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Method for improving quality of erect panicle japonica rice and molecular marker

A molecular marker and germplasm technology, applied in the fields of plant genetic improvement, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problems of poor rice quality, time-consuming and labor-intensive breeding methods, etc. Efficient process and stable effect

Inactive Publication Date: 2020-06-02
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Objective: In order to solve the deficiencies in the prior art, the present invention provides a method and molecular markers for improving the quality of erect panicle type japonica rice, so as to improve the poor rice quality of erect panicle type japonica rice varieties, and the time-consuming and labor-intensive breeding methods for rice quality improvement and other technical problems

Method used

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  • Method for improving quality of erect panicle japonica rice and molecular marker
  • Method for improving quality of erect panicle japonica rice and molecular marker
  • Method for improving quality of erect panicle japonica rice and molecular marker

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Embodiment 1

[0033] Example 1: A method for improving the quality of erect panicle type japonica rice

[0034] like figure 1 As shown, a molecular marker-assisted breeding method for improving the quality of erect panicle type japonica rice provided by the embodiments of the present invention includes:

[0035] S1: F 1 Hybrid;

[0036] S2: Select the target genotype is heterozygous and has excellent agronomic traits F 1 A single plant was backcrossed with Nanjing 46 to obtain BC 1 f 1 Hybrid;

[0037] S3 selects BC whose target genotype is heterozygous and has excellent agronomic traits 1 f 1 BC obtained by backcrossing a single plant with Nanjing 46 2 f 1 Hybrid;

[0038] S4: Select BC whose target genotype is heterozygous and has excellent agronomic traits 2 f 1 A single plant was backcrossed with Nanjing 46 to obtain BC 3 f 1 Hybrid;

[0039] S5: Select BC whose target genotype is heterozygous and has excellent agronomic traits 3 f 1 A single plant was backcrossed with ...

Embodiment 2

[0046] A molecular marker and PCR electrophoresis detection and analysis method for quality improvement of erect panicle type japonica rice:

[0047] (1) DNA extraction

[0048] DNA was extracted from rice leaves by CTAB method.

[0049] (2) Primer design

[0050] Utilize dCAPS software to design primers, the nucleotide sequence of described labeled primers is:

[0051] Forward primer G1-F: 5'-GGACGCAAGAACAGCC-3';

[0052] Reverse primer G1-R: 5'-TTCCACCATAGAAATCAACC-3'

[0053] (3) PCR amplification system:

[0054] Rice genomic DNA 20ng / ul 1.0ul, 10×PCR Buffer 2.0ul, 25mM MgCl 2 2.0ul, 2mMdNTP 2.0ul, 10uM primer 2.0ul, 5U / ul Taq DNA polymerase 0.2ul, ddH 2 O 10.8ul, total system 20ul.

[0055] (4) PCR reaction program:

[0056] Pre-denaturation at 95°C for 5 minutes; denaturation at 94°C for 1 minute, annealing at 55°C for 1 minute, extension at 72°C for 1 minute, 35 cycles; extension at 72°C for 10 minutes.

[0057] (5) Enzyme digestion system:

[0058] 10×Buffer...

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Abstract

The invention discloses a method for improving quality of erect panicle japonica rice and a molecular marker. The method comprises the following steps: identifying and screening low grain protein content QTL for rice germplasm resources to determine target rice variety resources; performing molecular marker-assisted selection on erect panicle japonica rice varieties to be improved according to thebreeding target, to obtain new japonica rice materials with excellent quality. The low grain protein content QTL adopts tightly linked molecular marker G1. The method can quickly and accurately obtain the new rice materials with excellent quality, has clear target and short breeding period, and is cost-saving, and the breeding process is more efficient.

Description

technical field [0001] The invention belongs to the technical field of rice breeding, and in particular relates to a method for improving the quality of erect panicle type japonica rice and a molecular marker. Background technique [0002] According to statistics, the world's population will exceed 9 billion by 2050. The world's population is exploding, the arable land is decreasing, and the global climate is changing rapidly. The world's food security is facing unprecedented challenges. Rice (Oryza sativa L.) is one of the most important food crops in China and even in the world, and plays a decisive role in ensuring food security. In the past ten years, the promotion and application of erect and semi-erect panicle varieties has brought the yield of japonica rice to a new level in my country, especially for the Northeast rice region of China. However, the rice quality of erect panicle varieties is generally considered to be poor, especially in terms of appearance quality a...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/6895A01H1/02
CPCA01H1/02C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 严长杰杨宜豪郭旻孙生远
Owner YANGZHOU UNIV
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