The invention relates to the technical field of vaccine preparation methods, in particular to a polypeptide vaccine preparation method which comprises the following components in percentage by
mass: 20-30% of a polypeptide
antigen, 10-50% of a carrier, 1-10% of an
adjuvant, 1-3% of a stabilizer and 1-2% of a
preservative. The method comprises the following steps: step 1,
antigen epitope preparation, step 2,
epitope verification, step 3, polypeptide synthesis, step 4, polypeptide loading, step 5, efficiency evaluation, step 6, pretreatment, step 7, purification
processing, step 8,
ultrafiltration processing, step 9, identification, step 10, mixing
processing and the like. A stabilizer and a
preservative are added in the preparation process of the polypeptide vaccine, the stability and safety of the vaccine in the storage and use process can be kept, impurities can be efficiently removed through the steps of physical
precipitation, HPLC purification,
ultrafiltration processing and the like, the quality of the vaccine can be ensured, customized production can be conducted according to different pathogens and epitopes, and the method is suitable for popularization and application. The
medicine is suitable for preventing and treating various diseases.