Molecular marker of low-temperature-sensitive genic male sterile gene and application thereof

Abstract

The invention belongs to the technical fields of crop genetics and breeding, and particularly relates to a molecular marker of a low-temperature-sensitive genic male sterile gene and application thereof. According to the molecular marker of the low-temperature-sensitive genic male sterile gene, a reverse-temperature-sensitive genic male sterile resource, namely a Yannong-S low-temperature-sensitive genic male sterile gene rtms10, is located on chromosome 10 of rice through genetic analysis; and a related molecular marker ID1305 is developed. Then, a fertile parent is selected as a recurrent group, and is cross-bred, back-crossed and self-bred with the Yannong-S low-temperature-sensitive genic male sterile gene rtms10; and a reverse-temperature-sensitive genic male sterile line is selectedand bred by utilizing a molecular marker selection means. Amplified fragments of the developed molecular marker ID1305 in the low-temperature-sensitive genic male sterile gene rtms10 is 159 bp; and the amplified fragments of the developed molecular marker ID1305 in normal fertile materials is 180 bp. On basis of differences of amplification products in the reverse-temperature-sensitive genic malesterile line and the normal materials, the molecular marker ID1305 can be used for identifying purity of two-line hybrid rice combined with the reverse-temperature-sensitive genic male sterile line, as well as for molecule selecting and breeding of the reverse-temperature-sensitive genic male sterile line.

Description

technical field [0001] The invention belongs to the technical field of crop genetic breeding, and in particular relates to a molecular marker of a low-temperature-sensitive sterility gene and an application thereof. Background technique [0002] The discovery of rice light- and temperature-sensitive sterile resources (two-line sterile resources) and its successful application in hybrid rice have played an important role in ensuring food security in my country. Compared with the three-line CMS, the two-line CMS has the advantages of self-propagation and wide recovery spectrum. Therefore, the two-line hybrid rice has developed rapidly in my country in recent years, and the planting area and promotion range have expanded rapidly. However, the promotion of two-line hybrid rice also faces many difficulties such as high risk of seed production, narrow breeding area of ​​sterile lines, and single type of practical sterile resources. [0003] The discovery of light- and temperature...

AI Technical Summary

Problems solved by technology

Among these GMS materials, since most of the fertility conversion critical temperatures are between 22 and 25°C (Cai Yidong, 2003), the risk of seed production is extremely high. It is difficult to guarantee that there will be no weather with an average daily temperature higher than 25°C, so it is not practical

Images