Crossbreeding method in dendrobium test tube

一种杂交育种、试管内的技术，应用在园艺方法、植物学设备和方法、植物基因改良等方向，能够解决育种周期长、难以成功、花粉污染等问题，达到果实发育快、缩短成熟期、开花率高的效果

Active Publication Date: 2013-09-25
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional hybridization of dendrobium orchids takes a long time, and it usually takes 4-6 years to breed a new hybrid; at the same time, it is often difficult to hybridize some species that do not meet in the flowering period. Even if the pollen is preserved at low temperature, it is often polluted by pollen. or lose vitality and fail to succeed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Hybrid breeding of Dendrobium officinale and Dendrobium huoshanense

[0024] 1. Materials: The robust Dendrobium officinale and Dendrobium Huoshanense mother plants were selected for intraspecific artificial cross-pollination during flowering. After 110 days after pollination, the fruits of the two Dendrobium orchids were basically mature, and the seeds in the fruits were selected for aseptic sowing.

[0025] 2. Aseptic sowing: When sowing, first soak the fruit in alcohol with a volume fraction of 75% for 30 seconds, then sterilize it in a mercuric chloride solution with a mass fraction of 0.1% for 20 minutes, rinse with sterile water for 4 times, cut the fruit, and use an inoculation needle The yellow-white powdery embryos are inoculated on the seed germination medium for cultivation. The cultivation conditions are as follows: cultivation temperature 24-28°C, light intensity 1500-2000 lx, light 12 hours / day, seeds begin to germinate in about 12 days, and fo...

Embodiment 2

[0035] Example 2: Hybrid breeding of Dendrobium nobile and Dendrobium unicum

[0036] 1. Materials: When flowering, the vigorously growing mother plants of Dendrobium nobile and Dendrobium unicorn are selected for artificial pollination, and 130 days after pollination, when the fruit is basically mature, the seeds in the fruit are selected for aseptic sowing.

[0037] 2. Aseptic sowing: When sowing, first soak the fruit in alcohol with a volume fraction of 75% for 30 seconds, then sterilize it in a mercuric chloride solution with a mass fraction of 0.1% for 20 minutes, rinse with sterile water for 4 times, cut the fruit, and use an inoculation needle The yellow-white powdery embryos are inoculated on the seed germination medium, and the culture conditions are: culture temperature 24-28°C, light intensity 1500-2000 lx, light 14 hours / day, seeds start to germinate in about 12 days, and form protocorms. Complete plantlets can be formed in 75 days.

[0038] 3. Test-tube floweri...

Embodiment 3

[0047] Example 3: Hybrid Breeding of Dendrobium Red Star and Dendrobium Pink Lady

[0048] 1. Materials: When blooming, select the robust spring Dendrobium hybrids Red Star and Pink Lady for artificial pollination. 150 days after pollination, when the fruit is basically mature, select the seeds in the fruit for aseptic sowing.

[0049] 2. Aseptic sowing: When sowing, soak the fruit in alcohol with a volume fraction of 75% for 30 seconds, then sterilize it in a mercury solution with a mass fraction of 0.1% for 20 minutes, rinse with sterile water for 5 times, cut the fruit, and use an inoculation needle. The yellow-white powdery embryos are inoculated on the seed germination medium, and the culture conditions are: culture temperature 24-28°C, illuminance 1500-2000lx, light 16 hours / day, seeds start to germinate in about 15 days, and form protocorms. Complete plantlets can be formed in 90 days.

[0050] 3. Test-tube flowering induction and cross-breeding in test tube: Transfe...

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PUM

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Abstract

The invention discloses a crossbreeding method in a dendrobium test tube. According to the invention, the crossbreeding method in the dendrobium test tube is adopted, the ripening period of fruits can be shortened greatly, and crossbreeding generations in the test tube can bloom within a short period of time, and flower patterns and colors can be observed, and novel varieties are bred, thereby accelerating the dendrobium breeding speed, and the crossbreeding method is reported for the first time internationally; because dendrobium in the test tube can bloom annually, and can crossbreed with varieties rare in flower seasons in the natural world. Additionally, according to the invention, culture medium in each stage is utilized, 'hyponex' is utilized as the main component, and the cultural media are unique in ingredient, not only are low in cost, but also are high in abloom rate, and the fruits are developed rapidly; moreover, only a simple plant tissue culture device is required to implement the crossbreeding method provided by the invention, and therefore the whole breeding method is simple and convenient, is low in cost, and creates conditions for culturing the high-quality dendrobium variety.

Description

Technical field: [0001] The invention belongs to the field of plant breeding, and in particular relates to a dendrobium in vitro hybrid breeding method. Background technique: [0002] There are about 1,600 species of Dendrobium plants, and there are more than 70 species in my country. Most species have high ornamental value and are one of the most important species in the world's orchid industry. The popular ornamental Dendrobium species on the market are mostly Hybrid breed. Conventional hybridization of dendrobium orchids takes a long time, and it usually takes 4-6 years to breed a new hybrid; at the same time, it is often difficult to hybridize some species that do not meet in the flowering period. Even if the pollen is preserved at low temperature, it is often polluted by pollen. Or lose vitality and be difficult to succeed. Invention content: [0003] The object of the present invention is to provide a kind of hybrid breeding method in test tube of dendrobium orchid ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/005
Inventor 曾宋君吴坤林张建霞郑枫段俊
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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