Optimized process method for improving stability of lactic acid bacteria cells

A process method and stability technology, applied in the field of lactic acid bacteria, can solve the problems of reduced number of viable bacteria and low stability, and achieve the effect of improving stability and extending shelf life

Active Publication Date: 2021-07-06
河北一然生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The present invention proposes an optimized process method for improving the stability of lactic acid bacteria cells, whi

Method used

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  • Optimized process method for improving stability of lactic acid bacteria cells
  • Optimized process method for improving stability of lactic acid bacteria cells
  • Optimized process method for improving stability of lactic acid bacteria cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] This example uses Lactobacillus plantarum Lp45.

[0038] test group:

[0039] S1. Spread the bacterial strain on the plate on the improved MRS agar solid medium, culture it upside down at 37°C for 48h, and screen for colonies with a smaller calcium-dissolving circle than 10% or less. The improved MRS agar solid medium includes the following groups Divide: peptone 8g, beef extract 8g, yeast extract 5g, diammonium hydrogen citrate 3g, glucose 15g, Tween 80 0.5ml, sodium acetate 4g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.6g, manganese sulfate 0.2g, agar 20g, 0.4g of L-cysteine ​​hydrochloride, 1L of water, the pH is controlled at 6.4 ± 0.1, 8g of calcium carbonate is also added to the improved MRS agar medium; the screening is repeated 20 times;

[0040] S2. Fermentation and cultivation after the tertiary seed cultivation of the bacterium obtained in S1, the tertiary cultivation is respectively:

[0041] (1) First-level seed culture: Pick a single colony ...

Embodiment 2

[0054] This example uses Lactobacillus acidophilus La28.

[0055] test group:

[0056] S1. Spread the bacterial strain on the plate on the improved MRS agar solid medium, culture it upside down at 37°C for 48h, and screen for colonies with a smaller calcium-dissolving circle than 10% or less. The improved MRS agar solid medium includes the following groups Divide: peptone 10g, beef extract 10g, yeast extract 4g, diammonium hydrogen citrate 2g, glucose 20g, Tween 80 1ml, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.5g, manganese sulfate 0.3g, agar 15g , 0.8g of L-cysteine ​​hydrochloride, 1L of water, the pH is controlled at 6.3 ± 0.1, 12g of calcium carbonate is also added to the improved MRS agar medium, and the screening is repeated 25 times;

[0057] S2. Fermentation and cultivation after the tertiary seed cultivation of the bacterium obtained in S1, the tertiary cultivation is respectively:

[0058] (1) First-level seed culture: pick a single...

Embodiment 3

[0071] This example uses Lactobacillus plantarum Lp45.

[0072] test group:

[0073] S1. Spread the bacterial strain on the plate on the improved MRS agar solid medium, culture it upside down at 37°C for 48h, and screen for colonies with a smaller calcium-dissolving circle than 10% or less. The improved MRS agar solid medium includes the following groups Divide: peptone 8g, beef extract 8g, yeast extract 5g, diammonium hydrogen citrate 3g, glucose 15g, Tween 80 0.5ml, sodium acetate 4g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.6g, manganese sulfate 0.2g, agar 20g, 0.4g of L-cysteine ​​hydrochloride, 1L of water, the pH is controlled at 6.4 ± 0.1, and 8g of calcium carbonate is also added in the improved MRS agar medium;

[0074] S2. Fermentation and cultivation after the tertiary seed cultivation of the bacterium obtained in S1, the tertiary cultivation is respectively:

[0075] (1) First-level seed culture: Pick a single colony from the solid plate medium into...

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Abstract

The invention relates to the technical field of lactic acid bacteria, and provides an optimized process method for improving the cell stability of lactic acid bacteria, which comprises the following steps: S1, culturing strains on an improved MRS agar culture medium solid plate, and screening thallus; S2, culturing the thallus seeds obtained in the step S1, then performing fermentation culture, and performing heat stress treatment on the fermentation liquid in the logarithmic later stage of fermentation; S3, centrifuging the fermentation liquid obtained in S2, and collecting bacterial sludge; S4, uniformly mixing the bacterial sludge obtained in S3 with the fermentation supernatant to obtain an emulsion; S5, freeze-drying the emulsion obtained in S4, and collecting bacterial powder; S6, repeating the operations of S1 to S5 on the bacterial powder obtained in S5 for 20-25 times to obtain stable bacterial powder; and S7, continuously culturing, centrifuging, emulsifying and freeze-drying the stable bacterial powder obtained in S6 to obtain final bacterial powder. According to the technical scheme, the problems that in the prior art, the viable count of lactic acid bacteria in the storage process is reduced, and the stability is not high are solved.

Description

technical field [0001] The invention relates to the technical field of lactic acid bacteria, in particular to an optimized process method for improving the stability of lactic acid bacteria cells. Background technique [0002] Probiotics are live microorganisms that, when ingested in sufficient quantities by humans or animals, have beneficial effects on health. In recent years, with people's understanding of new probiotics, the global demand for functional foods containing probiotics has increased significantly. However, probiotics are very fragile and are easily affected by the storage environment and lose their activity. How to keep the number of live bacteria of probiotic products unchanged during storage, give full play to their efficacy, expand their application range and improve their stability needs to be solved urgently. Contents of the invention [0003] The invention proposes an optimized process method for improving the stability of lactic acid bacteria cells, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04C12R1/01C12R1/225C12R1/46
CPCC12N1/20C12N1/04C12N1/005
Inventor 郭红敏杨玲赵林森申朋贾洪利仵红岩任磊霍世和马新颖孙策
Owner 河北一然生物科技股份有限公司
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