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Reproducible heterozygous female sterile restorer molecular breeding method and application thereof

A restorer line, female technology, applied in the field of genetic crop molecular biology

Active Publication Date: 2020-02-18
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI +1
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the female sterile lines reported so far all reproduce sexually by themselves, so this method is difficult to popularize and apply

Method used

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  • Reproducible heterozygous female sterile restorer molecular breeding method and application thereof
  • Reproducible heterozygous female sterile restorer molecular breeding method and application thereof
  • Reproducible heterozygous female sterile restorer molecular breeding method and application thereof

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Embodiment

[0024] Indica-japonica hybrid sterility is an important form of reproductive isolation in rice. At present, there are many indica-japonica hybrid sterility loci reported, and S5 is one of the main loci. There are three functional alleles at this locus, S5i is the allele of indica rice, S5j is the allele of japonica rice, and S5n is the intermediate type, that is, the broad affinity allele. The F1 females crossed with S5i allele and S5j allele are sterile (embryo sac sterile), but the pollen develops normally, and S5i and S5j allele and S5n can bear fruit normally. According to the functional characteristics of the different alleles of the above-mentioned S5 locus, the present invention utilizes the cloned thermosensitive genic sterility gene tms5 (Zhou et al., 2012) or the reflective photosensitive genic male sterile gene csa (Zhang et al., 2013), through hybridization Backcrossing and molecular marker selection means to create reproducible heterozygous female sterile restore...

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Abstract

The invention belongs to the technical field of genetic crop molecular biology and particularly relates to a reproducible heterozygous female sterile restorer molecular breeding method and applicationthereof. The method includes: bridging by a wide compatibility gene S5n to breed near-isogenic restorer lines of S5i-carrying japonica rice or S5j-carrying indica rice; introducing a temperature-sensitive sterile gene or an anti-photosensitive genic sterile gene into near-isogenic gene restorer lines of an S5i or S5j locus to breed near-isogenic restorer lines with S5i+ temperature-sensitive sterile genes or anti-photosensitive sterile genes, or S5j+ temperature-sensitive sterile genes or anti-photosensitive sterile genes; by seed production under high-temperature or short-day conditions, breeding of heterozygous female sterile hybrid rice restorers is realized. Since females are sterile while males are fertile in the bred restorers, male parent removal after pollination in seed production is avoided, seed-production mechanical harvesting is realized, hybrid rice seed production efficiency is improved, and technical guarantee is provided for mechanical seed production of hybrid rice.

Description

technical field [0001] The invention belongs to the technical field of molecular biology of genetic crops, and in particular relates to a molecular breeding method of a reproducible heterozygous female sterile restorer line and its application. Background technique [0002] The invention, promotion and application of hybrid rice have made significant contributions to food security in my country and the world. However, hybrid rice seed production has many links and complicated procedures, and relies on intensive labor, which has high cost and low efficiency. With the continuous expansion of the scale of urbanization in my country, the rural labor force population has decreased sharply. Therefore, it is particularly urgent to research and establish hybrid rice seed production technology with full mechanized operation. [0003] At present, in the process of hybrid rice seed production, the removal of restorer lines after pollination is an important factor affecting the mechan...

Claims

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Application Information

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IPC IPC(8): A01H1/04A01H1/02
CPCA01H1/02A01H1/04
Inventor 倪金龙周海倪大虎宋丰顺张德文杨剑波钱征林翠香
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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