Efficient japonica hybrid rice selective-breeding method capable of reducing seed production cost

A low-cost hybrid japonica rice technology, applied in botany equipment and methods, application, plant gene improvement, etc., can solve the problems of high seed cost and low seed production yield, achieve efficient breeding, high mutation frequency, and improve seed production yield effect

Inactive Publication Date: 2020-08-04
SHANGHAI ACAD OF AGRI SCI +1
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Problems solved by technology

[0005] Aiming at the problems of low yield and high seed cost of hybrid japonica rice combined seed production, the present invention develops a high-efficiency breeding method for hybrid japonica rice that reduces the cost of seed production. The method utilizes 350Gy 60 The backbone parents of hybrid japonica rice were treated with Coγ-ray irradiation, combined with genetic background molecular marker selection and pressuri

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  • Efficient japonica hybrid rice selective-breeding method capable of reducing seed production cost

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Embodiment 1

[0024] Shenfan 24 is a high-quality and strong dominant hybrid japonica restorer line bred by the Institute of Crop Breeding and Cultivation of Shanghai Academy of Agricultural Sciences. , high-yield, and disease-resistant hybrid japonica rice combination, among which Shen 01A / Shen Fan 24 passed the Shanghai variety approval (named 'Shen You 24', approval number: Shanghai Approved Rice 2015001), but Shen Fan 24 has a long growth period The problem. To solve this problem, in the winter of 2011, Shanghai Shuneng Irradiation Technology Co., Ltd. used 350Gy 60 Coγ-rays irradiated Shenfan 24 dry seeds 100g (M0 generation), took them to Hainan Ling water soaking seeds to accelerate germination on the 2nd day after irradiation, sowed them at the Nanfan Experimental Station of Shanghai Academy of Agricultural Sciences on December 10, and cultivated strong seedlings by sparse sowing. Mixed transplanting and planting (M1) on January 8, 2012. After the M1 population matured, 1200 plant...

Embodiment 2

[0031] Shenwu 1A is a BT-type early-maturing and late-japonica backbone male sterile line bred by the Crop Breeding and Cultivation Research Institute of Shanghai Academy of Agricultural Sciences. It has several new combinations of high-quality, high-yield, and disease-resistant hybrid japonica rice. In the winter of 2016, the 350Gy 60 Coγ-rays irradiated the dry seeds of the main maintainer line Shenwu 1B of hybrid japonica rice. Using the method in Example 1, the stable mutant lines Y90 and Y100 were bred from the M4 generation in the summer of 2018 to form the main maintainer line Shenwu 1B of hybrid japonica rice. The heading stage bank of Wu 1B, with Shenwu 1A as the sterile cytoplasmic donor, and Y90 and Y100 as the nuclear donors, was used to simultaneously transfer sterile lines, and backcross for 2 generations, and a new sterile line was bred in the summer of 2019 Y90A and Y100A, together with Shenwu 1A, constituted the heading stage pool of Shenwu 1A, the main male s...

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Abstract

The invention relates to an efficient japonica hybrid rice selective-breeding method capable of reducing seed production cost. The method comprises the steps of irradiating a bone restorer line and amaintainer line of japonica hybrid rice with 350Gy of 60Co gamma-rays, carrying out parcel planting, carrying out genetic background selecting on a mutated line of a heading stage by using 48 SSR markers, constructing heading stage libraries of the bone restorer line and the maintainer line of the japonica hybrid rice with regard to pressurized screening of major breeding characters, and subjecting the maintainer line and a corresponding BT japonica rice sterile line to backcross transferring, so as to establish a bone sterile line library of the japonica hybrid rice. Through matched screeningof a test-cross sterile line/restorer line and a restorer line/sterile line close to a heading stage in the heading stage library, a high-quality high-yield disease-resistant japonica hybrid rice newcombination is selectively bred. According to the method, the efficiency of library establishing is high, the heading stage library of the bone restorer line can be established generally in 3 to 4 generations, the heading stage library of the bone sterile line can be established generally in 5 to 6 generations, and library establishing parents reserve major characteristics and excellent combiningability of original parents.

Description

technical field [0001] The invention relates to the technical field of crop breeding, in particular to a high-efficiency breeding method for hybrid japonica rice which reduces the cost of seed production. Background technique [0002] Rice is one of the most important food crops in the world, providing food for more than half of the world's population. At the same time, rice is also the largest grain crop in my country, and rice is the staple food for the survival of our people. The research and application of hybrid rice has greatly improved my country's rice production capacity and made outstanding contributions to ensuring my country's food security. With the improvement of people's living standards, the consumption demand for japonica rice is increasing day by day. The development of hybrid japonica rice has the double effect of improving the national grain output and the quality of life of the whole people. However, compared with hybrid indica rice, the planting ratio...

Claims

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Application Information

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IPC IPC(8): A01H1/06A01H1/02A01H1/04
CPCA01H1/06A01H1/02A01H1/04
Inventor 牛付安曹黎明程灿储黄伟周继华孙滨
Owner SHANGHAI ACAD OF AGRI SCI
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