Strain for preventing and treating greenhouse tomato continuous cropping common diseases and composite microbial agent thereof

A compound microbial agent and tomato technology, applied in the direction of microorganisms, biocides, and methods based on microorganisms, can solve problems such as human health and environmental safety hazards, pathogenic bacteria resistance, and chemical residues in the environment, etc., to achieve recovery and stability Bacterial flora balance, less drug resistance, good control effect

Active Publication Date: 2021-03-30
CHAMBROAD CHEM IND RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to solve the problem of repeated cropping of tomatoes, common strategies include chemical pesticides, soil improvement, disease-resistant varieties, grafting and crop rotation, etc., but the effects are not stable
In addition, the control

Method used

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  • Strain for preventing and treating greenhouse tomato continuous cropping common diseases and composite microbial agent thereof
  • Strain for preventing and treating greenhouse tomato continuous cropping common diseases and composite microbial agent thereof
  • Strain for preventing and treating greenhouse tomato continuous cropping common diseases and composite microbial agent thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The preparation of embodiment 1 composite microbial bacterial agent

[0043] The YJY19-01 strain and the YJY20-02 strain were subjected to fermentation and expansion culture respectively. The culture conditions were: the aeration ratio of sterile air was 1:1.0, the stirring speed was 250rpm, the culture temperature was 37°C, and the fermentation period was 30h. The spore rate is not less than 80%.

[0044] Wherein, the culture medium is corn flour soybean flour medium, and its composition and weight percentage are: soybean meal 6.86%, corn flour 5.5%, ammonium sulfate 0.04%, corn starch 0.46%, manganese sulfate 0.05%, calcium carbonate 0.3%, Corn steep liquor dry powder 0.17%, the rest is water, pH7.2.

[0045] A. After the expanded culture is completed, the bacterial liquid is mixed according to the effective live bacteria amount of 1:1 to obtain a liquid composite microbial agent against common pathogens of repeated cropping, and the effective live bacteria amount is...

Embodiment 2

[0048] The preparation of embodiment 2 composite microbial bacterial agents

[0049] The YJY19-01 strain and the YJY20-02 strain were subjected to fermentation and expansion culture respectively. The culture conditions were: the aeration ratio of sterile air was 1:1.5, the stirring speed was 200rpm, the culture temperature was 35°C, and the fermentation period was 33h. The spore rate is not less than 80%.

[0050] Wherein, the culture medium is corn flour and soybean flour medium, and its composition and weight percentage are: 6.5% of soybean meal, 5% of corn flour, 0.1% of ammonium sulfate, 0.55% of corn starch, 0.034% of manganese sulfate, 0.1% of calcium carbonate, Corn steep liquor dry powder 0.3%, the rest is water, pH7.0.

[0051] A. After the expanded culture is completed, the bacterial liquid is mixed according to the effective live bacteria amount of 1:1 to obtain a liquid composite microbial agent against common pathogens of repeated cropping, and the effective live...

Embodiment 3

[0054] The preparation of embodiment 3 composite microbial bacterial agents

[0055] The YJY19-01 strain and the YJY20-02 strain were subjected to fermentation and expansion culture respectively. The culture conditions were: the aeration ratio of sterile air was 1:2.0, the stirring speed was 300rpm, the culture temperature was 33°C, and the fermentation period was 35h. The spore rate is not less than 80%.

[0056] Wherein, the culture medium is corn flour soybean flour medium, and its composition and weight percentage are: 6% soybean meal, 4.5% corn flour, 0.08% ammonium sulfate, 0.8% corn starch, 0.04% manganese sulfate, 0.25% calcium carbonate, Corn steep liquor dry powder 0.45%, the rest is water, pH7.4.

[0057] A. After the expanded culture is completed, the bacterial liquid is mixed according to the effective live bacteria amount of 1:1 to obtain a liquid composite microbial agent against common pathogens of repeated cropping, and the effective live bacteria amount is ≥...

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Abstract

The invention relates to the technical field of agriculture and microorganisms, in particular to a strain for preventing and treating greenhouse tomato continuous cropping common diseases and a composite microbial agent thereof. According to the invention, a YJY20-02 strain and YJY19-01 are compounded to obtain the composite microbial agent. After the compound microbial agent is applied, the fieldplanting is easy, the drug resistance is not easy to generate, and the compound microbial agent has obvious bacteriostatic activity on tomato continuous cropping germs, and the bacteriostatic activity is higher than the effect of single use of strains. The strain not only solves the practical problems of unstable prevention and treatment and weak persistence of a single biocontrol strain, but also has a good prevention and treatment effect on common tomato continuous cropping germs, can quickly recover and stabilize flora balance in soil, is a biocontrol strain which is high in tomato continuous cropping common disease prevention and treatment efficiency and environment-friendly, and is wide in application prospect and wide in application prospect. The invention also lays a foundation fordeveloping and developing a biocontrol combined preparation for effectively controlling tomato continuous cropping disease.

Description

technical field [0001] The invention relates to the technical fields of agriculture and microbes, in particular to a bacterial strain and a composite microbial bacterial agent for preventing and treating common diseases of tomato repeated cropping in greenhouses. Background technique [0002] Tomatoes are listed as one of the 30 most productive crops in the world. It is rich in vitamins and sugars, rich in nutrition and convenient to eat, so it is widely loved by people. With the expansion of the tomato cultivation area in my country in recent years, the continuous cropping obstacles such as low yield, poor quality, and many diseases of greenhouse tomatoes caused by the difficulty of stubble inversion have seriously restricted the development of the tomato industry and the improvement of economic benefits. [0003] With the continuous development of the tomato industry, crop rotation is difficult to implement, and the obstacle of repeated cropping has become a key issue of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00A01G22/05A01G13/00A01G7/06C12R1/125
CPCC12N1/20A01N63/22A01G22/05A01G13/00A01G7/06
Inventor 郭南南蔡颖辉杨传伦张心青张萧萧蔡倩倩马娜娜冉新新杨丹丹
Owner CHAMBROAD CHEM IND RES INST CO LTD
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