11 results about "Sodium nitroprusside" patented technology

The invention discloses an underground propagule composite excitant for ecological restoration and a preparation method thereof, and relates to the technical field of excitant preparation. The fertilizer is prepared from the following raw materials in parts by weight: 0.2 to 0.3 part of gibberellin, 0.2 to 0.5 part of 6-benayl aminopurine, 0.2 to 0.3 part of indolebutyric acid, 0.5 to 0.7 part of sodium nitroprusside, 0.4 to 0.5 part of hydrogen peroxide, 1 to 2 parts of jasmonic acid sustained-release microspheres, 5 to 7 parts of compound amino acid, 1 to 3 parts of compound microbial agent sustained-release microspheres, 2 to 3 parts of seaweed extract, 10 to 13 parts of humic acid, 2 to 3 parts of monopotassium phosphate, 60 to 74 parts of ethanol and 1000 parts of water. The underground propagule composite excitant provided by the invention is beneficial to promoting activation and germination of underground propagules, enhancing plant growth and stress resistance, improving soil micro-ecological functions, forming stable diversity communities and facilitating ecological restoration.

This invention belongs to the pharmaceutical field, specifically relating to a sodium nitroprusside injection and its preparation method. The sodium nitroprusside injection comprises the following raw materials in parts by weight: 1.5–3.5 parts sodium nitroprusside and 100 parts water for injection. This invention solves the problem of poor stability of sodium nitroprusside aqueous solutions by optimizing the formulation and preparation process without introducing other excipients. The sodium nitroprusside injection prepared by this invention is a sterile injectable preparation manufactured using modern aseptic filling technology combined with non-terminal sterilization. It not only has the advantages of direct and rapid action and high sterility assurance, but also has few adverse reactions and can be transported and stored at room temperature, thus improving the stability and safety of the product. This invention also provides its preparation method, which is simple, safe to use, and has stable quality.

The present application relates to the technical field of nanomaterial application, and provides a ruthenium-doped Prussian blue nanoscale enzyme and a preparation method thereof, wherein the ruthenium-doped Prussian blue nanoscale enzyme is obtained by reacting metal ruthenium with Prussian blue nanoparticles under normal temperature conditions. The present application also provides a nanoscale enzyme library containing the ruthenium-doped Prussian blue nanoscale enzyme and a construction method thereof. The present application also provides SNP-PB nanoscale enzyme obtained by loading sodium nitroprusside into the ruthenium-doped Prussian blue nanoscale enzyme. The ruthenium-doped Prussian blue nanoscale enzyme and the SNP-PB nanoscale enzyme both have good antibacterial and anti-inflammatory properties, and can better solve the problem of biofilm resistance after being endowed with the ability to release NO gas, thereby better exerting antioxidant stress and fighting periodontal bacterial infection, and providing a new treatment method for periodontal disease.

This invention provides a sodium nitroprusside / Prussian blue / manganese composite nanozyme, its preparation method, and its application in antibacterial applications. Belonging to the field of nanomaterials technology, the preparation method involves: first, polyvinylpyrrolidone, potassium ferricyanide, and sodium nitroprusside are mixed in an acidic solution at room temperature to prepare a Prussian blue precursor solution; then, manganese acetate is added dropwise to the Prussian blue precursor solution; after high-pressure reaction, centrifugation, and freeze-drying, sodium nitroprusside / Prussian blue / manganese composite nanozyme powder is obtained. The sodium nitroprusside / Prussian blue / manganese composite nanozyme prepared by this invention possesses a synergistic antibacterial mechanism of "enzyme catalysis + photothermal / ultrasound-based kinetic therapy + NO release." Through dual regulation of NO release via laser or ultrasound, combined with photothermal and acoustic-dynamic effects, it disrupts bacterial structure and metabolism, exhibiting not only highly effective killing of Staphylococcus aureus but also clearing bacterial biofilms, effectively solving the problem of biofilm-mediated drug resistance and providing a new approach in the field of antibacterial research.

The present invention relates to the field of plant breeding and in particular to the regeneration of plants from cells and other tissues. More particularly, the invention provides methods and means for improving callus and shoot formation and regeneration of plants using sodium nitroprusside (SNP) in combination with Brassica napus BABYBOOM (BnBBM).

The invention provides a chemotherapy combined medicine and application thereof. The chemotherapy combined medicine comprises a first medicine and a second medicine, the first drug is a platinum chemotherapeutic drug or adriamycin, and the second drug is a zinc nitroprusside nano-drug; the zinc nitroprusside nano-drug is formed by coordination self-assembly of sodium nitroprusside and a zinc ion source compound. The zinc nitroprusside nano-drug has glutathione responsiveness and can release nitric oxide and zinc ions. In the platinum chemotherapy combined medicine, the cis-platinum and the ZnNO play a synergistic interaction role.

