Method for rapidly and accurately breeding semi-glutinous japonica rice restorer line

Abstract

The invention belongs to the technical field of rice genetic breeding, and discloses a method for rapidly and accurately breeding a semi-glutinous japonica rice restorer line. The method comprises the following steps that semi-glutinous conventional japonica rice Nanjing 9108 which contains a semi-glutinous gene wxmq but does not contain a male sterility fertility restorer gene Rfla is selected as a donor parent and a non-semi-glutinous japonica rice restorer line Ninghui 821 containing an Rfla gene is selected as a receptor parent, backcross and selfing are utilized, meanwhile, molecular markers are used for auxiliary selection, and semi-glutinous genes and male sterility fertility restorer genes are polymerized into the same variety to obtain the semi-glutinous japonica rice restorer line. According to the method, screening is conducted through a specific molecular marker rapid detection method, the breeding efficiency is improved through rapid, accurate and efficient transformation and molecular-assisted selection, effective polymerization of the semi-glutinous genes and restorer line genes is achieved in the japonica rice restorer line, and rapid application of semi-glutinous hybrid japonica rice in production is accelerated while a breeding process is accelerated.

Description

technical field [0001] The invention belongs to the technical field of rice genetics and breeding, and in particular relates to a method for rapid and precise selection of semi-waxy japonica restorer lines. Background technique [0002] At present, hybrid japonica rice has been in a low ebb state after experiencing the glory in the 1980s. Although a series of new hybrid japonica rice combinations have been bred, the area of ​​promotion in production is not large, and it is difficult to compete with conventional japonica rice. The japonica rice area is still dominated by conventional varieties. The situation of the world. At the same time, early breeders did not pay enough attention to the rice quality of hybrid rice. Compared with conventional rice, the rice quality of hybrid japonica rice generally deviates. The prior art 1 analysis of 56 state-approved hybrid japonica rice and conventional japonica rice shows that although the hybrid japonica rice is not much different fr...

AI Technical Summary

Problems solved by technology

Conventional breeding methods for semi-waxy japonica rice restorer lines take a long time to breed and have low selection efficiency for target traits

[0005] Through the above analysis, the existing problems and defects of the existing technology are: the existing methods for breeding semi-waxy japonica restorer lines take a long time for breeding and have low selection efficiency for target traits

[0006] The difficulty of solving the above problems and defects is: when conventional breeding methods solve the above technical problems, it often takes a long time period and a large amount of data detection and rice appearance quality appraisal, etc., and it is necessary to wait until the target traits of the breeding target material are stabilized. To identify and test

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