A Molecular Marker and Special Primers Used to Identify the Restorer Line of Cytoplasmic Male Sterile Line of Common Wild Rice in Yuanjiang, Yunnan

Abstract

The invention discloses a molecular marker and special primers used for identifying a restorer line of a cytoplasmic male sterile line of common wild rice in Yuanjiang Yunnan. The nucleotide sequence of one of the special primers used for assisting identification of the restorer line of the cytoplasmic male sterile line of the common wild rice in Yuanjiang Yunnan is shown as the sequence 1 in the sequence table, and the nucleotide sequence of the other special primer is shown as the sequence 2 in the sequence table. The molecular marker and the special primers can be used for breeding the restorer line of the cytoplasmic male sterile line of the common wild rice in Yuanjiang Yunnan, shortening the breeding period of three-line hybrid rice containing high-quality genetic resources of the common wild rice in Yuanjiang Yunnan, accelerating the breeding speed, and reducing the breeding cost. The primers and the molecular marker have advantages of simple operation, low cost, and short period, are suitable for popularization and application, and provide a rapid selection method for the restorer line having the genetic background of the common wild rice in Yuanjiang Yunnan.

Description

technical field [0001] The invention relates to a molecular marker and a special primer for identifying a restorer line of a cytoplasmic male sterile line of common wild rice Yuanjiang, Yunnan. Background technique [0002] Rice is one of the most important food crops in the world, and increasing rice yield per unit area is one of the powerful measures to ensure world food security. In the long-term evolution process of crops, because breeders select target traits according to needs and carry out directional breeding and repeated use of parents, the genetic basis of varieties is narrow. At present, more than 95% of the hybrid rice hybrids that account for more than half of the rice planting area are composed of wild-type or similar cytoplasmic male sterile lines, and long-term use of single-source cytoplasm , there may be a risk of genetic vulnerability. [0003] Wild rice is not only an important physical basis for studying the origin, classification, and evolution of ric...

AI Technical Summary

Problems solved by technology

The male sterile cytoplasm used in the production of hybrid rice in my country is mainly derived from common wild rice. It can be speculated that there must be a corresponding restorer gene in wild rice (Li Shaoqing et al., 2005), but the restorer gene used in production comes from wild rice. but very few

Images