Auxiliary screening method for indica rice and japonica rice

An indica rice and japonica rice technology, which is applied in the directions of biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., to achieve the effect of optimizing hybrid combinations, low cost, and quick selection methods

Inactive Publication Date: 2008-07-09
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report on the function of OsSRT2(LOC_Os12g07950)

Method used

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  • Auxiliary screening method for indica rice and japonica rice
  • Auxiliary screening method for indica rice and japonica rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. Discovery and verification of different expression patterns of gene LOC_Os12g07950 in indica and japonica rice

[0021] Firstly, by mining and analyzing the chip data of indica and japonica rice at different developmental stages and different tissue parts, it was found that the gene LOC_Os12g07950 had two sets of probes in the GengChip data, and the expression patterns of these two sets of probes in indica and japonica rice were opposite. , the expression level of probe P1 is very low or even not expressed in indica rice, but the expression level in japonica rice is very high, while the expression level of another probe P2 is very high in indica rice, but very low in japonica rice . According to previous research results, the main reason for the difference in gene expression may be the difference in base sequence (InDel), or it may be regulated by other genes. Therefore, using the method of comparative genomics, the genome sequence of gene LOC_Os12g07950 in ...

Embodiment 2

[0022] Embodiment 2, be used for the design of the primer of auxiliary screening indica rice and japonica rice

[0023] In order to further verify whether there is a genome-level difference between 93-11 and Nipponbare, according to the Nipponbare sequence, primers SIR2-2 were designed in the region where there may be genomic differences between the two. One of the primer sequences is shown in sequence 1 in the sequence table, and the other A primer sequence is shown as sequence 2 in the sequence table, and the genomic DNA of Nipponbare and 93-11 were respectively used as templates for PCR amplification verification. The reaction system of PCR amplification is: rice genomic DNA template 20ng, Taq Plus DNA polymerase 0.5U, 2.0μl 10×PCR buffer (100mM Tris HCl pH9.0, 500mM KCl, 15mM Mg 2+, 1% Triton X-100), 100 μM dNTPs, 4 μM forward primer, 4 μM reverse primer, supplement the reaction system with DEPC water to 20 μl. The PCR reaction conditions were as follows: first at 94°C fo...

Embodiment 3

[0025] Example 3. Feasibility verification of using primer SIR2-3 to assist in screening rice indica and japonica subspecies

[0026] Rice varieties and local species (both from the National Germplasm Bank): 93-11, Xima Zhagu, Zinuo, Nepa (deep water rice), Guichao 2, Aus6538 (Bamalaia), IR24, Teqing, Nipponbare, Ai Dazhong, Muli (japonica rice with light shell), glutinous japonica rice, Yuefu, Qiuguang, Hongguang and Yunfeng 7.

[0027] First, 8 varieties and landraces of each subspecies of indica and japonica were randomly selected by using morphological indicators and related literature retrieval, as shown in Table 1:

[0028] Table 1. Identification results of indica and japonica subspecies of 16 rice varieties and landraces

[0029] species name

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Abstract

The invention discloses a process of auxiliary screening hsien rice and japonica rice. The process for auxiliary screening hsien rice and japonica rice which is provided by the invention is used to detect whether rice losses ribonucleotide sequence of 67843-67878 from 5' end in GenBank accession AL731882, the detected rice is candidate hsien rice if lost, or the detected rice is candidate japonica rice if not lost. The process for auxiliary screening rice subspecies which is provided by the invention can be used to evaluate hsien rice and japonica rice breeds or local breed in early period, and plays important guiding roles for using heterosis of hsien rice and japonica rice and cultivating high-yield and high grade hybridization rice, thereby optimizing hybridized combination, accelerating breeding speed, reducing breeding cost, and having the advantages of simple operation, low cost and short cycle, which is suitable to be popularized and applied, and provides a fast choose method for the evaluation of rice subspecies germplasm.

Description

technical field [0001] The invention relates to a method for assisting selection of indica rice and japonica rice. Background technique [0002] Cultivated rice is one of the most important food crops in the world. During its domestication history of more than 11,500 years, in order to adapt to different agro-ecological environments, cultivated rice has formed rich genetic diversity and obvious genetic differentiation, resulting in indica ( indica) and japonica (Morishima H, Oka HI. Phylogenetic differentiation of cultivated rice. XXII. Numerable evaluation of the Indica-Japonica differentiation. Japanese Journal of Breeding, 1981, 31: 402-413; Glaszmann J C. Isozymes and classification of Asian rice varieties. Theoretical and Applied Genetics, 1987, 74: 21-30; Wang Z Y, Tanksley S D. Restriction fragment length polymorphism in Oryzasativa L. Genome, 1989, 32: 1113-1118; Blair M W, Panaud O, McCouch S R. Inter-simple sequence repeat (ISSR) amplification for analysis of micr...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 苏震刘凤霞徐文英谭禄宾邸超张振海孙传清薛勇彪
Owner CHINA AGRI UNIV
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