30 results about "Sodium nitroprusside" patented technology

The present invention discloses a preparation method and production system for sodium nitroprusside. The preparation process comprises the following steps: first, reacting ferrocyanide with nitric acid to obtain a hydrogen nitroprusside aqueous solution; adding alcohol to the concentrated hydrogen nitroprusside aqueous solution to remove inorganic salts generated by the reaction, thereby obtaining a hydrogen nitroprusside alcohol solution; then, adding trisodium citrate to the hydrogen nitroprusside alcohol solution to react to obtain sodium nitroprusside and disodium citrate; separating and removing the citrate to obtain a sodium nitroprusside alcohol solution; and finally, concentrating, crystallizing, separating, and drying the sodium nitroprusside alcohol solution to obtain a sodium nitroprusside product. The present invention also provides a production system based on the preparation method. The production method of the present invention is simple to operate, operates under mild conditions, has a short production cycle, effectively prevents product oxidation, and has high purity. The obtained product is suitable for industrial production, and the production system requires less equipment and has low equipment requirements.

The invention discloses an MT (melatonin) and SNP (single nucleotide polymorphism) compound preservative as well as preparation and application thereof, and relates to the field of compound preservative films. A nitric oxide donor sodium nitroprusside (SNP); tween-80 (Tween-80) is added into auxiliary components to serve as an emulsifying agent, MT and SNP are dissolved in a proper amount of water and evenly stirred, then Tween-80 is added, and the MT and SNP compound preservative is prepared, according to the MT and SNP compound preservative and preparation and application thereof, MT and SNP are natural or quasi-natural components, compared with chemical preservatives, the MT and SNP have smaller potential harm to the environment and the human body, MT and SNP are used in a compound mode, and therefore the MT and SNP compound preservative has the beneficial effects that the MT and the SNP are more environmentally friendly and more environmentally friendly. MT and SNP cooperate with each other to improve the fresh-keeping effect, the antioxidant effect of MT can reduce oxidative damage in the fruits, SNP promotes endogenous NO generation, can regulate signal transduction in the fruits and further inhibits respiration and ethylene synthesis of the fruits, maturing and aging of the fruits are delayed from different angles through cooperation of MT and SNP, and the fresh-keeping effect of the fruits is improved. Meanwhile, the MT and the NO can cooperatively regulate and control signal transduction in the plant body, and the action time of the NO in the fruit body is prolonged.

The invention belongs to the technical field of medicine synthesis, and discloses a preparation method of a sodium nitroprusside raw material medicine. According to the method, weak acid is adopted for catalysis, the effect of remarkably reducing generation of toxic substances such as cyanide and nitrogen dioxide is achieved by controlling the material dripping speed and the dripping pH, the reaction is mild, the technological operation is safe and controllable, and industrial production of the sodium nitroprusside raw material medicine is facilitated. According to the preparation method of some examples, silica gel filtration is used for post-treatment, the residual risk of impurities such as sodium ferricyanide, ferric ferrocyanide and ferric ferricyanide is reduced, and the purity of the raw material medicine is larger than or equal to 99.9% and is far superior to the requirements of pharmacopoeia of various countries.

The invention discloses an underground propagule composite excitant for ecological restoration and a preparation method thereof, and relates to the technical field of excitant preparation. The fertilizer is prepared from the following raw materials in parts by weight: 0.2 to 0.3 part of gibberellin, 0.2 to 0.5 part of 6-benayl aminopurine, 0.2 to 0.3 part of indolebutyric acid, 0.5 to 0.7 part of sodium nitroprusside, 0.4 to 0.5 part of hydrogen peroxide, 1 to 2 parts of jasmonic acid sustained-release microspheres, 5 to 7 parts of compound amino acid, 1 to 3 parts of compound microbial agent sustained-release microspheres, 2 to 3 parts of seaweed extract, 10 to 13 parts of humic acid, 2 to 3 parts of monopotassium phosphate, 60 to 74 parts of ethanol and 1000 parts of water. The underground propagule composite excitant provided by the invention is beneficial to promoting activation and germination of underground propagules, enhancing plant growth and stress resistance, improving soil micro-ecological functions, forming stable diversity communities and facilitating ecological restoration.

