Detection method for insertion/deletion marker of goat gene IGF2BP1

Abstract

The invention discloses a detection method for an insertion/deletion marker of a goat gene IGF2BP1. The detection method comprises the following steps: with to-be-detected goat whole-genome DNA as a template, applying a part of fragments of the goat gene IGF2BP1 by PCR, then carrying out agarose gel electrophoresis, and identifying the genotypes of the 15-bp insertion/deletion polymorphic site andthe 5-bp insertion/deletion polymorphic site of the goat gene IGF2BP1 according to electrophoresis results. According to the invention, different genotypes and a combined genotype of 15-bp and 5-bp insertion/deletion polymorphic sites of the goat gene IGF2BP1 have significant correlational relationship with the litter size character of Shaanbei white-cashmere goat, so the 15-bp and 5-bp insertion/deletion polymorphic sites can be used as DNA markers to improve the litter size character of the goat. The detection method for the insertion/deletion marker of the goat gene IGF2BP1 provided by theinvention can be applied to goat molecular marker-assisted selective breeding, and accelerates establishment of an excellent goat genetic resource population.

Description

technical field [0001] The invention belongs to the field of biotechnology and livestock breeding, and relates to a 15-bp insertion / deletion polymorphism (InDel) site of goat IGF2BP1 gene NC_rs647937245:g. Rapid and accurate typing detection of deletion polymorphism (InDel) loci and its application in marker-assisted selection (MAS) breeding. Background technique [0002] The development of new technologies in animal breeding has led to greater changes in production than in the past, but the frequent changes in the growth environment of livestock and poultry, coupled with changes in consumer preferences and palatability, and global population growth, have made animal breeding face the challenge of improving new varieties. Traits related to yield stability and persistence will be the focus of animal breeding. In terms of breeding methods, in addition to making full use of traditional breeding techniques to improve animal traits, in recent years, the use of DNA marker technol...

AI Technical Summary

Problems solved by technology

With the in-depth study of comparative genomics, indel provides a large amount of biological information for theoretical research and genetic breeding application research. As a new generation of genetic identification markers, it mostly focuses on humans and various crops (such as rice and corn, etc.) In the genome research of ruminants, there is little research and application in ruminants

However, there is no report on the significant correlation between the InDel locus of IGF2BP1 gene and litter size and other traits.

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