Method for detecting quantity of bacterium lacticum in mixed probiotic products

A technology of lactobacilli and probiotics, applied in the field of health care products, can solve the problems of unspecified detection and counting methods of lactobacilli in mixed probiotics, negative values ​​and large deviations in results, and inability to perform accurate detection, etc., to achieve effective detection and Effects of controlling, removing interference, and filling gaps in detection

Pending Publication Date: 2020-08-25
BY HEALTH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This inspection method has the defects of detecting negative values ​​and large deviations in results, and cannot perform accurate detection
[0004] At present, the inspection method of probiotics is mainly in accordance with GB 4789.35-2016 N

Method used

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  • Method for detecting quantity of bacterium lacticum in mixed probiotic products

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Embodiment 1

[0073] Take 24 batches of mixed probiotic samples (containing Bifidobacteria and Lactobacillus), weigh 25g of mixed probiotic powder for each batch of samples, put them in a homogenization cup or a 500mL centrifuge tube + glass beads, add 225mL sterile normal saline accurately, and mix For 5 minutes, take 1mL sample homogeneous solution, add it to 9mL sterile normal saline, and make 10-fold incremental dilution, choose 2~3 continuous appropriate dilutions, and draw 1mL sample homogeneous solution into a sterile petri dish for each dilution. Make two plates for each dilution. After the dilution is transferred into the plate, pour about 15mL of the MRS agar medium cooled to 48°C into the plate, rotate the plate to mix evenly, and incubate aerobically at 36°C±1°C for 72h±2h. After counting the number of all colonies on the plate to obtain the lactobacillus, it should be completed within 15 minutes from sample dilution to plate pouring, and the test results are shown in Table 2.

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Abstract

The invention discloses a method for detecting quantity of bacterium lacticum in mixed probiotic products. The method comprises the steps of (1) weighing mixed probiotic samples, adding bacteria-freenormal saline, and performing homogenizing; (2) taking sample homogenizing liquid, adding bacteria-free normal saline, and performing sequential and progressive diluting to an appropriate dilution degree; and (3) absorbing formed sample homogenizing liquid having the appropriate dilution degree to a bacteria-free culture dish, pouring an MRS agar culture medium into the culture dish, performing uniform mixing, performing aerobic culture at 35-37 DEG C for 70-72h, and after culture, counting the quantity of all colonies on a flat plate so as to obtain the quantity of bacterium lacticum. According to the method disclosed by the invention, a manner of performing aerobic culture on bacterium lacticum, only bacterium lacticum grow on a counting culture medium, growth of bifidobacteria is restrained, and interference of bifidobacteria on the detection of the quantity of bacterium lacticum is removed, so that the quantity of bacterium lacticum in the mixed probiotic products can be accuratelydetected, effective detection and control of the quantity of viable bacteria in bacterium lacticum in mixed probiotics can be realized, and the method can fill the blank of the detection of the quantity of bacterium lacticum in mixed probiotics containing bifidobacteria and bacterium lacticum.

Description

technical field [0001] The invention belongs to the technical field of health products, and in particular relates to a method for detecting the number of lactobacillus in a mixed probiotic product. Background technique [0002] Probiotics have health effects such as regulating the balance of intestinal flora, inhibiting intestinal bacterial infection, preventing and treating tumors, lowering serum cholesterol levels, promoting digestion, and improving the body's immune function. They are widely used in the fields of fermented products and biological products. The number of probiotics is an important condition for a product to exert its probiotic effect, and the number of probiotics is often used as an evaluation index in product safety and functional tests. Therefore, the detection of probiotic count is very important for enterprise quality control and daily supervision. [0003] GB 4789.35-2010 National Food Safety Standard "Food Microbiological Examination of Lactic Acid ...

Claims

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Application Information

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IPC IPC(8): C12Q1/06C12R1/225
CPCC12Q1/06
Inventor 吴家碧李露蔡伟江
Owner BY HEALTH CO LTD
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