The invention provides a method for improving the developmental capacity of a frozen pig IVF (in vitro fertilization) embryo, which comprises the following steps: in the production of a pig in vitro embryo, before the pig embryo obtained by in vitro fertilization is cryopreserved, carrying out combined treatment by using fenofibric acid, sodium nitroprusside, FTY720-P and dopamine, then carrying out vitrification and unfreezing on the embryo, and during the freezing and unfreezing treatment, carrying out freeze-drying on the embryo to obtain the frozen pig IVF embryo. Z-VAD-FMK and TGF-beta are added into an embryo pretreatment solution, a vitrification freezing solution, an unfreezing solution and an embryo culture solution, so that the in-vitro developmental ability of the porcine embryo after vitrification freezing is improved. Experiments show that the embryo survival rate and the hatching rate of a group in which fenofibric acid, sodium nitroprusside, FTY720-P, dopamine, Z-VAD-FMK and TGF-beta are jointly added are obviously higher than those of a group in which fenofibric acid, sodium nitroprusside, FTY720-P, dopamine, Z-VAD-FMK and TGF-beta are added and a control group. The invention provides an effective, safe and feasible strategy for improving the pig in-vitro embryo production efficiency and embryo quality, and has great popularization value.

This invention belongs to the field of pharmaceutical synthesis technology and discloses a method for preparing sodium nitroprusside raw material. The method employs weak acid catalysis and significantly reduces the generation of toxic substances such as cyanide and nitrogen dioxide by controlling the material dropping rate and pH. The reaction is mild, the process is safe and controllable, and it facilitates the industrial production of sodium nitroprusside raw material. In some examples of the preparation method, silica gel filtration is used in the post-processing to reduce the residual risk of impurities such as sodium ferrocyanide, ferrous ferrocyanide, and ferric ferric ferrocyanide. The purity of the raw material is ≥99.9%, far exceeding the requirements of pharmacopoeias of various countries.

The invention discloses a method for establishing a fenugreek efficient regeneration system, and belongs to the technical field of medicinal plant tissue culture. The establishment method comprises the following steps: (1) inoculating a fenugreek explant into a primary induction culture medium containing 5-azacytidine, spermidine and fluridone, and carrying out induction culture to generate an adventitious bud; (2) dividing the adventitious buds, and then transferring the adventitious buds into a proliferation culture medium containing trehalose, paclobutrazol and uridine for culture to realize amplification of the adventitious buds; and (3) transferring the stem segments obtained by multiplication culture into a rooting culture medium containing sodium nitroprusside and jasmonic acid methyl ester for culture, and inducing rooting. According to the established efficient sterile rapid propagation system for fenugreek, the adventitious bud differentiation rate reaches 91.3%, the propagation coefficient is 10.8, the rooting rate reaches 97.5%, the propagation speed is high, high-quality seedlings can be produced on a large scale, and meanwhile the yield and quality are improved.

This invention discloses a method for improving the germination rate of *Hippophae rhamnoides* seeds. The method involves first soaking the *Hippophae rhamnoides* seeds in an ethephon solution, and then soaking the ethephon-treated seeds in a sodium nitroprusside solution. This solution solves the problem of low germination rate of *Hippophae rhamnoides* seeds in existing technologies.

The invention provides a sodium nitroprusside / prussian blue / manganese composite nano-enzyme and a preparation method and an antibacterial application thereof, and belongs to the technical field of nano-materials. The preparation method comprises the following steps: stirring and mixing polyvinylpyrrolidone, potassium ferricyanide and sodium nitroprusside in an acid solution at room temperature to prepare a prussian blue precursor solution; then, manganese acetate is dropwise added into the Prussian blue precursor solution, and sodium nitroprusside / Prussian blue / manganese composite nano-enzyme powder is obtained after high-pressure reaction, centrifugation and freeze drying; the sodium nitroprusside / prussian blue / manganese composite nano-enzyme prepared by the invention has a synergistic antibacterial mechanism of enzyme catalysis, photo-thermal / ultrasonic dynamic therapy and NO release, NO release is doubly regulated and controlled by laser or ultrasound, the photo-thermal effect and the sonodynamic effect are combined to destroy the structure and metabolism of bacteria, the composite nano-enzyme not only has an efficient killing effect on staphylococcus aureus, but also has a good antibacterial effect on the staphylococcus aureus. Bacterial biofilms can be removed, the problem of drug resistance mediated by the biofilms is effectively solved, and a new thought is provided for the antibacterial field.