A kind of molecular breeding method of reproducible heterozygous female sterile restorer line and its application

A restorer line, female technology, applied in the field of genetic crop molecular biology

Active Publication Date: 2021-08-10
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI +1
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Problems solved by technology

However, the female sterile lines reported so far all reproduce sexually by themselves, so this method is difficult to popularize and apply

Method used

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  • A kind of molecular breeding method of reproducible heterozygous female sterile restorer line and its application
  • A kind of molecular breeding method of reproducible heterozygous female sterile restorer line and its application
  • A kind of molecular breeding method of reproducible heterozygous female sterile restorer line and its application

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Embodiment

[0024] Indica-japonica hybrid sterility is an important form of reproductive isolation in rice. At present, there are many indica-japonica hybrid sterility loci reported, and S5 is one of the main loci. There are three functional alleles at this locus, S5i is the allele of indica rice, S5j is the allele of japonica rice, and S5n is the intermediate type, that is, the broad affinity allele. The F1 females crossed with S5i allele and S5j allele are sterile (embryo sac sterile), but the pollen develops normally, and S5i and S5j allele and S5n can bear fruit normally. According to the functional characteristics of the different alleles of the above-mentioned S5 locus, the present invention utilizes the cloned thermosensitive genic sterility gene tms5 (Zhou et al., 2012) or the reflective photosensitive genic male sterile gene csa (Zhang et al., 2013), through hybridization Backcrossing and molecular marker selection means to create reproducible heterozygous female sterile restore...

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Abstract

The invention belongs to the technical field of molecular biology of genetic crops, and in particular relates to a molecular breeding method of a reproducible heterozygous female sterile restorer line and its application. In the present invention, the wide-compatibility gene S5n is used as a bridge to select and breed the japonica rice carrying S5i or the indica rice carrying S5j near-equal restorer lines; the temperature-sensitive sterile gene or the photosensitive sterility gene is introduced into the near-equal line of the S5i or S5j locus In the gene restorer line, a near-isogenic restorer line carrying S5i+ temperature-sensitive sterility gene or photo-reflex sterility gene, or S5j+ temperature-sensitive sterility gene or photo-reflex sterility gene is bred; through high temperature or short-day conditions, the seed production method , to realize the reproduction of heterozygous female sterile hybrid rice restorer lines. Since the restorer line selected by the present invention is female sterile but male fertile, it is not necessary to remove the male parent after pollination for seed production, realize mechanized harvesting for seed production, improve hybrid rice seed production efficiency, and provide technical support for hybrid rice mechanized seed production.

Description

technical field [0001] The invention belongs to the technical field of molecular biology of genetic crops, and in particular relates to a molecular breeding method of a reproducible heterozygous female sterile restorer line and its application. Background technique [0002] The invention, promotion and application of hybrid rice have made significant contributions to food security in my country and the world. However, hybrid rice seed production has many links and complicated procedures, and relies on intensive labor, which has high cost and low efficiency. With the continuous expansion of the scale of urbanization in my country, the rural labor force population has decreased sharply. Therefore, it is particularly urgent to research and establish hybrid rice seed production technology with full mechanized operation. [0003] At present, in the process of hybrid rice seed production, the removal of restorer lines after pollination is an important factor affecting the mechan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/04A01H1/02
CPCA01H1/02A01H1/04
Inventor 倪金龙周海倪大虎宋丰顺张德文杨剑波钱征林翠香
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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