Method for assisting sifting motion of nonglutinous rice and non glutinous rice

A technology for indica and japonica rice, which is applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as difficult to study the degree of indica-japonica differentiation in rice, indica-japonica differentiation is not obvious, intermediate, slow, etc. , to achieve the effect of speeding up breeding, low cost and simple operation

Inactive Publication Date: 2008-07-16
CHINA AGRI UNIV
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, the indica-japonica differentiation of rice is a slow evolutionary process. There are typical indica or japonica varieties in many rice landraces, but there are also intermediate types with indistinct indica-japonica differentiation. It is difficult to use traditional methods for these types. identification, it is more difficult to study the degree of indica-japonica differentiation in rice

Method used

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  • Method for assisting sifting motion of nonglutinous rice and non glutinous rice
  • Method for assisting sifting motion of nonglutinous rice and non glutinous rice
  • Method for assisting sifting motion of nonglutinous rice and non glutinous rice

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Example 1. Discovery and verification of different expression patterns of gene LOC_Os06g48030 in indica and japonica subspecies

[0032]Through the mining and analysis of indica and japonica rice chip data at different developmental stages and different tissue parts, it was found that the gene LOC_Os06g48030 corresponds to three sets of probes in the GengChip data: Os.11547.1.S1_s_at(PS1), OsAffx.28164.1.S1_at(PS2) and OsAffx.28164.2.S1_at(PS3), in which the probe group PS1 has a higher expression level in indica and japonica rice, and there is no significant difference, while the expression pattern of the probe group PS2 and PS3 in indica and japonica rice is opposite, PS2 only It has a high expression level in indica rice and almost no expression in japonica rice, while PS3 has a very high expression level only in japonica rice tissue, while the expression level in indica rice is very low or even not expressed (Fig. 1, where A is The expression profile of Os.11547.1.S...

Embodiment 2

[0037] Example 2. Exploration of the mechanism of different expression patterns of gene LOC_Os06g48030 in indica and japonica subspecies and the design of primers for auxiliary screening of indica and japonica rice

[0038] In order to explore the mechanism of gene LOC_Os06g48030 different expression patterns in indica and japonica rice, the structure of this gene was analyzed. In view of the fact that this gene lacks the full-length cDNA sequence in indica rice, firstly, Primer3 software was used to design primers FS (the primer sequence of FS is FS_F: 5'-ATGGGGCAGAGGAGGAGGTC-3'; FS_R: 5'-CAAAAAGGAATGGCATATGTATGGGA-3'), and the indica rice 93 -11 and the cDNA of the japonica rice Nipponbare were used as templates for PCR amplification. As a result, only a band of about 1300 bp was detected in the indica rice 93-11 (Fig. 2D). Subsequently, the product was cloned and sequenced, and the sequence is shown in sequence 3 in the sequence table, and the measured sequence and the full...

Embodiment 3

[0043] Example 3. Feasibility verification of primer GS-assisted screening of rice indica and japonica subspecies

[0044] The rice varieties used for verification were all from the National Germplasm Bank: 93-11, IR24, 03A-11, 03A-9, Zhongyou 13, Nipponbare, Hua 1, 746, Yunfeng 7 and Xiangjingnuo.

[0045] Firstly, 8 varieties and landraces (4 indica and 4 japonica) were randomly selected using morphological indicators and related literature search, as shown in Table 2:

[0046] Table 2. Identification results of indica and japonica subspecies of 8 rice varieties and landraces

[0047] rice variety

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Abstract

The invention discloses an assisted screening method of indica type rice and japonica rice. The assisted screening method of indica type rice and japonica rice which is provided by the invention is to detect whether the 71046 to 72062 nucleotide sequence from the 5' tail end of GenBank accession AP003771 is deleted in rice to be measured, if deleted, the rice to be measured is the candidate indica type rice, if not deleted, the rice to be measured is the candidate japonica rice. The assisted screening method of indica type rice and japonica rice of the invention can be used for identifying the variety and the local races of the indica type rice and the japonica rice at an early stage and knowing the genetic differentiation degree of the indica type rice and the japonica rice, thus the invention has important guide function for utilizing the hybrid advantages of the indica type rice and the japonica rice and cultivating the high-yield and high-quality hybrid rice so as to optimize hybrid combination, accelerate breeding speed and reduce breeding cost; the invention has the advantages of simple operation, low cost and short period, which is applicable to popularization and application and provides a fast choosing method for the identification of the germplasm of the subspecies of the indica and japonica of the rice.

Description

technical field [0001] The invention relates to a method for assisting selection of indica rice and japonica rice. Background technique [0002] Cultivated rice is one of the most important food crops in the world. During its domestication history of more than 11,500 years, in order to adapt to different agro-ecological environments, cultivated rice has formed rich genetic diversity and obvious genetic differentiation, resulting in indica ( indica) and japonica (Morishima H, Oka HI. Phylogenetic differentiation of cultivated rice. XXII. Numerable evaluation of the Indica-Japonica differentiation. Japanese Journal of Breeding, 1981, 31: 402-413; GlaszmannJC. Isozymes and classification of Asian rice varieties. Theoretical and Applied Genetics, 1987, 74: 21-30; Wang Z Y, Tanksley S D. Restriction fragment length polymorphism in Oryzasativa L. Genome, 1989, 32: 1113-1118; Blair M W, Panaud O, McCouch S R. Inter-simple sequence repeat (ISSR) amplification for analysis of micros...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 苏震刘凤霞徐文英谭禄宾薛勇彪孙传清
Owner CHINA AGRI UNIV
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