The present invention relates to a sodium nitroprusside pharmaceutical composition and a method for determining cyanide therein. Specifically, on the one hand, it relates to a method for determining the cyanide content in a sodium nitroprusside pharmaceutical composition using gas chromatography, the method comprising the following steps: providing a gas chromatograph, a capillary chromatographic column and a derivatization reaction flask; preparing a derivatization reagent and a test sample solution, etc., placing them outside the outer bottle of the derivatization reaction flask, and adding the derivatization reagent into the inner bottle of the derivatization reaction flask, covering and sealing; using the headspace injection method, performing gas chromatography testing with high-purity nitrogen as the carrier gas, performing analysis using a programmed temperature rise, recording the chromatograms of the reference solution and the test sample solution, and calculating the cyanide content in the test sample by peak area according to the external standard method. It also relates to the derivatization reaction flask used in the above method, and a method for preparing the sodium nitroprusside pharmaceutical composition. The method of the present invention exhibits the effects of one or more aspects as described in the specification, such as high test efficiency and accurate test results.

The present invention provides a preparation method for a nano-drug composite dynamic cross-linked hydrogel with curcumin / nitric oxide / photothermal synergistic antibacterial effect. The present invention uses copper salt to chelate curcumin to form first nanoparticles, and then uses polydopamine to coat the first nanoparticles to form nano-drug P Cur Sodium nitroprusside and sodium phytate were used as raw materials and nano-coprecipitation method was used to prepare second nanoparticles; polydopamine was then used to coat the second nanoparticles to prepare a core-shell structure nano drug P NO ; The nano drug P cur and P NO The nanocomposite hydrogel is mixed evenly with carboxymethyl chitosan and oxidized hyaluronic acid to form a nanocomposite dynamically cross-linked hydrogel. In addition to its significant antibacterial properties, the nanocomposite hydrogel of the present invention also has significant advantages such as high antibacterial efficacy, good biosafety, convenient preparation, and low cost. The present invention also combines drug, photothermal, and gas therapy in a single device, facilitating synergistic and precise bacterial elimination.

The invention discloses an improved test method for detecting a dairy cow ketone body and application, and relates to the technical field of dairy product detection, and the method comprises the following steps: a lactalbumin detection step: pretreating a dairy cow sample, mixing the pretreated dairy cow sample with sodium nitroprusside-sodium carbonate composite powder, ammonium sulfate and anhydrous sodium carbonate according to a preset ratio, and determining the lactalbumin content through reaction color development; a urinary ketone detection step: pretreating a dairy cow urine sample, mixing the pretreated dairy cow urine sample with sodium nitroprusside-sodium carbonate composite powder, ammonium sulfate and anhydrous sodium carbonate in proportion, and judging the content of urinary ketone by prolonging the reaction time and adjusting the dosage of ammonium sulfate; the kit covers rapid screening of dairy farms, clinical stage diagnosis of veterinarians and raw material quality control of dairy enterprises, and is compatible with sample and urine sample detection. Through an accurate reagent system and parameter optimization, high-sensitivity, low-cost and fast detection is realized, and a technical support is provided for ketosis prevention and control.

This invention belongs to the pharmaceutical field, specifically relating to a sodium nitroprusside injection and its preparation method. The sodium nitroprusside injection comprises the following raw materials in parts by weight: 1.5–3.5 parts sodium nitroprusside and 100 parts water for injection. This invention solves the problem of poor stability of sodium nitroprusside aqueous solutions by optimizing the formulation and preparation process without introducing other excipients. The sodium nitroprusside injection prepared by this invention is a sterile injectable preparation manufactured using modern aseptic filling technology combined with non-terminal sterilization. It not only has the advantages of direct and rapid action and high sterility assurance, but also has few adverse reactions and can be transported and stored at room temperature, thus improving the stability and safety of the product. This invention also provides its preparation method, which is simple, safe to use, and has stable quality.

