A method for editing rice ehd1 gene to breed long-growing japonica rice varieties

Abstract

The invention provides a method for cultivating "Northern Japonica to Southward" rice varieties, which includes the following steps: Ehd1 Design the CRISPR / Cas9 gene editing site in the starting region of the gene receiving domain and construct the gene editing vector to transform rice to achieve Ehd1 Site-directed mutagenesis of genes, obtained Ehd1 Gene frameshift mutation and in-frame deletion mutant strain in which 3 amino acids are deleted from the start region of the receiving domain. Experiments have shown that compared with the wild type, the growth period of the in-frame deletion mutant strain is moderately extended by about two weeks, and the growth period of the frameshift mutant is delayed by nearly one month. Through the method provided by the invention, japonica rice materials with different growth periods extended can be quickly obtained, and the ecological adaptability of existing excellent varieties can be expanded. It can provide an efficient gene knockout method and breeding method for the selection of japonica rice varieties that can adapt to different ecological conditions in low latitude areas.

Description

technical field [0001] The invention relates to the field of plant transgenic technology and crop genetic breeding, and relates to a method of editing rice using CRISPR-Cas9 technology Ehd1 The gene receiving domain prolongs the growth period of rice, and the breeding method of cultivating "Northern Japonica southward migration" rice varieties. Background technique [0002] Rice is the main food crop and the staple food of nearly half of the world's population. Rice is divided into two subspecies, indica and japonica. Among them, japonica rice is of high quality and its market price is generally higher than that of indica rice. Japonica rice is very popular in many countries. In order to meet the market consumption demand, the Chinese government has put forward the "indica rice to japonica rice" project focusing on improving the quality of rice. [0003] The main limiting factor affecting the introduction of northern japonica rice varieties to low-latitude areas is that the...

AI Technical Summary

Problems solved by technology

At present, conventional hybrid breeding is the main rice breeding method, and traditional techniques can be used to hybridize and backcross ehd1 Mutant genes are transferred into superior rice varieties to breed japonica rice varieties suitable for growth and growth in low-latitude regions. However, the main disadvantages of these breeding methods are labor-intensive, high-cost and long-breeding cycles.

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