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Three-step method for purification of Dian-1 type japonica sterile line stock seed

The technology of japonica rice and original seed is applied in the fields of botanical equipment and methods, plant genetic improvement, angiosperm/flowering plants, etc., and can solve the problems of inability to effectively prevent miscellaneous and pure, restricting the development of hybrid japonica rice, and low seed purity. To achieve the effect of shortening the production period of seeds, shortening the production period and reducing the number of reproductive generations

Inactive Publication Date: 2007-07-11
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional method of purifying the original CMS line of Dian I type japonica rice is to adopt the method of "single plant pairing and continuous backcrossing to reproduce the CMS line". It takes five generations of backcrossing to produce a large number of seeds, which takes a long time and cannot effectively prevent miscellaneous plants. The purity of the purified seeds is not high, which seriously restricts the development of hybrid japonica rice
At present, there is not a kind of technical perfection, which can effectively improve the purification method of the original seed purity of Dian I type japonica rice

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The "three-step method" is used for purification, that is, selecting a single plant, identifying the strain, and propagating the original species. In the backcross nursery where one grain seedling is planted in strict isolation, at the booting stage of the sterile line and the maintainer line, first select several individual plants of the sterile line and the maintainer line according to the typical characteristics of the original variety, and mark them in the field, and list the number . At the earing stage of the sterile line, according to the typical characteristics of the sterile line, select an excellent single plant of the sterile line for microscopic examination of pollen, count the number of dyed, round, and dead, and keep the same number of pollen as the sterile line Department of single plant. According to the results of pollen microscopic examination, 50 single plants of the sterile line with a sterility of 100% and 50 single plants of the maintainer line wi...

Embodiment 2

[0022] The implementation method is the same as Example 1. 100 individual plants of the sterile line with a sterility of 100% and 100 individual plants of the maintainer line with a normal pollen rate of more than 90% are selected for backcrossing in pairs, and these sterile lines are used at the same time. The test cross was carried out with the restorer line, and the seeds were harvested in pairs for planting identification and expansion of hand breeding in the following year. The method of "single-plant pairing and continuous backcross breeding of sterile lines" was used as a control, and the original seeds of the propagation were verified by field planting purity, and the results are shown in Table 2.

[0023] Table 2 Three-step method to purify the CMS original seed field planting purity identification results

[0024]

Embodiment 3

[0026] The implementation method is the same as Example 1, and 150 single plants of the sterile line with a sterility of 100% and 150 single plants of the maintainer line with a normal pollen rate of more than 90% are selected for backcrossing in pairs, and these sterile lines are used at the same time The test cross was carried out with the restorer line, and the seeds were harvested in pairs for planting identification and expansion of hand breeding in the following year. The method of "single-plant pairing and continuous backcross breeding of sterile lines" was used as a control, and the original seeds of the propagation were verified by field planting purity, and the results are shown in Table 3.

[0027] Table 3 Three-step method to purify the CMS original seed field planting purity identification results

[0028]

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PUM

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Abstract

The invention relates to a three-step method of I-type sterility rice, wherein it comprises that: selecting plant, checking, and cultivating. The invention can improve seed purity, hold former characters and shorten cultivate time.

Description

technical field [0001] The invention relates to a "three-step method" for purifying the original seed of Dian I type japonica sterile line, which belongs to the field of agriculture, more specifically, belongs to the field of seed production. Background technique [0002] Parental purification is an effective way to increase yield and income of hybrid rice. CMS is the basis of hybrid rice, and the purity of CMS is directly related to the purity of hybrid seeds, the full play of heterosis and the service life of hybrid combinations in production. Since the popularization of hybrid rice in our country, various places have attached great importance to the purification of sterile lines and the prevention of impurities. The current purification methods mainly include the line cycle method, the four-level seed production procedure, and the method of one selection and three nurseries. [0003] The Dian I-type CMS is one of the earliest rice CMSs used in my country today. Numerous ...

Claims

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Application Information

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IPC IPC(8): A01H1/00A01H1/04A01H1/02A01H5/10
Inventor 谭学林黄大军张忠林洪汝科金寿林陶光喜谭亚玲许红云顾晓明张树华
Owner YUNNAN AGRICULTURAL UNIVERSITY
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