The present application relates to the technical field of nanomaterial application, and provides a ruthenium-doped Prussian blue nanoscale enzyme and a preparation method thereof, wherein the ruthenium-doped Prussian blue nanoscale enzyme is obtained by reacting metal ruthenium with Prussian blue nanoparticles under normal temperature conditions. The present application also provides a nanoscale enzyme library containing the ruthenium-doped Prussian blue nanoscale enzyme and a construction method thereof. The present application also provides SNP-PB nanoscale enzyme obtained by loading sodium nitroprusside into the ruthenium-doped Prussian blue nanoscale enzyme. The ruthenium-doped Prussian blue nanoscale enzyme and the SNP-PB nanoscale enzyme both have good antibacterial and anti-inflammatory properties, and can better solve the problem of biofilm resistance after being endowed with the ability to release NO gas, thereby better exerting antioxidant stress and fighting periodontal bacterial infection, and providing a new treatment method for periodontal disease.

The invention discloses a method for preparing a feather-shaped Prussian blue-based material NiFe-PBA by adopting a hydrothermal method. According to the method, a hydrothermal method is used, a sodium nitroprusside (Na3Fe [(CN) 5NO]) solution is used as a solvent, and then clean foamed nickel is added. Reacting for 4 hours at the high temperature of 160 DEG C; the feather-shaped NiFe-PBA catalyst with different sizes is prepared by regulating and controlling the concentration of a sodium nitroprusside solution in an experiment, and the catalyst has excellent urea oxidation reaction performance (UOR). In an experiment, the synthesized feather-shaped NiFe-PBA-X (X = 0.005, 0.01, 0.05, 0.1, mol dm <-3 >) catalyst is synthesized and then is washed with water and ethanol respectively, finally, the catalyst is dried in a drying oven at 40 DEG C for 6 hours, and the catalyst is taken out and used for a performance test of electro-oxidation urea.

The invention discloses a method for measuring the content of cyanide in sodium nitroprusside by potentiometric titration, which comprises the following steps: taking a cyanide ion selective electrode as an indicator electrode, adding disodium ethylene diamine tetraacetate into a solution to be measured, and measuring the content of cyanide in sodium nitroprusside under an alkaline condition. The method is easy to operate, high in safety, high in sensitivity, good in precision and high in accuracy, and the content of cyanide in sodium nitroprusside and related preparations of sodium nitroprusside can be rapidly and accurately measured.

The present invention discloses a method for promoting the growth of submerged plants under the stress of flocculants, and the steps are as follows: Select well-growing submerged plant seedlings. After pruning and cleaning, pre-culture them in 5-15% Hoagland nutrient solution, select seedlings with consistent growth, and obtain the seedlings to be planted after cleaning; Under light-shielded conditions, prepare a sodium nitroprusside pretreatment solution with a concentration of 100-400 μM using a CaCl2 solution; Under constant temperature and light-shielded conditions, completely immerse the seedlings to be planted in the pretreatment solution and pretreat for 3-5 hours, then wash them clean with deionized water and set aside; Transplant the pretreated seedlings into the water body containing flocculants. This method addresses the problem of inhibited growth of submerged plants caused by flocculant pollution in water bodies, improves the tolerance of plants to the toxicity of flocculants, promotes the growth of plants in a flocculant environment, is applicable to the restoration and reconstruction of submerged plants in water bodies with different flocculant accumulation amounts, and has the advantages of simple operation, economic ecology, and remarkable effects.

The present invention provides a method for preparing a high-purity sodium nitroprusside raw material. Take the crude sodium nitroprusside and dissolve it in water at 50-60 °C, drop in anhydrous fatty alcohol, cool down to 20-25 °C, stir, add the supramolecular polymer TPAP-Mb@tQ[14], carry out ultrasonic oscillation, let it stand, perform high-speed centrifugal separation, filter, and collect the filtrate; carry out reduced-pressure distillation until no distillate is distilled out; add water, stir at 40-60 °C, cool to 10 °C and stir, filter, collect the filter cake, and dry it to obtain the first refined product of sodium nitroprusside. The provided preparation method has simple operation, great feasibility in industrial operation, less three-waste discharge, is green, environmentally friendly and sustainable, meets the requirements of industrial safe production, meets the requirements of industrial cost reduction and environmental protection, is conducive to large-scale industrial production, the purity of the prepared sodium nitroprusside is above 99%, up to 100% at most, and the yield is also above 85%, showing significant improvement compared with the prior art process and being more suitable for industrialization.

