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Novel method for constructing rice sporinite-gametophyte dual sterile line

A gametophyte and sporophyte technology is applied in the field of constructing a rice sporophyte-gametophyte double sterile line, and achieves the effects of reducing the risk of seed production, thorough fertility abortion and improving safety.

Inactive Publication Date: 2009-02-11
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Examples

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Embodiment 1

[0018] Example 1: The light- and temperature-sensitive genic male sterile line Xiang 125S (sporophytic sterile) was used as the female parent, and the maintainer line Yuetai B of the gametophytic cytoplasmic sterile line Yuetai A was used as the male parent, and F1 and F1 were obtained by crossing Self-cross to get F2, select photo-thermo-sensitive sterile plants from F2 and backcross with Yuetai B to get BC1, self-cross BC1 to get BC1F2, select photo-thermo-sensitive sterile plants from BC1F2 and back-cross with Yuetai B to get BC2, BC2 Self-cross to obtain BC2F2, select photothermo-sensitive sterile single plants in BC2F2 and backcross with Yuetai B to obtain BC3,..., repeat the above-mentioned process of "self-cross-back-cross-self-backcross", after 4 rounds of backcross , to obtain BC4, BC4 was self-crossed to obtain BC4F2, and the photothermosensitive sterile single plant selected in BC4F2 was a two-line male sterile line with gametophytic sterile gene, which was recorded ...

Embodiment 2

[0019] Example 2: Using the light- and temperature-sensitive GMMS strain 1S (sporophytic sterile) as the female parent, and the maintainer line Congguang 41B of the gametophytic cytoplasmic sterile line Congguang 41A as the male parent, F1 and F1 were obtained by crossing Self-cross to get F2, choose photothermo-sensitive sterile single plant from F2 and backcross with Congguang 41B to get BC1, self-cross BC1 to get BC1F2, choose photothermo-sensitive sterile single plant from BC1F2 to backcross with Congguang 41B to get BC2, BC2 Self-cross to get BC2F2, select photothermo-sensitive sterile single plants in BC2F2 and backcross with Congguang 41B to get BC3,..., repeat the above process of "self-cross-back-cross-self-backcross", after 8 rounds of backcross , to obtain BC8, BC8 was self-crossed to obtain BC8F2, and the photothermo-sensitive sterile single plant selected in BC8F2 was a two-line male sterile line with gametophytic sterile gene, which was recorded as Congguang 41Bs....

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Abstract

The invention relates to a method for constructing rice sporinite-gametocyte sterile system, wherein it uses the light-temperature sensitive sterile system of sporinite and the cytoplasm sterile system of gametocyte, to obtain the sterile system with sporinite sterile genes and gametocyte sterile gene. The invention can improve the safety of cross rice seed preparation.

Description

technical field [0001] The invention relates to a method for cultivating hybrid rice, in particular to a new method for constructing a rice sporophyte-gametophyte double sterile line. Background technique [0002] Plant male sterility is a genetic phenomenon in which plants cannot produce normal anthers, pollen or male gametes during sexual reproduction, and it widely exists in flowering plants. Rice is a self-pollinating crop, and its reproduction is accomplished by pollinating the female gametes with its own pollen. However, in the production of hybrid rice, it is necessary to mate with parents with relatively large genetic differences (that is, not self-pollination) to obtain hybrids with strong advantages (high yield). Therefore, effective measures must be taken to prevent self-pollination during seed production. The discovery and utilization of male sterile materials provide an effective technical guarantee for the utilization of rice heterosis. Because it can effect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 陈建国周勇居超明徐国成
Owner HUBEI UNIV
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