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Method for cultivating water-saving and drought-resistant rice anther

A culture method and culture medium formulation technology, which is applied in the fields of anther culture and plant tissue culture of water-saving and drought-resistant rice, can solve the problems of low culture capacity and difficulty in obtaining pollen plants from callus, and achieve the effect of accelerated breeding

Inactive Publication Date: 2013-12-04
SHANGHAI AGROBIOLOGICAL GENE CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mainly because indica rice flower cultivation is affected by genotype, the cultivability is low, the average cultivability is only about 0.5%, the highest is no more than 1%, some materials are difficult to induce callus or difficult to obtain pollen plants

Method used

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  • Method for cultivating water-saving and drought-resistant rice anther
  • Method for cultivating water-saving and drought-resistant rice anther
  • Method for cultivating water-saving and drought-resistant rice anther

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] figure 2 The effects of different low temperature pretreatment time on the anther callus induction rate of water-saving and drought-resistant rice were shown.

[0036] Low-temperature pretreatment at the single-nuclei margin stage is a conventional method to effectively improve the efficiency of flower cultivation. Studies have shown that the mechanism of low-temperature treatment is to delay the degradation of pollen and maintain the physiological environment for pollen development. Increase the level of endogenous auxin and reduce the level of ethylene to initiate androgenesis. et al. (Xiao Guoying. Research on Rice Anther Culture. Hybrid Rice, 1992, (2): 44-46.) In this experiment, three water-saving and drought-resistant rice combinations such as HP1, X17, and R1084 were studied in 0 days, 3 days, Effects of 7-day and 15-day low-temperature pretreatment on the anther callus emergence rate and green shoot differentiation rate. The results showed that the healing ra...

Embodiment 2

[0038] image 3 The effects of different heat shock times on the anther callus induction rate of water-saving and drought-resistant rice were shown.

[0039] The main effect of high temperature heat shock is to accelerate the initiation of microspore division and increase the callus induction rate. In this experiment, different heat shock times of 0h, 24h, 36h, and 48h were used. The results of S624, X41, and S1125 three water-saving and drought-resistant rice showed that the callus induction rate and green shoot differentiation rate reached the highest after heat shock treatment at 30°C for 24 hours.

Embodiment 3

[0041] Screening and optimization of anther callus induction medium of water-saving and drought-resistant rice.

[0042] Commonly used media for rice anther callus induction include N6, He5, SK3, M8 and improved M8. We use the M8 medium which has good adaptability to both indica and japonica rice as the basic medium, and compare it with other commonly used induction medium. Anther callus induction medium M84 for water-saving and drought-resistant rice, the specific formula is M8 basic medium + 2,4-D 2.0mg·L -1 + NAA 3.0mg·L -1 + KT 1 mg·L -1 , additional hydrolyzed casein 300 mg·L -1 , proline 300 mg·L -1 , glutamine 500 mg·L -1 , Maltose 55g·L -1 , phytagel 3.8g·L -1 , pH 6.0. Using the M84 anther induction medium, usually 20 days began to callus, 30 days or so, a large number of anther calluses have been formed. The average healing rate of japonica water-saving and drought-resistant rice reaches 30-50%, and the highest can reach 66.2%; the average healing rate of ...

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Abstract

The invention relates to a plant tissue culture method, in particular to a method for cultivating water-saving and drought-resistant rice anther, and belongs to the field of biotechnology. According to the adopted technical scheme, the method comprises the following steps: selection of an explant, disinfection and inoculation of the explant, induction and proliferation of a callus, differentiation of the callus, and rooting, cultivating and transplanting of a tissue culture seedling. The method is applied to genetics breeding of water-saving and drought-resistant rice, improves anther culture efficiency of the water-saving and drought-resistant rice, especially indica-type water-saving and drought-resistant rice, shortens breeding cycle, and provides strong technical support for accelerating selection and breeding of fine varieties of the water-saving and drought-resistant rice.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a water-saving and drought-resistant rice anther culture method, which belongs to the field of plant biotechnology seed production and cultivation. Background technique [0002] Rice is one of the most important food crops in my country. In order to further improve the yield potential of rice varieties, in the mid-1990s, my country launched a super-high-yield breeding program to take advantage of heterosis among subspecies, which was later called the Super Rice Program. It has great potential for increasing production, but it is difficult to realize it in large-scale production. The main reason is that more than 65% of the paddy fields in my country are medium-low-yielding fields, and the quality of paddy fields cannot meet the growth needs of existing super rice varieties, and drought is one of the most important limiting factors. Since the 1990s, the average annual drought-aff...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李天菲林田高欢吴金红刘国兰叶水烽黄静艳尹玉周丽
Owner SHANGHAI AGROBIOLOGICAL GENE CENT
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