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Methods for creating and identifying Cucumis sativus-Cucumis hystrix Chakr disomy additional line

An identification method, the technology of pickled cucumber, applied in the field of plant biotechnology breeding, can solve the problems that the two-body addition system has not been reported, and achieve the effect of low cost and simple and convenient operation process

Inactive Publication Date: 2020-09-29
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, there has not been any report on the selection of disomic addition lines in Melon

Method used

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  • Methods for creating and identifying Cucumis sativus-Cucumis hystrix Chakr disomy additional line
  • Methods for creating and identifying Cucumis sativus-Cucumis hystrix Chakr disomy additional line
  • Methods for creating and identifying Cucumis sativus-Cucumis hystrix Chakr disomy additional line

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Embodiment 1

[0039] The creation and identification method of the material of the cucumber-pickle disomy addition line material carrying the No. 10 chromosome of pickled cucumber comprises the following steps:

[0040] (1) Creation of allotriploid: using the allotetraploid material (C.hytivus) obtained from the interspecific hybridization of cucumber obtained by Chen Jinfeng and the wild species of the same genus - sour cucumber (C. hytivus) (Chen J F, Luo X D, Staub J E , et al. Anallotriploid derived from a amphidiploid×diploid mating in Cucumis-I: Production, micropropagation and verification [J]. Euphytica, 2003, 131 (2): 235-241.) and diploid cultivated cucumber (C.sativus ) backcross to create allotriploid material.

[0041] (2) Creation of the monosomatic addition line of cucumber-pickle cucumber: backcross the allotriploid and diploid cultivars to obtain the candidate isoaddition line material, and the candidate isoaddition line was screened out by genome in situ hybridization. Ad...

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Abstract

The invention discloses methods for creating and identifying a Cucumis sativus-Cucumis hystrix Chakr disomy additional line, and belongs to the field of plant biotechnology breeding. The method comprises the following steps: backcrossing a Cucumis sativus-Cucumis hystrix Chakr interspecific hybridization allotetraploid and a diploid cultivated cucumber twice to obtain a Cucumis sativus-Cucumis hystrix Chakr monomer alien addition line, performing selfing through the Cucumis sativus-Cucumis hystrix Chakr monomer alien addition line, and screening out a Cucumis sativus-Cucumis hystrix Chakr diploid additional line through a molecular marking method and a genome in-situ hybridization method. The method is simple and convenient in operation process and low in cost, the Cucumis sativus-Cucumishystrix Chakr disomy additional line material carrying the chromosome 10 of the sour cucumber is created for the first time, and a foundation is laid for excavating excellent genes of the Cucumis hystrix Chakr and further realizing genetic improvement of cultivated cucumber.

Description

technical field [0001] The invention belongs to the field of plant biotechnology breeding, and in particular relates to a method for creating and identifying a cucumber-pickled cucumber two-body addition line, which can be used for transferring excellent genes of wild pickled cucumbers and genetic improvement of cultivated cucumber varieties. Background technique [0002] Cucumber (Cucumis sativus L., 2n=2x=14) originated in India and is a very important vegetable crop. So far, due to the long-term pursuit of high-yield and directional selection of cucumbers, the variety of cucumbers has become single and the genetic basis has become narrower (Staub et al., 1999). At the same time, cucumber pests and diseases have seriously threatened the production of cucumbers. The spread of diseases such as downy mildew and powdery mildew has caused a large area of ​​cucumber yield reduction (Xiong Yan et al., 2016; Shen Hong et al., 2017). Therefore, it is a safe and efficient way to in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H5/00A01H6/34C12Q1/6895C12Q1/6841
CPCA01H1/02A01H5/00A01H6/346C12Q1/6841C12Q1/6895C12Q2600/13C12Q2563/107
Inventor 娄群峰李梦雪刘昱希陈劲枫
Owner NANJING AGRICULTURAL UNIVERSITY
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