water 4 to 5 times, and then disinfect with 0.1% mercury solution 10-15 minutes, finally rinse with sterile water 5 times. Put the washed mature capsules on sterile filter paper to absorb the water, put the sterilized capsules in...

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Abstract

The invention relates to an orchid germchit propagating method, belonging to an orchid seedling propagating technology, in particular to a propagating technology for seedlings cultured based on tissue culture and mycorrhiza fungi symbiosis. In the orchid germchit propagating method, the germchit can be obtained in the steps of seed germination, successive multiplication of protocorm and rooting culture. The method is characterized in that the rooted seedling can be further acclimatized and the mycorrhiza fungi are mycorrhized after the germchit is obtained by using the propagating method. By using the mycorrhized culture technology based on sterile germination and mycorrhiza fungi symbiosis of the orchid seed, fine varieties can be propagated, fine orchid plants can be selected from the seedlings of seeds, and the propagating speed of the orchid can be quickened, thereby overcoming the defects of slow growth, long cycle and even nonflowering of the pure tissue culture seedling, and having important utility values in the large-area industrialized propagation of the orchid.

Description

technical field [0001] The invention belongs to a technique for growing orchid seedlings, in particular to a technique for growing seedlings through symbiosis of tissue culture and mycorrhizal fungi. Background technique [0002] Orchid refers to the general term of the whole Orchidaceae. In my country, the traditional concept of "orchid" only refers to the Orchidaceae and Cymbidium plants produced in my country. Orchidaceae is a class of precious plants with high ornamental value and economic value. Among them, there are 100 genera and more than 530 species of orchids in Yunnan, ranking first in the country. [0003] But so far, the development and utilization of orchids in my country are still in the primary stage, and the problem of mass reproduction has not been solved, so the contradiction between supply and demand is prominent. On the one hand, driven by economic interests, farmers went to the mountains to dig a large number of wild orchids, which severely damaged w...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01H4/00A01H17/00C05G3/00
Inventor 伍建榕马焕成丁晖胡隽王锦张东华洪英娣刘丽
Owner SOUTHWEST FORESTRY UNIVERSITY
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