Bacillus composition for control of panax ginseng and American ginseng black spot disease and botrytis cinerea and use thereof

A technology of spores and compositions, which is applied in the field of spores compositions for the prevention and treatment of ginseng and American ginseng black spot and gray mold, can solve the problems of poor effect, survival, and large variation of strains, and achieve the prevention of no pesticide residues , Strong adaptability and strong adsorption capacity

Inactive Publication Date: 2017-10-13
HEILONGJIANG UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are currently Bacillus subtilis preparations on the market, because the strains come from non-ginseng production areas, the strains vary greatly, and the single strains used are usually difficult to survive on the leaves for a long time, and the effect is not good in practical applications. Bacteria is a good way

Method used

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  • Bacillus composition for control of panax ginseng and American ginseng black spot disease and botrytis cinerea and use thereof
  • Bacillus composition for control of panax ginseng and American ginseng black spot disease and botrytis cinerea and use thereof
  • Bacillus composition for control of panax ginseng and American ginseng black spot disease and botrytis cinerea and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Isolation, cultivation and identification test of ginseng dominant spores

[0041] 1. Obtain the material of ginseng dominant spore species

[0042] In August 2015, ginseng leaves were collected at the ginseng planting bases in Qing'an and Heihe, Heilongjiang Province, and multiple plants and leaves were selected.

[0043] 2. Bacillus isolation method

[0044] Cut ginseng leaves into 1-2cm pieces with sterile scissors, put them into conical flasks filled with 50mL sterile normal saline, vibrate at 120rpm for 1h (so that the bacteria fall off the leaves and disperse evenly in the normal saline), place in Incubate in a 63°C incubator for 4 hours (maintain at 63°C for 4 hours to kill various non-spores, molds, and pathogenic fungi, but the spores basically do not die), as samples to be separated.

[0045] Prepare sterilized CPDA medium to make a culture plate. Draw 0.5 mL of the above-mentioned heat-treated sample and evenly spread it on the CPDA medium cultur...

Embodiment 2

[0054] Example 2 strain screening test

[0055] 1. Materials

[0056] 87 strains of Bacillus subtilis, 60 strains of B. tequilensis and 62 strains of B. velezensis were isolated by the applicant from ginseng leaves. The pathogens of ginseng black spot and gray mold were provided by Shenyang Agricultural University.

[0057] 2. Method

[0058] The pathogenic bacteria of black spot and gray mold were purely cultured respectively. The culture conditions were to use CPDA agar medium plate and place it in a constant temperature incubator at 25°C for 3-4 days. The spores were used as the inoculated samples for the antagonistic experiments.

[0059] Bacillus subtilis, B. tequilensis, and B. velezensis were purely cultured respectively. The culture conditions were to use CPDA agar medium plates and place them in a constant temperature incubator at 25°C for 2 days to form visible to the naked eye. Small colonies were then inoculated into conical flasks equipped with liquid CPDA med...

Embodiment 3

[0067] Embodiment 3 field experiment

[0068] 1. Materials

[0069] According to the theory of biological invasion, the more complex the population of the community, the less likely it is for alien species to invade. Alien species are easy to invade a single population, and the single strain used is usually difficult to survive on the leaves for a long time, and the effect is not good in practical application. The compound bacterial agent is a good method. In order to ensure the stable colonization of spores, according to the ratio of the dominant bacteria in the natural state, the ratio of the above three antagonistic bacteria is: Bacillus subtilis: Bacillus tequila: Bacillus Velez = 1.5: 1: 1 composite bacteria agent for field trials.

[0070] The chemical fungicide is 80% mancozeb wettable powder; 50% cyprodinil water dispersible granule.

[0071] 2. Method

[0072] The field test was carried out at the ginseng planting base of Qinghe Forestry Bureau, Heilongjiang Provi...

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Abstract

Belonging to the field of agricultural microbiology, the invention in particular discloses three new bacillus strains, a bacillus composition and use thereof in control of panax ginseng and American ginseng black spot disease and botrytis cinerea. The bacillus combined inoculums provided by the invention can effectively control panax ginseng and American ginseng black spot disease and botrytis cinerea lesions, and has the characteristics of good field planting effect, long duration, lasting effect, and control effect superior to that of traditional chemical pesticides.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and in particular relates to a spore composition for preventing and treating black spot and gray mold of ginseng and American ginseng and its application. Background technique [0002] Ginseng is an important and precious Chinese medicinal material in my country. The wild resources are basically exhausted, and people basically rely on cultivation to meet people's needs. Ginseng has high requirements on soil, water, temperature, light, etc., and is extremely harsh on the environment. Therefore, ginseng cultivation technology is relatively high, and the production cost is also high. Even under suitable growth environment conditions, ginseng often suffers from diseases, resulting in large-scale yield reduction or even failure. If the soil, water, temperature, light and other conditions in the cultivation of ginseng cannot better meet the growth requirements of ginseng, the occurrence of di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01N63/02A01P3/00
CPCA01N63/00A01N63/10A01N2300/00
Inventor 马伟张腾霄孟祥才张连学许永华刘秀波徐姣
Owner HEILONGJIANG UNIV OF CHINESE MEDICINE
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