Bacillus thuringiensis suspending agent for killing cotton spider mites, preparation and use

A technology of Bacillus chrysogenum and Bacillus thuringiensis, applied in acaricides, botanical equipment and methods, biochemical equipment and methods, etc., to achieve the effects of avoiding cross-resistance, preventing soil salinization, and expanding the insecticidal spectrum

Active Publication Date: 2009-07-15
ECOLOGY INST SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

See Liu Shiquan, Research Progress on High-potency Insecticides of Bacillus thuringiensis (Microbiology Bulletin, 2008, 35(7): 1091-1095), which has high-efficiency acaricidal activity against Tetranychus cotton, and is also effective against Lepidoptera, bismuth The strains with insecticidal activity for some pests and plant nematodes of Pteroptera and Coleoptera have not been reported yet

Method used

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  • Bacillus thuringiensis suspending agent for killing cotton spider mites, preparation and use
  • Bacillus thuringiensis suspending agent for killing cotton spider mites, preparation and use
  • Bacillus thuringiensis suspending agent for killing cotton spider mites, preparation and use

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Experimental program
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Effect test

Embodiment 1

[0042] Embodiment 1: Isolation and identification of bacterial strain BtR05

[0043] 1. The isolation steps of strain BtR05 are as follows:

[0044] (1) Collection of samples: the samples were collected from vegetable fields in Jinan, and the topsoil layer at a depth of 0-10 cm was taken with a shovel, packed in polyethylene bags, and brought back to the laboratory.

[0045] (2) Separation method: take 1g of soil sample in BPA medium (BPA medium: 5g of beef extract, 10g of peptone, 34g of sodium acetate, 1000mL of water, pH7.2~7.5) and shake fully, then place it in a shaker at 30°C Cultivate for 6 hours, take it out and place it at 75-80°C for heat treatment for 10 minutes, after a little rest, take 0.5mL in BP medium (beef extract 3g, peptone 5g, sodium chloride 5g, agar 15g, distilled water 1000mL, pH7.0~ 7.2) Spread evenly on the plate; culture at 30°C for 24 hours, select a colony with characteristics similar to Bacillus thuringiensis and inoculate it on the BP slant; cul...

Embodiment 2

[0057] Embodiment 2: Liquid fermentation of bacterial strain BtR05, said percentages are mass percentages.

[0058] Bacillus thuringiensis BtR05, with the preservation number CGMCCNO.2823, is isolated and screened from soil. The pathogenicity research on Tetranychus cotton shows that the strain has strong acaricidal ability, easy cultivation and no pollution.

[0059] The liquid fermentation of bacterial strain BtR05 is carried out in the following steps:

[0060] (1) Bacteria on a slant: using solid LB medium, Bacillus thuringiensis BtR05 was inoculated on the test tube medium, and cultured at 30° C. for 24 hours.

[0061] (2) Shake flask strains: use liquid LB medium, inoculate the test tube strains in the liquid LB medium, place on a shaker and culture at 30° C. for 18 hours with shaking.

[0062] (3) Liquid fermentation: use culture medium, 2% corn flour, 6% bean foil powder, 1% sucrose, 0.5% (NH 4 ) 2 SO 4 , 0.2% K 2 HPO 4 , 0.2% KH 2 PO 4 , 0.03% MgSO 4 ·7H 2 O...

Embodiment 3

[0063] Embodiment 3: preparation of BTD suspension concentrate, said percentages are all mass percentages, taking the amount of fermentation broth as the base.

[0064] Add 5% ammonium sulfate, 0.15% sodium benzoate, 0.1% fatty alcohol polyoxyethylene ether, 0.20% xanthan gum to the fermented liquid in embodiment 2. Note that when preparing the suspending agent, ammonium sulfate should be slowly and continuously stirred into the fermentation broth. After the ammonium sulfate is completely dissolved, slowly add sodium benzoate, fatty alcohol polyoxyethylene ether, and xanthan gum in sequence.

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Abstract

The invention relates to a suspending agent sterilizing two-spotted spider mite Bacillus thuringiensis, belonging to the field of biological pesticides. The suspending agent is prepared by mixing fermentation liquid of the Bacillus thuringiensis BtR05 with a surfactant, an antiseptic agent, a suspending auxiliary agent and a thickening agent, wherein, the suspending auxiliary agent is ammonium sulphate. The invention also provides preparation methods and uses of the suspending agent, the suspending agent prepared by the BtR05 bacterium and the fermentation liquid of the bacterium is quite poisonous against two-spotted spider mite, American white moth, diamond-back moth, chive maggot, grub, maggot galerucella and Meloidogyne incognita, which is an efficient and broad-spectrum biological pesticide.

Description

technical field [0001] The invention relates to a Bacillus thuringiensis (Bacillus thuringiensis) BtR05 strain with high-efficiency acaricidal activity against cotton spider mites and a preparation method and application of a suspension, belonging to the technical field of biological pesticides. Background technique [0002] Cotton spider mite, also known as cotton spider mite, belongs to Arachnida, Acaridae, and Tetranychidae pests. It occurs in various cotton areas. In addition to harming cotton, it also harms corn, sorghum, wheat, soybeans, etc., and has a wide range of hosts. Cotton spider mite mainly pricks and sucks juice on the back of cotton leaves, causing damage symptoms such as yellow spots, red leaves and fallen leaves on the leaves. It looks like a fire, commonly known as a fire dragon. In the year of the outbreak, it caused a large area of ​​production reduction or even no harvest. See the occurrence and control techniques of cotton spider mites (Jingxian Agri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01N25/02A01P5/00A01P7/02A01P7/04C12R1/07
Inventor 杨合同李纪顺扈进冬王贻莲赵晓燕魏艳丽陈凯朱姣张广志吴远征黄玉杰周红姿
Owner ECOLOGY INST SHANDONG ACAD OF SCI
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