Method for detecting X-type HMW-GS (High Molecular Weight Glutenin Subunit) gene in distant hybridization wheat crossbreed

A distant hybridization and gene technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the complicated operation and cannot be transferred from foreign sources. Target gene tracking, identification, and evaluation standards It is difficult to accurately grasp and other problems, so as to achieve the effect of reliable results and simple identification methods.
CN103114134BActive Publication Date: 2014-06-25SICHUAN AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SICHUAN AGRI UNIV
Publication Date
2014-06-25

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Abstract

The invention provides a method for detecting an aegilops sharonensis X-type HMW-GS (High Molecular Weight Glutenin Subunit) gene in a distant hybridization wheat crossbreed. The invention further provides a primer for detecting the aegilops sharonensis X-type HMW-GS gene. The nucleotide sequence of the primer is shown in SEQ ID NO. 1-2. Whether the distant hybridization wheat crossbreed contains an aegilops sharonensis X-type HMW-GS genetic locus can be rapidly and accurately detected by carrying out PCR (Polymerase Chain Reaction) through using the primer. By utilizing the method, the transfer and existence of the aegilops sharonensis X-type HMW-GS gene can be accurately detected, the gene can be conveniently tracked, the working efficiency in breeding is greatly improved, and the progress in wheat quality breeding is accelerated, so that the method has good application prospects in the field of crossbreeding of wheat species.
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Description

Technical field

[0001] The present invention relates to the field of molecular genetics, in particular to a primer for detecting Aegilops sylvestris X-type high molecular weight glutenin subunit (HMW-GS) gene. The present invention also relates to the use of the primer for wheat remote Method and kit for detecting X-type gluten subunit gene in hybrids and offspring. Background technique

[0002] The wild relatives of wheat have many excellent gene resources that are lacking in the common wheat gene bank, and they are valuable resource banks that can be utilized. So far, through chromosome engineering technology, people have introduced beneficial genes from related germplasms such as rye, wheatgrass, Elytrigia intermedius, Elymus dahurica, Aegilops tall, and barley. For example, Aegilops species have resistance to stripe rust, leaf rust, stalk rust, powdery mildew, drought tolerance, salt-alkali tolerance, cold tolerance, high protein and other excellent genes that are important ...

Claims

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