Method for cultivating water-saving and drought-resistant rice anther

A culture method and culture medium formulation technology, which is applied in the fields of anther culture and plant tissue culture of water-saving and drought-resistant rice, can solve the problems of low culture capacity and difficulty in obtaining pollen plants from callus, and achieve the effect of accelerated breeding

Inactive Publication Date: 2013-12-04
SHANGHAI AGROBIOLOGICAL GENE CENT
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Problems solved by technology

Mainly because indica rice flower cultivation is affected by genotype, the cultivability is low, the average cultivability is on

Method used

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  • Method for cultivating water-saving and drought-resistant rice anther
  • Method for cultivating water-saving and drought-resistant rice anther
  • Method for cultivating water-saving and drought-resistant rice anther

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[0034] Example 1

[0035] figure 2 The effects of different low temperature pretreatment time on the callus induction rate of water-saving and drought-resistant rice were shown.

[0036] Low-temperature pretreatment at the single-nucleate borderline stage is a conventional method to effectively improve the efficiency of flower cultivation. The study believes that the mechanism of low-temperature treatment is to delay the degradation of pollen, maintain the physiological environment for pollen development, increase the level of endogenous auxin and reduce the level of ethylene to initiate androgenesis. et al (Xiao Guoying. Research on anther culture of rice. Hybrid Rice, 1992, (2): 44-46.) In this experiment, three water-saving and drought-resistant rice combinations such as HP1, X17 and R1084 were studied in 0 days, 3 days, Effects of anther callus emergence rate and green shoot differentiation rate under different low temperature pretreatment time of 7 days and 15 days. Th...

Example Embodiment

[0037] Example 2

[0038] image 3 The effect of different heat shock time on the callus induction rate of water-saving and drought-resistant rice was shown.

[0039] The main effect of high temperature heat shock is to accelerate the initiation of microspore division and increase the rate of callus induction. In this experiment, different heat shock times of 0h, 24h, 36h and 48h were used. The results of the three water-saving and drought-resistant rice S624, X41 and S1125 showed that the callus induction rate and the green seedling differentiation rate reached the highest when heat shock at 30℃ for 24h.

Example Embodiment

[0040] Example 3

[0041] Screening and optimization of anther callus induction medium for water-saving upland rice.

[0042] The commonly used media for callus induction of rice anthers include N6, He5, SK3, M8 and improved M8. We used M8 medium, which has good adaptability to indica and japonica rice, as the basic medium, and compared it with other commonly used induction medium. Anther callus induction medium M84 for water-saving and drought-resistant rice, the specific formula is M8 basic medium + 2,4-D 2.0mg·L -1 + NAA 3.0mg·L -1 + KT 1 mg·L -1 , with additional hydrolyzed casein 300 mg L -1 , Proline 300 mg L -1 , Glutamine 500 mg L -1 , Maltose 55g L -1 , phytagel 3.8g L -1 , with a pH of 6.0. Using M84 anther induction medium, the callus usually begins to appear in 20 days, and about 30 days, the anther callus has formed in large quantities. The average recovery rate of japonica water-saving and drought-resistant rice reached 30-50%, and the highest was 66....

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Abstract

The invention relates to a plant tissue culture method, in particular to a method for cultivating water-saving and drought-resistant rice anther, and belongs to the field of biotechnology. According to the adopted technical scheme, the method comprises the following steps: selection of an explant, disinfection and inoculation of the explant, induction and proliferation of a callus, differentiation of the callus, and rooting, cultivating and transplanting of a tissue culture seedling. The method is applied to genetics breeding of water-saving and drought-resistant rice, improves anther culture efficiency of the water-saving and drought-resistant rice, especially indica-type water-saving and drought-resistant rice, shortens breeding cycle, and provides strong technical support for accelerating selection and breeding of fine varieties of the water-saving and drought-resistant rice.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a water-saving and drought-resistant rice anther culture method, which belongs to the field of plant biotechnology seed production and cultivation. Background technique [0002] Rice is one of the most important food crops in my country. In order to further improve the yield potential of rice varieties, in the mid-1990s, my country launched a super-high-yield breeding program to take advantage of heterosis among subspecies, which was later called the Super Rice Program. It has great potential for increasing production, but it is difficult to realize it in large-scale production. The main reason is that more than 65% of the paddy fields in my country are medium-low-yielding fields, and the quality of paddy fields cannot meet the growth needs of existing super rice varieties, and drought is one of the most important limiting factors. Since the 1990s, the average annual drought-aff...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李天菲林田高欢吴金红刘国兰叶水烽黄静艳尹玉周丽
Owner SHANGHAI AGROBIOLOGICAL GENE CENT
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