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Culture medium for simultaneously counting lactobacillus rhamnosus and lactobacillus acidophilus and application of culture medium

A technology of Lactobacillus rhamnosus and Lactobacillus acidophilus, which is applied in the direction of microorganism-based methods, measurement/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of high cost of experimental utensils, experimental reagents and human resources, Achieve the effect of improving work efficiency

Active Publication Date: 2017-12-29
完美(广东)日用品有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the defects that the counting method of lactic acid bacteria products in the prior art can only be counted for a single bacterial species, resulting in high costs of experimental vessels, experimental reagents and human resources, and then provide a mixed sample A method for counting Lactobacillus rhamnosus and Lactobacillus acidophilus at the same time, thereby reducing the cost of experimental utensils, experimental reagents and human resources, improving work efficiency and making it beneficial for enterprises to count lactic acid bacteria species

Method used

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  • Culture medium for simultaneously counting lactobacillus rhamnosus and lactobacillus acidophilus and application of culture medium
  • Culture medium for simultaneously counting lactobacillus rhamnosus and lactobacillus acidophilus and application of culture medium
  • Culture medium for simultaneously counting lactobacillus rhamnosus and lactobacillus acidophilus and application of culture medium

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Embodiment 1

[0032] The method for simultaneously counting Lactobacillus rhamnosus and Lactobacillus acidophilus in the mixed sample to be tested provided in this embodiment comprises the following steps,

[0033] Preparation of medium: Melt MRS agar and keep it warm in a water bath at 46°C, then add 1mL of clindamycin hydrochloride solution with a concentration of 0.005mg / mL to 100mL of MRS agar kept at 46°C to obtain a medium ;

[0034] (1) Take 8 mL of lincomycin hydrochloride modified MRS agar medium maintained at 46°C and pour it into a sterile plate and place it on the solidified base layer of the sandwich plate;

[0035] (2) The standard strains of Lactobacillus rhamnosus, Lactobacillus acidophilus, Bifidobacterium and Streptococcus thermophilus were respectively enriched for 48 hours, and 1 mL of each of the above four enrichment solutions were mixed as a mixed sample, and The mixed samples were serially diluted 10 times to 10 -6 Gradient concentration, and then take the above di...

Embodiment 2

[0040] The method for simultaneously counting Lactobacillus rhamnosus and Lactobacillus acidophilus in the mixed sample to be tested provided in this embodiment comprises the following steps,

[0041] Mixed bacterial powder sample A is uniformly mixed with freeze-dried bacterial powder of Lactobacillus rhamnosus, Lactobacillus acidophilus, Bifidobacterium and Streptococcus thermophilus;

[0042] Preparation of medium: Melt MRS agar and keep it warm in a water bath at 45°C, then add 1mL of clindamycin hydrochloride solution with a concentration of 0.01mg / mL to 100mL of MRS agar kept at 45°C to obtain a medium ;

[0043] (1) Take 10 mL of lincomycin hydrochloride modified MRS agar medium maintained at 45°C and pour it into a sterile plate and place it on the solidified base layer of the sandwich plate;

[0044] (2) The mixed sample A of Lactobacillus rhamnosus, Lactobacillus acidophilus, Bifidobacterium and Streptococcus thermophilus was serially diluted 10 times to 10 -6 Grad...

Embodiment 3

[0057] The method for simultaneously counting Lactobacillus rhamnosus and Lactobacillus acidophilus in the mixed sample to be tested provided in this embodiment comprises the following steps,

[0058] Mixed bacteria powder sample B is made by mixing the freeze-dried bacteria powder of Lactobacillus rhamnosus, Lactobacillus acidophilus, Bifidobacterium and Streptococcus thermophilus;

[0059] Preparation of culture medium: Melt MRS agar and incubate in a water bath at 55°C, then add 1.2mL of clindamycin hydrochloride solution with a concentration of 0.006mg / mL to 100mL of MRS agar kept at 55°C to obtain a culture medium base;

[0060] (1) Take 5 mL of lincomycin hydrochloride modified MRS agar medium maintained at 55° C. and pour it into a sterile plate and place it on the solidified base layer of the sandwich plate;

[0061] (2) The mixed sample B of Lactobacillus rhamnosus, Lactobacillus acidophilus, Bifidobacterium and Streptococcus thermophilus was serially diluted 10 time...

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Abstract

The invention provides a culture medium for simultaneously counting lactobacillus rhamnosus and lactobacillus acidophilus, and application of the culture medium. The culture medium is formed by improving MRS agar through lincomycin hydrochloride, and the culture medium formed by improving MRS agar through lincomycin hydrochloride is initiatively applied to simultaneous counting of lactobacillus rhamnosus and lactobacillus acidophilus. The invention further provides a method for simultaneously counting lactobacillus rhamnosus and lactobacillus acidophilus by utilizing the culture medium. The method comprises the following steps: firstly laying and solidifying the culture medium, so as to obtain a base layer, adding a to-be-tested mixed sample and the culture medium onto the base layer, and performing laying and solidifying, so as to obtain a middle layer; then adding the culture medium to the middle layer, and performing laying and solidifying, so as to obtain a surface layer, ensuring that the base layer, the middle layer and the surface layer form an interlayer flat plate, finally performing anaerobic cultivation on the interlayer flat plate, and counting lactobacillus rhamnosus and lactobacillus acidophilus separately. The method reduces the costs of experiment vessels, experiment reagents and human resources.

Description

technical field [0001] The invention belongs to the technical field of strain counting, and in particular relates to a culture medium for simultaneously counting Lactobacillus rhamnosus and Lactobacillus acidophilus and an application thereof. Background technique [0002] Lactic acid bacteria have the effects of improving gastric function, adjusting intestinal flora, inhibiting the growth of pathogenic bacteria, improving protein and vitamin metabolism, preventing constipation, relieving lactose intolerance, anti-tumor, strengthening the immune system and lowering cholesterol, so they are increasingly used Add to food and health products. Lactobacillus acidophilus, Bifidobacterium, Lactobacillus rhamnosus, and Streptococcus thermophilus all belong to lactic acid bacteria. In order to ensure that the above-mentioned lactic acid bacteria can play a normal physiological and health function in the human body, food organizations and health product organizations require that lact...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12Q1/06C12R1/23C12R1/225
CPCC12Q1/045C12Q1/06
Inventor 高业成欧阳道福郁晓艺李家炜杨紫红颜建章胡瑞连
Owner 完美(广东)日用品有限公司
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