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Method for detecting number of littermate and characters of sow

A technology of litter size and traits, which is applied in the field of detection of litter size traits, and can solve problems such as low heritability and difficulty in improving litter size traits

Inactive Publication Date: 2011-01-26
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The number of piglets born alive is one of the main indicators for evaluating the reproductive performance of pigs. It is a quantitative trait with low heritability, and it is difficult to improve the trait of piglets born alive by conventional breeding techniques.

Method used

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  • Method for detecting number of littermate and characters of sow
  • Method for detecting number of littermate and characters of sow
  • Method for detecting number of littermate and characters of sow

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Embodiment 1, single nucleotide polymorphism analysis of LHβ gene

[0072] The blood or tissue samples of isolated experimental animals came from Jiangxi Provincial Pig Farm, Tianjin Changhe Pig Farm, Yantai Yisheng Pig Farm and Jiangsu Changshu Breeding Farm.

[0073] 1. Genomic DNA extraction:

[0074]Take 500 μl of lysed blood sample and add 350 μl of blood extract, mix well; add 4 μl of proteinase K (20mg / ml) to a final concentration of 200 μg / ml; after digestion at 50-55°C overnight, add 173ml of preheated 5M Nacl to a final concentration of 1.5M , mix well; extract with an equal volume of chloroform; take the supernatant and extract with saturated phenol:chloroform (1:1); add 2 times the volume of absolute ethanol to the aqueous phase. Then centrifuge, discard the supernatant, wash the precipitate with 70% ethanol, dry in vacuum or air after centrifugation, and dissolve the precipitate in sterile double distilled water.

[0075] 2. PCR-SSCP process:

[0076] (1...

Embodiment 2

[0094] Example 2, Correlation analysis between single nucleotide polymorphisms of pig LHβ subunit gene and reproductive traits

[0095] The number of piglets born alive is one of the main indicators for evaluating the reproductive performance of pigs. It refers to the number of piglets that can survive in each litter of sows. The recording method is to record the number of piglets that can survive after weaning.

[0096] The haplotype of individual pigs was determined according to the method in Example 1.

[0097] Record the number of live litters of multiparous pigs in Yantai Yisheng Breeding Farm and Jiangsu Changshu Fine Breeding Farm, detect the haplotype of each pig, and use the least square analysis method to compare the haplotype of pigs with the multiparous litter. The relationship between litter size traits was analyzed statistically. Statistical analysis model:

[0098] the y ijkl =μ+B i +HYS j +G k +e ijkl

[0099] where y ijkl is the observed value of the...

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Abstract

The invention discloses a method for detecting number of littermates and characters of a sow, which comprises the steps of: 1, detecting whether the 71th nucleotide from 5' end of a DNA segment shown in SEQ ID NO:1 in a genome of a sow to be detected is G or A; detecting whether the 81th nucleotide from 5' end of a DNA segment shown in SEQ ID NO:2 in the genome of the sow to be detected is C or A; and determining the haplotype of the sow to be detected, determining the number of the littermates and the characters of the sow to be detected according to the haplotype. The method can be used for detecting reproduction characters of the sow in a development early stage and knowing the genetic differentiation degree of the sow, provides a useful molecular marker for genotyping, screening and auxiliary breeding of different haplotypes of the sow, and has important meanings on improving the number of the littermates of the sow. Therefore, the method is beneficial to the quickening of the breeding speed and the reducing of the breeding cost, has the advantages of low cost, simple operation and short period, and is suitable for popularization and application.

Description

technical field [0001] The invention relates to a method for detecting the traits of piglets born alive. Background technique [0002] Luteinizing hormone (LH) is a glycoprotein secreted by pituitary basophils with a molecular weight of 28000D. LH, FSH, TSH, CG, etc. are all composed of α and β subunits through non-conjugated combination, which can be easily separated by different treatments. Among them, the α subunit is the same among the four hormones, and the β subunit determines the specific functions of various hormones. The role of LH in male animals is mainly to stimulate the Leydig cells to produce androgen, and through the androgen to affect the spermatogenic process of the seminiferous tubules. In female animals, LH is required for the synthesis of testosterone and estradiol in the ovary and also induces the synthesis of progesterone and progesterone receptors in granulosa cells. In the estrous cycle of adult animals or in the blood of menstrual cycle, the appea...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 李宁赵要风王爱华胡晓湘
Owner CHINA AGRI UNIV
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