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Method for rapidly detecting nutrient content of plant by extracting

A technology for nutrient components and detection methods, which is applied in the field of rapid extraction and detection methods for plant nutrient components, can solve problems such as shortening detection time, inability to perform quantitative detection and analysis at the same time, and achieve the effects of cost saving and rapid analysis.

Inactive Publication Date: 2010-10-20
SOYBEAN TECH DEV RES CENT HEILONGJIANG PROV
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Problems solved by technology

[0008] In order to solve the problem that the multiple target components contained in the same seed in the same generation cannot be quantitatively detected and analyzed at the same time during the individual selection process of soybean functional nutrient component gene aggregation breeding hybrid offspring, the purpose of the present invention is to provide a An extraction and detection method to achieve "one-time sampling, multiple functional nutritional components can be extracted and detected simultaneously", thereby speeding up the selection process of breeding offspring and cultivating new high value-added soybean varieties; at the same time, in order to shorten the detection time and reduce manpower and material resources , Financial investment, reduce environmental pollution caused by detection waste

Method used

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  • Method for rapidly detecting nutrient content of plant by extracting
  • Method for rapidly detecting nutrient content of plant by extracting
  • Method for rapidly detecting nutrient content of plant by extracting

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Embodiment 1

[0054] Extract functional nutrients

[0055] like figure 1 As shown, the mature soybean hybrid seeds to be detected are cut off with a paper blade one by one on the opposite side of the hilum (subject to the fact that the germ, radicle, and hypocotyl are not damaged at all) (detection of immature soybean cotyledon In the case of immature soybean cotyledons, freeze-dry the immature soybean cotyledon for 24 hours by a conventional freeze dryer, grind and pulverize into a powder) 10 mg is added to a 1.5 ml eppendorf centrifuge tube, and at the same time, β-apocarotene aldehyde containing 0.25 μg / ml is added (β-Apo-8'-carotenal (trans)) and 3 μg / ml tocol (Tocol) internal standard ice-cold n-hexane reagent 500 μl, shake the oscillator for 10-20 seconds, ultrasonic at 4 ° C for 8-10 minutes , then refrigerated in the dark for 15-30 minutes, centrifuged at 13,000 rpm for 10 minutes, took 300-400 μl of the supernatant for HPLC analysis of carotenoids such as vitamin E and lutein; too...

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Abstract

The invention provides a method for rapidly detecting the nutrient content of a plant, in particular the nutrient content of a soybean polygene polymerization breeding filial generation. The method comprises the following steps of: extracting primary samples of substances, such as a water-soluble substance, namely, isoflavone and fat-soluble substances, namely, vitamin E, xanthophyll, carotene and the like at the same time; and performing a high performance liquid chromatography (HPLC) two-step analysis method to realize synchronous analysis and detection of various functional nutrients through primary samples. Therefore, the method has the advantages of accelerating the selection process of breeding generations, cultivating a new soybean variety with a high added value, shortening detection time, reducing the investments on manpower, material resources and financial resources and reducing the pollution of wastes to the environment.

Description

technical field [0001] The present invention relates to a method for quickly extracting and detecting plant nutritional components, especially the content of functional nutritional components in soybeans, in particular to a method capable of synchronously and rapidly extracting functional nutritional components of hybrid offspring of soybean multi-gene polymerization breeding, and using HPLC to obtain It has a detection function, which can be used for qualitative and quantitative methods, so that its hybrid offspring can be quickly screened. Background technique [0002] Many plant seeds, especially soybean seeds are not only rich in high-quality vegetable protein and fatty acids, but also contain a variety of functional nutrients such as isoflavones and vitamin E. Especially in recent years, with the improvement of living standards, consumers' demand for functional nutrients such as soybean isoflavones and vitamin E has risen sharply. However, among the existing soybean va...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/36
Inventor 王绍东冯晓王晓云李文滨喜多村启介朱秀清李远明富校轶刘伟姜妍王浩刘燕李宏伟张平姚磊
Owner SOYBEAN TECH DEV RES CENT HEILONGJIANG PROV
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