The present invention discloses hippocampi wine and a method for preparing the same. The formula comprises the following raw materials of: fresh alive hippocampi, radix codonopsitis, astragalus, black date, Chinese wolfberry, eucommia, prepared radix rehmanniae, topaz, polygonatum sibiricum red, cistanche salsa, radix morindae, ganoderma lucidum, black bean, American ginseng, Korean Ginseng, pericarpium citri reticulataw, honey, caramel, and rice wine, etc. By adopting a unique process, the wine is made from natural compositions of the fresh alive hippocampi and a traditional Chinese medicine formula of 'medicine and food are from the same source'. The wine has the advantages of Qi invigorating, blood enriching, kidney tonifying, Yang strengthening, senescence postponing and good taste, etc.