Efficient japonica hybrid rice selective-breeding method capable of reducing seed production cost
A low-cost hybrid japonica rice technology, applied in botany equipment and methods, application, plant gene improvement, etc., can solve the problems of high seed cost and low seed production yield, achieve efficient breeding, high mutation frequency, and improve seed production yield effect
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Embodiment 1
[0024] Shenfan 24 is a high-quality and strong dominant hybrid japonica restorer line bred by the Institute of Crop Breeding and Cultivation of Shanghai Academy of Agricultural Sciences. , high-yield, and disease-resistant hybrid japonica rice combination, among which Shen 01A / Shen Fan 24 passed the Shanghai variety approval (named 'Shen You 24', approval number: Shanghai Approved Rice 2015001), but Shen Fan 24 has a long growth period The problem. To solve this problem, in the winter of 2011, Shanghai Shuneng Irradiation Technology Co., Ltd. used 350Gy 60 Coγ-rays irradiated Shenfan 24 dry seeds 100g (M0 generation), took them to Hainan Ling water soaking seeds to accelerate germination on the 2nd day after irradiation, sowed them at the Nanfan Experimental Station of Shanghai Academy of Agricultural Sciences on December 10, and cultivated strong seedlings by sparse sowing. Mixed transplanting and planting (M1) on January 8, 2012. After the M1 population matured, 1200 plant...
Embodiment 2
[0031] Shenwu 1A is a BT-type early-maturing and late-japonica backbone male sterile line bred by the Crop Breeding and Cultivation Research Institute of Shanghai Academy of Agricultural Sciences. It has several new combinations of high-quality, high-yield, and disease-resistant hybrid japonica rice. In the winter of 2016, the 350Gy 60 Coγ-rays irradiated the dry seeds of the main maintainer line Shenwu 1B of hybrid japonica rice. Using the method in Example 1, the stable mutant lines Y90 and Y100 were bred from the M4 generation in the summer of 2018 to form the main maintainer line Shenwu 1B of hybrid japonica rice. The heading stage bank of Wu 1B, with Shenwu 1A as the sterile cytoplasmic donor, and Y90 and Y100 as the nuclear donors, was used to simultaneously transfer sterile lines, and backcross for 2 generations, and a new sterile line was bred in the summer of 2019 Y90A and Y100A, together with Shenwu 1A, constituted the heading stage pool of Shenwu 1A, the main male s...
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