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Preparation method of compound microbial bacterium agent capable of effectively inducing formation of agilawood

A compound microbial agent and the technology of compound microbial bacteria are applied in the field of compound microbial agent for efficiently inducing agarwood to form aroma, which can solve problems such as poor quality, low yield of agarwood and aroma, and failure to meet market demand, and achieve a high success rate. Effect

Inactive Publication Date: 2017-07-25
SHANGHAI XCT HEALTH FOOD
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AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the current problems of low yield, poor quality and inability to meet the market demand of agarwood, and to provide a compound microbial bacterial agent for efficiently inducing agarwood to form

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0012] Preparation of compound microbial bacterial agent:

[0013] (1) Collection of strains: the complex microbial flora collected from the fragrant parts of Akiras sinensis grown in different regions and in different landforms;

[0014] (2) Enrichment culture: inoculate the collected different microbial complex flora on the liquid enrichment medium at 150 rpm, 33°C, and cultivate for 10 days;

[0015] (3) Expansion culture: Inoculate the bacterial liquid in the enrichment medium into the basic medium at 150 rpm at 33°C according to the inoculation amount of 5%, and carry out expansion;

[0016] (4) Preparation of composite microbial agent: centrifuge the above bacterial solution at 10,000 rpm, collect the bacterial cells for 10 minutes, remove the supernatant, add a protective agent, freeze at -20°C for 24 hours, and dry in vacuum.

[0017] Fragrance Test of Compound Microbial Inoculant on Aroma Tree:

[0018] (1) Agarwood and Agarwood: Inoculate the compound microbial age...

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Abstract

The invention discloses a preparation method of a compound microbial bacterium agent capable of effectively inducing formation of agilawood. The compound microbial bacterium agent is characterized by being selected and prepared sequentially by the following technological processes: extracting a compound microbial community, conducting enrichment culture, conducting centrifuging and collecting, conducting freeze-drying in a vacuum environment, promoting formation of the agilawood and conducting comprehensive analysis on an impregnating capacity. The compound microbial community provided by the invention can effectively induce the generation of the agilawood from agilawood plants on inoculation sites; and in addition, problems of existing single-fungus inducing which is low in agilawood yield and poor in agilawood quality, fails to meet a market demand and the like can be successfully solved.

Description

technical field [0001] The invention relates to a compound microbial bacterial agent for efficiently inducing the formation of agarwood and belongs to the field of biotechnology. Background technique [0002] Agarwood, also known as agarwood, is that the Agarwood plant encounters external force invasion or is infected by a certain virus or bacteria during its growth, so that the Agarwood plant itself produces an antibody to resist external aggression, and the antibody condenses on the Agarwood. Agarwood resin is a specific product formed in the xylem of plants. Agarwood plants cannot produce agarwood in healthy conditions. Even after the Agarwood plants are injured or invaded, they usually have to accumulate for several years to form Agarwood, and the output rate is very low, which is far from meeting the current market demand. People have taken various methods for making incense artificially for this reason, to improve the success rate of knotting incense and the output o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00A01G7/06
CPCA01G7/06C12N1/00
Inventor 朱恩灿杨丽陈金艳
Owner SHANGHAI XCT HEALTH FOOD
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