The invention relates to the technical field of medicines, in particular to a cooling gel sheet and a preparation method thereof. The cooling gel sheet comprises a back lining material, a gel matrix layer and a cover lining material, wherein the gel matrix layer is prepared from menthol, tartaric acid, silanediol salicylate, sodium polyacrylate, glycerin, aluminum glycinate, disodium ethylenediamine tetraacetic acid, a preservative and water. The cooling gel sheet has the advantages that when the cooling gel sheet is stuck, the quick cooling effect is realized by the volatilizing of menthol and gasifying of water; because the silanediol salicylate absorbs a certain amount of menthol, part of menthol slowly volatilizes, the cooling time can be prolonged, and the defects of short action timeand poor suitability in the existing cooling gel sheet are overcome; the preservative contains glycerol octanoate, octyl hydroxamic acid and p-hydroxyacetophenone, so that the moisturizing effect ofglycerol is increased, the function of maintaining the stability of the hydrogel matrix in the gel sheet is realized, the easiness in solidification of the gel matrix due to influence by temperature and overquick evaporation of water is avoided, the stability of the hydrogel matrix in the gel sheet is maintained, and the cooling time is prolonged.