A culture medium for inducing embryogenic callus of inbred line of common wild rice in Southeast Asia

Abstract

The invention provides an induction medium of a selfing line embryonic callus of Southeast Asia common wild rice. The induction medium is obtained by adding specific hormone of specific concentration to the base of a N6D culture medium, and by performing induction culture on the culture medium, a callus rate is improved, a callus diameter is increased and a callus quality is improved.

Description

technical field [0001] The invention relates to an induction medium and an induction method, in particular to an induction medium and an induction method for the embryogenic callus of the inbred line of Oryza sativa in Southeast Asia. Background technique [0002] Plant dedifferentiation and differentiation are affected by many factors, such as genotype, explant selection, medium type and the interaction between them, but so far, it has not been possible to establish a "universal culture" suitable for all genotypes. base” (Bolibok, 2006). However, the genome of wild rice is quite different from that of cultivated rice, so the culture system suitable for cultivated rice cannot produce the same positive effect on all wild rice. For example, the in vitro culture of medicinal wild rice is limited by genotype, and it is difficult to obtain embryogenic callus of medicinal wild rice (Ding Yumei, 2003); the efficiency of high stem wild rice leaves to induce callus is low (Ni Wenjin...

AI Technical Summary

Problems solved by technology

[0002] Plant dedifferentiation and differentiation are affected by many factors, such as genotype, explant selection, medium type and the interaction between them, but so far, it has not been possible to establish a "universal culture" suitable for all genotypes. Base" (Bolibok, 2006)

However, the genome of wild rice is quite different from that of cultivated rice, so the culture system suitable for cultivated rice cannot have the same positive effect on all wild rice

For example, the in vitro culture of medicinal wild rice is limited by genotype, and it is difficult to obtain embryogenic callus of medicinal wild rice (Ding Yumei, 2003); the efficiency of high-stem wild rice leaves to induce callus is low (Ni Wenjin, 2007); however, mature plants can be differentiated from cryopreserved callus of Oryza sativa (Zeliang, 2010)

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