The invention relates to the technical field of urea detection, in particular to a urea residue detection kit and a detection method thereof. The provided urea residue detection kit comprises the following components: a phosphate-EDTA buffer solution with a concentration of 20-22 mmol / L; an alkaline sodium hypochlorite solution with a concentration of 30-32 mmol / L; a color developing solution containing 0.8 to 1.0 mmol / L of phenol-sodium nitroprusside; the invention discloses an elisa plate coupled with urease. According to the kit, urea can be hydrolyzed by urease to generate ammonium ions and carbon dioxide, chloramine generated by oxidation of sodium hypochlorite and phenol are catalyzed by sodium nitroferricyanide under an alkaline condition to generate blue indophenol blue, a product has a characteristic absorption peak at 630 nm, and the content of urea is determined through the change of a light absorption value. The detection kit is high in sensitivity, high in detection speed, clear in result judgment, good in durability and beneficial to wide application.

The utility model relates to the technical field of chemical production, in particular to a stirring mechanism for small-scale production of sodium nitroprusside, which comprises a stirring barrel, a top cover is fixedly mounted on the upper end surface of the stirring barrel, a lifting device is arranged on the upper end surface of the top cover and comprises a lifting adjusting screw rod, and the lifting adjusting screw rod is rotatably sleeved on the right side of the upper end surface of the top cover; the outer side of the lifting adjusting screw is in threaded connection with a lifting support sleeve, the lower end of the lifting support sleeve is fixedly connected with a stirring motor, the output end of the stirring motor is fixedly connected with a stirring rod, the lower end of the stirring rod is connected with a powder stirring device, the powder stirring device comprises an elastic telescopic spline rod, and the elastic telescopic spline rod elastically sleeves the bottom of the stirring rod; the stirring mechanism has the beneficial effects that the powder stirring device is arranged, so that solid powder settled at the bottom can be properly stirred in a solid-liquid mixing reaction, the solid powder is sufficiently diffused in a solution, the reaction rate is increased, and the reaction is sufficient.

This invention provides a sodium nitroprusside / Prussian blue / manganese composite nanozyme, its preparation method, and its application in antibacterial applications. Belonging to the field of nanomaterials technology, the preparation method involves: first, polyvinylpyrrolidone, potassium ferricyanide, and sodium nitroprusside are mixed in an acidic solution at room temperature to prepare a Prussian blue precursor solution; then, manganese acetate is added dropwise to the Prussian blue precursor solution; after high-pressure reaction, centrifugation, and freeze-drying, sodium nitroprusside / Prussian blue / manganese composite nanozyme powder is obtained. The sodium nitroprusside / Prussian blue / manganese composite nanozyme prepared by this invention possesses a synergistic antibacterial mechanism of "enzyme catalysis + photothermal / ultrasound-based kinetic therapy + NO release." Through dual regulation of NO release via laser or ultrasound, combined with photothermal and acoustic-dynamic effects, it disrupts bacterial structure and metabolism, exhibiting not only highly effective killing of Staphylococcus aureus but also clearing bacterial biofilms, effectively solving the problem of biofilm-mediated drug resistance and providing a new approach in the field of antibacterial research.

The present invention relates to the field of plant breeding and in particular to the regeneration of plants from cells and other tissues. More particularly, the invention provides methods and means for improving callus and shoot formation and regeneration of plants using sodium nitroprusside (SNP) in combination with Brassica napus BABYBOOM (BnBBM).

The invention provides a chemotherapy combined medicine and application thereof. The chemotherapy combined medicine comprises a first medicine and a second medicine, the first drug is a platinum chemotherapeutic drug or adriamycin, and the second drug is a zinc nitroprusside nano-drug; the zinc nitroprusside nano-drug is formed by coordination self-assembly of sodium nitroprusside and a zinc ion source compound. The zinc nitroprusside nano-drug has glutathione responsiveness and can release nitric oxide and zinc ions. In the platinum chemotherapy combined medicine, the cis-platinum and the ZnNO play a synergistic interaction role.

