Rapid propagation method of Oryzarufipogon Griff by tissue culture

A tissue culture, wild rice technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of difficulty in harvesting mature seeds, difficulty in reproduction, strong seed dormancy, etc., to improve the quality of seedlings, improve Multiplication coefficient, the effect of improving germination rate

Active Publication Date: 2017-11-10
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For ordinary wild rice, it is difficult to harvest mature seeds, and the seeds are very dormant, which brings certain difficulties to reproduction

Method used

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  • Rapid propagation method of Oryzarufipogon Griff by tissue culture
  • Rapid propagation method of Oryzarufipogon Griff by tissue culture

Examples

Experimental program
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Embodiment 1

[0026] A kind of common wild rice tissue culture rapid propagation method of the present embodiment comprises the following steps:

[0027] (1) Selection and disinfection of explants: take ordinary wild rice seeds as explants, remove the rice husk, soak for 5 minutes with a 1% detergent solution by volume, rinse with tap water for 10 minutes, and place at 75 % ethanol for 1 min, then soaked in 0.003% sodium hypochlorite solution containing 2% active oxygen for 5 min, finally rinsed with sterile water for 3 times, and blotted the surface moisture with sterilized filter paper to obtain explants; Sterile water is distilled water through autoclaving;

[0028](2) Germination of seeds to obtain aseptic test-tube plantlets: inoculate the explants obtained in step (1) into the first medium, and cultivate them in the dark at 22°C for 18 days, and obtain aseptic test-tube plantlets after the seeds germinate; The first medium is prepared by adding GA3 0.1mg / L, sucrose 20mg / L, agar 3mg / L...

Embodiment 2

[0034] A kind of common wild rice tissue culture rapid propagation method of the present embodiment comprises the following steps:

[0035] (1) Selection and disinfection of explants: take ordinary wild rice seeds as explants, remove the rice husk, soak for 15 minutes with a 2% detergent solution by volume, rinse with tap water for 20 minutes, and place them at 75 % ethanol for 2 min, then soaked in 0.006% sodium hypochlorite solution containing 2% active oxygen for 10 min, and finally rinsed with sterile water for 5 times, and blotted the surface moisture with sterile filter paper to obtain explants; Sterile water is distilled water through autoclaving;

[0036] (2) Germination of seeds to obtain aseptic test-tube plantlets: inoculate the explants obtained in step (1) into the first medium, and cultivate them in the dark at 26°C for 24 days, and obtain aseptic test-tube plantlets after the seeds germinate; The first medium is prepared by adding GA3 0.5mg / L, sucrose 25mg / L, a...

Embodiment 3

[0042] A kind of common wild rice tissue culture rapid propagation method of the present embodiment comprises the following steps:

[0043] (1) Selection and disinfection of explants: take ordinary wild rice seeds as explants, remove the husk, soak for 10 minutes with 1.5% detergent solution by volume, rinse with tap water for 15 minutes, and place them at 75 % ethanol for 1.5min, then soaked in 0.005% sodium hypochlorite solution containing 2% active oxygen for 8min, finally rinsed with sterile water for 4 times, and dried the surface moisture with sterile filter paper to obtain explants; , sterile water is distilled water through autoclaving;

[0044] (2) Germination of seeds to obtain aseptic test-tube plantlets: inoculate the explants obtained in step (1) into the first medium, and cultivate them in the dark at 24°C for 20 days, and obtain aseptic test-tube plantlets after the seeds germinate; The first medium is prepared by adding GA3 0.4mg / L, sucrose 23mg / L, agar 4mg / L ...

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Abstract

The invention relates to the technical field of rapid propagation of plants, in particular to a rapid propagation method of Oryzarufipogon Griff by tissue culture. The method comprises the steps as follows: selecting and disinfecting rice seeds; inoculating the seeds into a culture medium containing seaweed extracts to obtain sterile test tube seedlings; inoculating the test tube seedlings into a medium containing aloe extracts to obtain cluster buds; inoculating the cluster buds into a medium containing wheat germ extracts to obtain robust plants; transplanting the robust plants into a medium containing chlorella extracts for culture to obtain complete plants with roots. Compared with a traditional method, the method has the advantages that by optimizing the medium at each stage of rapid propagation, the germination rate of the wild rice seeds is further increased, propagation and differentiation of the cluster buds are promoted, rooting capacity of new plants is enhanced, obtained new seedlings grow well and are robust, and the success rate of rapid propagation of tissue culture of wild rice is significantly increased.

Description

【Technical field】 [0001] The invention relates to the technical field of plant rapid propagation, in particular to a method for rapid propagation of common wild rice tissue culture. 【Background technique】 [0002] Common wild rice (Oryzaru fipogon Griff.) is a grass plant, the ancestor of rice, and a national second-class protected plant. Archaeologists identified the appearance characteristics of the unearthed rice stalks and leaves, which proved that China had cultivated rice 7,000 years ago, more than 1,000 years earlier than Thailand, which is rich in rice. Common wild rice is the close ancestor of rice. It has good compatibility and high hybrid seed setting rate. It provides precious genetic data for rice breeding and provides a theoretical basis for elucidating the origin and evolution of rice. It has important scientific value. [0003] Plant tissue culture began at the beginning of the 20th century. After that, this technology developed rapidly, penetrated into the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 邱永福李志华林杰斌李洋王心怡刘芳李容柏
Owner GUANGXI UNIV
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