Method for fast selecting glutinous rice seed variety
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The technology of a variety, glutinous rice, applied in the field of the rapid breeding method of glutinous rice varieties, can solve the problems of long detection time, low success probability, slow transfer speed, etc., and achieve the effects of fast detection speed, clear breeding goals and low cost.
Inactive Publication Date: 2010-02-03
LIANYUNGANG ACAD OF AGRI SCI
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[0003] 1. Recessive gene backcross transfer method, the classic method in genetics is backcross at generational intervals, but the transfer speed is slow and the breeding cycle is long
[0004] 2. Rice waxy varieties are generally crossed by sticky and waxy varieties, and are bred by pedigree selection. This method requires a large population, slow offspring stability, long breeding cycle, and low probability of success
[0005] 3. Molecular marker selection method: rapid backcrossing can be achieved, but the laboratory conditions are high, and the cost is high and the detection time is long
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Embodiment 1
[0023] Example 1. refer to figure 1 . A kind of quick breeding method for glutinous rice variety, its step is as follows:
[0024] (1) Select an excellent non-waxy variety as the reincarnation parent, select a glutinous rice variety with excellent comprehensive traits, and make the donor parent of the waxy gene wx; 1 , and harvest the reincarnation parent seeds at the same time;
[0025] (2) Plant the seeds of the first generation hybrid and the reincarnation parent, use the reincarnation parent as the female parent, hybridize with the first generation hybrid, and harvest the first generation of backcross BC 1 f 1 with reincarnated parent seeds;
[0026] (3) Planting backcross generation BC 1 f 1 and reincarnation parent seeds; add 0.2 gram of iodine I per 100ml of distilled water 2 Dissolve completely with 2 grams of potassium iodide KI, and make pollen staining solution; select 10 individual plants similar in length to recurrent parents in the first-generation back...
Embodiment 2
[0030] Example 2. refer to figure 1 . A kind of quick breeding method for glutinous rice variety, its step is as follows:
[0031] (1) Select an excellent non-waxy variety such as Xudao 3 as the recurrent parent, choose a waxy rice variety Wandao 68 with excellent comprehensive traits as the donor parent of the waxy gene wx; Xudao 3 as the male parent and The donor parents are crossed, and the hybrid first-generation seeds are harvested F 1 , and harvest the seeds of Xudao No. 3 at the same time;
[0032] (2) Plant the first generation hybrid and Xudao No. 3 seeds, use Xudao No. 3 as the female parent, cross with the first generation hybrid, and harvest the first generation backcross BC 1 f 1 and Xudao No. 3 seed;
[0033] (3) Planting backcross generation BC 1 f 1 With Xudao No. 3 seeds; add 0.2 grams of iodine I per 100ml of distilled water 2Dissolve completely with 2 grams of potassium iodide KI, and make pollen staining solution; select 10 individual plants simi...
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Abstract
A method for fast selecting a glutinous rice seed variety is characterized by selecting a recurrent parent and a donor parent for hybridization, harvesting hybrid seeds F1 of the first generation, andsimultaneously harvesting recurrent parent seeds; repeating the operations till the harvest of six generations of backcross hybrid seeds, and simultaneously harvesting the six generations of backcross hybrid seeds BC6 F1 as well as the recurrent parent; planting the six generations of backcross hybrid seeds BC6 and F1 as well as the recurrent parent; taking down spikes from each plant of the BC6F1 group, carrying out indoor pollen chromoscopy, and eliminating and pulling out single plants with unseparated pollen coloration; when selected plants are single plants extremely similar to the recurrent parent, harvesting the backcross hybrid seeds of the second of the six generations according to each of the single plants; planting various BC6 F2 plants, also planting the recurrent parent forcomparison, selecting seeds from a group extremely similar to the recurrent parent, separately harvesting according to each of the single plants, separately threshing, drying and husking, mixing the seeds of glutinous rice plants so as to form a glutinous rice target variety, and carrying out propagation and typing. The method has high selection speed, increases the possibility of variety breedingsuccess and has low operation cost and a clear target market.
Description
technical field [0001] The invention relates to a method for breeding rice varieties, in particular to a rapid breeding method for glutinous rice varieties. Background technique [0002] The breeding methods of rice varieties in the prior art mainly contain: [0003] 1. Recessive gene backcrossing transfer method, the classic method in genetics is backcrossing at generational intervals, but the transfer speed is slow and the breeding cycle is long. [0004] 2. Waxy varieties of rice are generally crossed by sticky and waxy varieties, and bred by pedigree selection method. This method requires a large population, slow offspring stability, long breeding cycle, and low probability of success. [0005] 3. Molecular marker selection method: rapid backcrossing can be achieved, but the requirements for laboratory conditions are high, and the cost is high and the detection time is long. Contents of the invention [0006] The technical problem to be solved by the present inventio...
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