The invention relates to the technical field of cut flower post-harvest physiological regulation and control, in particular to high-openness cut babysbreath anti-aging pre-treatment liquid based on moisture-energy-ethylene coordinated regulation and a negative pressure permeation treatment method of the high-openness cut babysbreath anti-aging pre-treatment liquid. The pretreatment liquid is prepared from the following components in percentage by weight / volume: 20 to 30 g / L of trehalose, 300 to 400 mg / L of 8-hydroxyquinoline sulfate, 150 to 200 mg / L of ammonium citrate, 5 to 15 mg / L of nano-silver sol, 15 to 25 mg / L of gibberellin, 25 to 50 mg / L of salicylic acid, 5 to 20 g / L of chitosan quaternary ammonium salt, 10 to 20 mg / L of sodium nitroprusside and 0.5 to 1.5 g / L of calcium nitrate. And 1.0 to 5.0 mL / L of Tween-20 in percentage by volume. According to the method disclosed by the invention, particularly aiming at the pretreatment of the cut babysbreath with high openness, multiple and multi-dimensional protective nets are formed through physical (negative pressure forced penetration), chemical (8-HQS) synergistic penetration system (dual-channel water absorption technology), dynamic moisture regulation and control technology (chitosan quaternary ammonium salt and trehalose) and ethylene-NO-hormone synergistic multi-dimensional blocking senescence signals; the bottleneck of high-openness babysbreath preservation is broken through, and the service life of high-openness babysbreath commodities is prolonged from 5 days to 12 days or above.

The invention belongs to the field of plant protection, and provides a poplar leaf rust control composition and a poplar leaf rust control method. The prevention and treatment composition for poplar leaf rust comprises 30-65 [mu] M of abscisic acid and 130-270 [mu] M of sodium nitroprusside. A prevention and control method for poplar leaf rust comprises the steps that an abscisic acid solution and a sodium nitroprusside solution are prepared, the concentration of the abscisic acid ranges from 30 mu M to 65 mu M, and the concentration of the sodium nitroprusside ranges from 150 mu M to 270 mu M; in the poplar young leaf period, the abscisic acid and sodium nitroprusside solution is sprayed to the back faces of poplar leaves; after spraying, covering the young leaves of the poplar with black plastic bags, and removing the black plastic bags after 24 hours, or selecting the spraying time at nightfall or at night. The composition for preventing and treating poplar leaf rust and the prevention and treatment method for poplar leaf rust can be sprayed on poplar young leaves, and the prevention and treatment problem of poplar leaf rust is solved.

The invention provides a method for improving the developmental capacity of a frozen pig IVF (in vitro fertilization) embryo, which comprises the following steps: in the production of a pig in vitro embryo, before the pig embryo obtained by in vitro fertilization is cryopreserved, carrying out combined treatment by using fenofibric acid, sodium nitroprusside, FTY720-P and dopamine, then carrying out vitrification and unfreezing on the embryo, and during the freezing and unfreezing treatment, carrying out freeze-drying on the embryo to obtain the frozen pig IVF embryo. Z-VAD-FMK and TGF-beta are added into an embryo pretreatment solution, a vitrification freezing solution, an unfreezing solution and an embryo culture solution, so that the in-vitro developmental ability of the porcine embryo after vitrification freezing is improved. Experiments show that the embryo survival rate and the hatching rate of a group in which fenofibric acid, sodium nitroprusside, FTY720-P, dopamine, Z-VAD-FMK and TGF-beta are jointly added are obviously higher than those of a group in which fenofibric acid, sodium nitroprusside, FTY720-P, dopamine, Z-VAD-FMK and TGF-beta are added and a control group. The invention provides an effective, safe and feasible strategy for improving the pig in-vitro embryo production efficiency and embryo quality, and has great popularization value.

