Method for assistantly screening indica-type rice and japonica rice, and special primer therefor

Abstract

The invention discloses a filtration assisting method of Indica and Japonica and a specific primer thereof. The filtration assisting method detects whether segments shown in (a) and (b) are deleted in a protein gene that is shown in sequence 4 of an encoding sequence table in a genome of rice to be detected, and if so, the rice to be detected is Indica candidate, otherwise the rice to be detected is Japonica candidate; wherein, (a) is a DNA segment of 717 to 855 nucleotide from 5'-end in the sequence 3 of the sequence table; and (b) is a DNA segment in a nucleotide sequence that has the homology degree of over 97 percent with sequences that are limited by (a). The filtration assisting method of Indica and Japonica subtypes has important guidance in filtering Indica and Japonica varieties or local varieties in an early development stage, acknowledging the genetic differentiation degree between Indica and Japonica, utilizing the advantages of hybrid varieties Indica and Japonica, and culturing high-yield and high-quality hybrid rice, and can consequently optimize hybrid combinations, quicken breeding speed and lower breeding cost, has the advantages of simple operation, low cost and short period, fits for popularization and application, and provides a fast selection method for the quality identification of rice subtypes Indica and Japonica.

Description

technical field [0001] The invention relates to a method for assisting in screening indica rice and japonica rice and special primers thereof. Background technique [0002] Cultivated rice is one of the most important food crops in the world. It is the main source of heat for more than one-third of the world's population. The improvement of its yield and quality is crucial to the national economy and people's livelihood, social stability and food security. Cultivated rice has a domestication history of more than 11,500 years, and two different ecological types of indica rice and japonica rice have been produced to adapt to different agricultural ecological environments (Morishima et al, Japanese Journal of Breeding, 1981, 31: 402-413; Glaszmann et al, Theoretical and Applied Genetics, 1987, 74:21-30; Blair et al, Theoretical and Applied Genetics, 1999, 98:780-782). During the long evolutionary process, indica and japonica rice have obvious differences in many morphological ...

AI Technical Summary

Problems solved by technology

However, due to the complexity of the growth environment, during the long process of indica / japonica rice differentiation, not only typical indica or japonica rice, but also intermediate types with indistinct differentiation between indica and japonica rice were produced. It is difficult to accurately identify through traditional methods, which brings difficulties to the study of the origin and evolution of rice, and also hinders the cultivation of new rice varieties

It also increases the difficulty of breeding new rice varieties

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