Gene for increasing protein content of japonica rice and application

A protein content and protein technology, applied in the field of plant biology technology and genetic engineering, can solve the problems that affect the competitiveness of rice, cannot meet the consumption demand of high-quality rice, and the quality of rice is low

Inactive Publication Date: 2016-11-16
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the quality of rice in my country is generally low. The quality of indica rice cultivated in large areas in the south is generally lower than the second grade of the national standard, and the quality of japonica rice cultivated in the north is also far behind that of Japanese varieties.
On the one hand, this cannot meet the growing consumption demand of Chinese consumers for high-quality rice, and also seriously affects the competitiveness of Chinese rice in the world market

Method used

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  • Gene for increasing protein content of japonica rice and application
  • Gene for increasing protein content of japonica rice and application
  • Gene for increasing protein content of japonica rice and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: QTL mapping of rice protein content

[0045] 1. Phenotype measurement of rice protein content

[0046] After the grains are naturally dried, they are placed at room temperature for at least 3 months to ensure that the grains are dry and the moisture content of each strain is relatively consistent. The brown rice is obtained by peeling the hulls on the rice unpolishing machine, and the brown rice is ground into polished rice on the rice polishing machine. Quickly detect the protein content of polished rice on a near infrared rapid analyzer.

[0047] 2. Identify QTLs for protein content in polished rice using single-fragment substitution lines

[0048] From 2007 to 2008, the protein content of the typical indica rice variety Habataki and the japonica rice variety Sasanishiki were identified in three different environments, and it was found that there were significant differences in protein content in different environments (Table 1). In the 39 chromosome single-segme...

Embodiment 2

[0051] Example 2: Fine localization and map-based cloning of qPC-10

[0052] 1. The development of molecular markers

[0053] The SSR mark information used in the present invention is all from the Gramene website database (http: / / www.gramene.org. / ). In addition, according to the genome sequence of the japonica rice variety Nipponbare ( http: / / rgp.dna.affrc.go.jp ), and the genome sequence of the indica variety 93-11 ( http: / / rise.genomics.org.cn / ) Designed a polymorphic InDel (Insert / Deletion) mark between Habataki and Sasanishiki for the fine positioning analysis of qPC-10.

[0054] 2. Analysis of recombinant individual plants, fine mapping of qPC-10 and determination of candidate genes

[0055] The genotypes and phenotypes of 39 single-segment substitution line populations were identified. SL431 was considered to contain qPC-10 and its polished rice protein content was significantly higher than that of the background parent Sasanishiki. SL431 is based on Sasanishiki and has o...

Embodiment 3

[0056] Example 3: Candidate gene expression and transgene verification of qPC-10

[0057] Real-Time expression profile analysis found that the gene is only expressed in the endosperm and not expressed in other tissues during the whole growth period. In order to further clarify the cause of the protein content variation caused by the qPC-10 gene, we constructed a qPC-10 gene Near isogenic line, NIL-qPC-10 H And NIL-qPC-10 S . The expression of qPC-10 gene was analyzed by real-time RT-PCR. The results show that qPC-10 from the indica rice Habataki H The allele expression is significantly higher than qPC-10 in japonica rice S Allele (attached figure 2 ).

[0058] In order to verify the function of the qPC-10 gene, according to the predicted full-length cDNA sequence of the candidate gene (2001-3500bp of SEQ ID NO. 1), a pair of specific primers were designed to amplify the Habataki promoter and coding region sequence, and then ligated to TA On the cloning vector, the correct clone ...

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PUM

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Abstract

The invention belongs to the field of the plant biological technology and the gene engineering technology, and particularly relates to a gene for increasing the protein content of japonica rice and application, in other words, gene cloning and application of main effective QTL (qPC-10) controlling the protein content of endosperm of rice. The gene of rice qPC-10 has the DNA sequence shown as SEQ ID No.1, and the amino acid sequence of the coded protein of the gene of rice qPC-10 is shown as SEQ ID No.2. The QTLqPC-10 gene can control the protein content of rice and can be stably inherited. By using the gene in japonica rice, the protein content can be increased by 1%, and a novel rice variety with high nutrient value can be cultured.

Description

Technical field [0001] The invention belongs to the field of plant biology technology and genetic engineering technology, and specifically relates to the gene cloning and application of a major QTL (qPC-10) controlling the protein content of rice endosperm. Background technique [0002] More than half of the world's population uses rice as their main food source. In the past ten years, with the continuous improvement of people's living standards, the quality of rice has attracted more and more consumers' attention. However, the quality of rice in my country is generally low. The quality of indica rice cultivated in a large area in the southern rice region is generally lower than the second national standard, while the quality of japonica rice cultivated in the north is far from Japanese varieties. This aspect cannot meet the growing consumer demand of Chinese consumers for high-quality rice, and it also seriously affects the competitiveness of Chinese rice in the world market. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82C12N5/10A01H5/00
CPCC07K14/415C12N15/8243
Inventor 严长杰杨宜豪郭旻
Owner YANGZHOU UNIV
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