The invention relates to an improved preparation method of high-purity sodium nitroprusside dihydrate, which comprises the following steps of: 1, reacting potassium ferrocyanide or sodium ferrocyanide with nitric acid in a solvent, then adding sodium carbonate or potassium carbonate for reaction, and then adding water-soluble bivalent copper salt or water-soluble bivalent zinc salt for reaction to obtain copper nitroprusside or zinc nitroprusside; 2, reacting copper nitroprusside or zinc nitroprusside with sodium bicarbonate in a solvent to obtain a sodium nitroprusside crude product, and purifying the sodium nitroprusside crude product to obtain sodium nitroprusside dihydrate; in the step 2, citric acid is added to adjust the pH value to 5-7 after reaction with sodium bicarbonate. The process product has the advantages of high yield, high purity, simple reaction and post-treatment conditions and strong operability, and is very suitable for process scale-up production.

The present invention discloses a step-by-step laser-stimulated multi-active oxygen species-releasing wound dressing, as well as its preparation method and application. The wound dressing is prepared by chemically grafting protoporphyrin and upconversion nanoparticles onto a carboxylated cellulose nanofiber matrix to produce smart nanofibers responsive to white light and 980nm lasers. The nanofibers are then reacted with a thermosensitive reagent through hydrogen bonding and electrostatic interactions to form fiber balls. The spherical fibers are then simultaneously loaded with indocyanine green, sodium nitroprusside, and doxorubicin in the cavities of the balls to produce the step-by-step laser-stimulated multi-active oxygen species-releasing wound dressing. The wound dressing can respond gradually to three lasers with different penetration depths and release different levels of reactive oxygen species.

The invention relates to the technical field of preservation of picked fresh cut flowers, and discloses a delphinium grandiflorum cut flower pre-treatment liquid based on synergistic interaction of natural components and a preparation method of the delphinium grandiflorum cut flower pre-treatment liquid. The pretreatment liquid is prepared from the following components in percentage by mass / volume: 50 to 150 mg / L of 8-hydroxyquinoline sulfate, 20 to 30 mg / L of sodium dichloroisocyanurate, 100 to 250 mg / L of citric acid, 0.8 to 1.2 mg / L of 1-MCP, 50 to 100 [mu] mol / L of sodium nitroprusside, 50 to 100 mg / L of ascorbic acid, 40 to 80 mg / L of aluminum sulfate, 30 to 150 mg / L of proline and 0.01 to 0.5 percent of plant extract. According to the present invention, the sterilization, the ethylene resistance, the nutrition supplement, the physical support and the oxidation resistance are integrated, such that the limitation of the single component is broken through, the high toxicity of STS and AgNO3 in the traditional formula is solved, the environmental toxicity is reduced, the vase life is prolonged to 23-28 days, and the petal falling rate is reduced by 70%. Meanwhile, step-by-step dissolution and ultrasonic extraction are adopted to ensure the activity of the components, and a simplified pretreatment method is adopted, so that the method is suitable for fresh cut flower manufacturers, logistics enterprises and terminal retailers.

This invention belongs to the field of pharmaceutical synthesis technology and discloses a method for preparing sodium nitroprusside raw material. The method employs weak acid catalysis and significantly reduces the generation of toxic substances such as cyanide and nitrogen dioxide by controlling the material dropping rate and pH. The reaction is mild, the process is safe and controllable, and it facilitates the industrial production of sodium nitroprusside raw material. In some examples of the preparation method, silica gel filtration is used in the post-processing to reduce the residual risk of impurities such as sodium ferrocyanide, ferrous ferrocyanide, and ferric ferric ferrocyanide. The purity of the raw material is ≥99.9%, far exceeding the requirements of pharmacopoeias of various countries.

The invention discloses a method for establishing a fenugreek efficient regeneration system, and belongs to the technical field of medicinal plant tissue culture. The establishment method comprises the following steps: (1) inoculating a fenugreek explant into a primary induction culture medium containing 5-azacytidine, spermidine and fluridone, and carrying out induction culture to generate an adventitious bud; (2) dividing the adventitious buds, and then transferring the adventitious buds into a proliferation culture medium containing trehalose, paclobutrazol and uridine for culture to realize amplification of the adventitious buds; and (3) transferring the stem segments obtained by multiplication culture into a rooting culture medium containing sodium nitroprusside and jasmonic acid methyl ester for culture, and inducing rooting. According to the established efficient sterile rapid propagation system for fenugreek, the adventitious bud differentiation rate reaches 91.3%, the propagation coefficient is 10.8, the rooting rate reaches 97.5%, the propagation speed is high, high-quality seedlings can be produced on a large scale, and meanwhile the yield and quality are improved.

This invention discloses a method for improving the germination rate of *Hippophae rhamnoides* seeds. The method involves first soaking the *Hippophae rhamnoides* seeds in an ethephon solution, and then soaking the ethephon-treated seeds in a sodium nitroprusside solution. This solution solves the problem of low germination rate of *Hippophae rhamnoides* seeds in existing technologies.

The invention provides a sodium nitroprusside / prussian blue / manganese composite nano-enzyme and a preparation method and an antibacterial application thereof, and belongs to the technical field of nano-materials. The preparation method comprises the following steps: stirring and mixing polyvinylpyrrolidone, potassium ferricyanide and sodium nitroprusside in an acid solution at room temperature to prepare a prussian blue precursor solution; then, manganese acetate is dropwise added into the Prussian blue precursor solution, and sodium nitroprusside / Prussian blue / manganese composite nano-enzyme powder is obtained after high-pressure reaction, centrifugation and freeze drying; the sodium nitroprusside / prussian blue / manganese composite nano-enzyme prepared by the invention has a synergistic antibacterial mechanism of enzyme catalysis, photo-thermal / ultrasonic dynamic therapy and NO release, NO release is doubly regulated and controlled by laser or ultrasound, the photo-thermal effect and the sonodynamic effect are combined to destroy the structure and metabolism of bacteria, the composite nano-enzyme not only has an efficient killing effect on staphylococcus aureus, but also has a good antibacterial effect on the staphylococcus aureus. Bacterial biofilms can be removed, the problem of drug resistance mediated by the biofilms is effectively solved, and a new thought is provided for the antibacterial field.

The invention discloses a method for relieving waterlogging disasters in a cotton flowering and boll-forming period through cooperation of shading and chemical regulation and control. The method comprises the steps that a sunshade net with the light transmittance of 35%-55% is erected within 12-24 hours after the cotton field is waterlogged, and the operation lasts for 5-6 days after water recession; and spraying a compound solution of sodium nitroprusside and melatonin within 2 hours after the shading is relieved. The waterlogging disaster in the cotton flowering and boll-setting period is relieved through the cooperation of shading and chemical regulation, the yield loss caused by waterlogging-high temperature cooperative stress can be reduced by 35% or above, salt-waterlogging-high temperature composite stress is relieved, and the yield loss is reduced by 40% or above.

The invention relates to the technical field of cut flower postharvest treatment, and discloses a pre-treatment solution for promoting flower forcing quality of low-openness babysbreath cut flowers and a preparation method and application thereof. The pre-treatment liquid is prepared from the following components in percentage by weight / volume: 20 to 60 g / L of cane sugar, 100 to 250 mg / L of 8-hydroxyquinoline citrate, 50 to 200 mg / L of citric acid, 0.5 to 1.5 g / L of calcium nitrate, 0.3 to 0.8 g / L of aluminum sulfate and 50 to 100 [mu] mol / L of sodium nitroprusside; and 0.3 to 1.0 mL / L of tween-20 by volume percent. The method further comprises the following components in percentage by weight / volume: 10-30 mg / L of gibberellin and 50-100 mg / L of salicylic acid, particularly for the post-harvest flower pretreatment stage of the babysbreath cut flowers with low openness, a whole-course shape protection scheme from harvesting to flower forcing is provided for the cut flowers through triple synergy of energy supply, antibiosis and anticorrosion and physiological regulation, and the flower forcing flowering rate and the ornamental quality are remarkably improved. The invention further provides an efficient and stable preparation process of the pretreatment liquid, the stability and permeability of the components are ensured, and the pretreatment liquid is suitable for cut flower production, logistics and retail end pretreatment links and has remarkable economic benefits.